1921
Volume 86, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

North African gundis () were trapped in the () focus of cutaneous leishmaniasis, situated in southeast Tunisia and evaluated for infection by real-time kinetoplast DNA polymerase chain reaction (PCR). Species identification was performed by internal transcribed spacer one (ITS1)-PCR-restriction fragment length polymorphism (RFLP) and high-resolution melting (HRM) analysis of the 7SL RNA gene. Real-time PCR on blood was positive in 6 of 13 (46.2%) tested gundis. was identified in five infected gundis and in one specimen. Alignments of the ITS-1 DNA sequences and 7S-HRM curves analysis indicated that similar genotypes were present in humans, a sandfly, and gundis from the same region suggesting a potential role of this rodent as reservoir host of in southeast Tunisia.

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2012-06-01
2017-09-24
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  • Received : 10 Sep 2011
  • Accepted : 04 Jan 2012

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