Volume 87, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Small mammals from the Democratic Republic (DR) of the Congo and Tanzania were tested to determine the prevalence and genetic diversity of species. The presence of DNA was assessed in spleen samples of the animals by B- and Apolymerase chain reactions (PCRs). By BPCR, was detected in 8 of 59 animals of DR Congo and in 16 of 39 Tanzanian animals. By APCR, was detected in 5 and 15 animals of DR Congo and Tanzania, respectively. The gene sequences from were closely related to . The genotypes from spp. and from were close to . Five genogroups were not genetically related to any known species. These results suggest the need to conduct further studies to establish the zoonotic risks linked with those species and, in particular, to verify whether these agents might be responsible for human cases of febrile illness of unknown etiology in Africa.


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  1. Breitschwerdt EB, Kordick DL, , 2000. Bartonella infection in animals: carriership, reservoir potential, pathogenicity, and zoonotic potential for human infection. Clin Microbiol Rev 13: 428438.[Crossref] [Google Scholar]
  2. Chomel BB, Boulouis HJ, Maruyama S, Breitschwerdt EB, , 2006. Bartonella spp. in pets and effect on human health. Emerg Infect Dis 12: 389394.[Crossref] [Google Scholar]
  3. Mogollon-Pasapera E, Otvos L, Jr Giordano A, Cassone M, , 2009. Bartonella: emerging pathogen or emerging awareness? Int J Infect Dis 13: 38.[Crossref] [Google Scholar]
  4. Koehler JE, , 1996. Bartonella infections. Adv Pediatr Infect Dis 11: 127. [Google Scholar]
  5. Kosoy MY, Regnery RL, Tzianabos T, Marston EL, Jones DC, Green D, Maupin GO, Olson JG, Childs JE, , 1997. Distribution, diversity, and host specificity of Bartonella in rodents from the southeastern United States. Am J Trop Med Hyg 57: 578588. [Google Scholar]
  6. Heller R, Riegel P, Hansmann Y, Delacour G, Bermond D, Dehio C, Lamarque F, Monteil H, Chomel B, Piemont Y, , 1998. Bartonella tribocorum sp. nov., a new Bartonella species isolated from the blood of wild rats. Int J Syst Bacteriol 48: 13331339.[Crossref] [Google Scholar]
  7. Hofmeister EK, Kolbert CP, Abdulkarim AS, Magera JM, Hopkins MK, Uhl JR, Ambyaye A, Telford SR, 3rd Cockerill FR, 3rd Persing DH, , 1998. Cosegregation of a novel Bartonella species with Borrelia burgdorferi and Babesia microti in Peromyscus leucopus . J Infect Dis 177: 409416.[Crossref] [Google Scholar]
  8. Birtles RJ, Hazel SM, Bennett M, Bown K, Raoult D, Begon M, , 2001. Longitudinal monitoring of the dynamics of infections due to Bartonella species in UK woodland rodents. Epidemiol Infect 126: 323329.[Crossref] [Google Scholar]
  9. Holmberg M, Mills JN, McGill S, Benjamin G, Ellis BA, , 2003. Bartonella infection in sylvatic small mammals of central Sweden. Epidemiol Infect 130: 149157.[Crossref] [Google Scholar]
  10. Tea A, Alexiou-Daniel S, Papoutsi A, Papa A, Antoniadis A, , 2004. Bartonella species isolated from rodents, Greece. Emerg Infect Dis 10: 963964.[Crossref] [Google Scholar]
  11. Gundi VA, Davoust B, Khamis A, Boni M, Raoult D, La Scola B, , 2004. Isolation of Bartonella rattimassiliensis sp. nov. and Bartonella phoceensis sp. nov. from European Rattus norvegicus . J Clin Microbiol 42: 38163818.[Crossref] [Google Scholar]
  12. Jardine C, Appleyard G, Kosoy MY, McColl D, Chirino-Trejo M, Wobeser G, Leighton FA, , 2005. Rodent-associated Bartonella in Saskatchewan, Canada. Vector Borne Zoonotic Dis 5: 402409.[Crossref] [Google Scholar]
  13. Knap N, Duh D, Birtles R, Trilar T, Petrovec M, Avsic-Zupanc T, , 2007. Molecular detection of Bartonella species infecting rodents in Slovenia. FEMS Immunol Med Microbiol 50: 4550.[Crossref] [Google Scholar]
  14. Pretorius AM, Beati L, Birtles RJ, , 2004. Diversity of bartonellae associated with small mammals inhabiting Free State province, South Africa. Int J Syst Evol Microbiol 54: 19591967.[Crossref] [Google Scholar]
  15. Gundi VA, Taylor C, Raoult D, La Scola B, , 2009. Bartonella rattaustraliani sp. nov., Bartonella queenslandensis sp. nov. and Bartonella coopersplainsensis sp. nov., identified in Australian rats. Int J Syst Evol Microbiol 59: 29562961.[Crossref] [Google Scholar]
  16. Gundi VA, Bourry O, Davous B, Raoult D, La Scola B, , 2004. Bartonella clarridgeiae and B. henselae in dogs, Gabon. Emerg Infect Dis 10: 22612262. [Google Scholar]
  17. Heller R, Kubina M, Mariet P, Riegel P, Delacour G, Dehio C, Lamarque F, Kasten R, Boulouis HJ, Monteil H, Chomel B, Piemont Y, , 1999. Bartonella alsatica sp. nov., a new Bartonella species isolated from the blood of wild rabbits. Int J Syst Bacteriol 49: 283288.[Crossref] [Google Scholar]
  18. Kordick DL, Swaminathan B, Greene CE, Wilson KH, Whitney AM, O'Connor S, Hollis DG, Matar GM, Steigerwalt AG, Malcolm GB, Hayes PS, Hadfield TL, Breitschwerdt EB, Brenner DJ, , 1996. Bartonella vinsonii subsp. berkhoffii subsp. nov., isolated from dogs; Bartonella vinsonii subsp. vinsonii; and emended description of Bartonella vinsonii . Int J Syst Bacteriol 46: 704709.[Crossref] [Google Scholar]
  19. Chang CC, Chomel BB, Kasten RW, Heller RM, Ueno H, Yamamoto K, Bleich VC, Pierce BM, Gonzales BJ, Swift PK, Boyce WM, Jang SS, Boulouis HJ, Piemont Y, Rossolini GM, Riccio ML, Cornaglia G, Pagani L, Lagatolla C, Selan L, Fontana R, , 2000. Bartonella spp. isolated from wild and domestic ruminants in North America. Emerg Infect Dis 6: 306311.[Crossref] [Google Scholar]
  20. Castle KT, Kosoy M, Lerdthusnee K, Phelan L, Bai Y, Gage KL, Leepitakrat W, Monkanna T, Khlaimanee N, Chandranoi K, Jones JW, Coleman RE, , 2004. Prevalence and diversity of Bartonella in rodents of northern Thailand: a comparison with Bartonella in rodents from southern China. Am J Trop Med Hyg 70: 429433. [Google Scholar]
  21. Ellis BA, Regnery RL, Beati L, Bacellar F, Rood M, Glass GG, Marston E, Ksiazek TG, Jones D, Childs JE, , 1999. Rats of the genus Rattus are reservoir hosts for pathogenic Bartonella species: an Old World origin for a New World disease? J Infect Dis 180: 220224.[Crossref] [Google Scholar]
  22. Ying B, Kosoy MY, Maupin GO, Tsuchiya KR, Gage KL, , 2002. Genetic and ecologic characteristics of Bartonella communities in rodents in southern China. Am J Trop Med Hyg 66: 622627. [Google Scholar]
  23. Fournier PE, Robson J, Zeaiter Z, McDougall R, Byrne S, Raoult D, , 2002. Improved culture from lymph nodes of patients with cat scratch disease and genotypic characterization of Bartonella henselae isolates in Australia. J Clin Microbiol 40: 36203624.[Crossref] [Google Scholar]
  24. Engbaek K, Lawson PA, , 2004. Identification of Bartonella species in rodents, shrews and cats in Denmark: detection of two B. henselae variants, one in cats and the other in the long-tailed field mouse. APMIS 112: 336341.[Crossref] [Google Scholar]
  25. De Sousa R, Edouard-Fournier P, Santos-Silva M, Amaro F, Bacellar F, Raoult D, , 2006. Molecular detection of Rickettsia felis, Rickettsia typhi and two genotypes closely related to Bartonella elizabethae . Am J Trop Med Hyg 75: 727731. [Google Scholar]
  26. Marie JL, Fournier PE, Rolain JM, Briolant S, Davoust B, Raoult D, , 2006. Molecular detection of Bartonella quintana, B. Elizabethae, B. koehlerae, B. doshiae, B. taylorii, and Rickettsia felis in rodent fleas collected in Kabul, Afghanistan. Am J Trop Med Hyg 74: 436439. [Google Scholar]
  27. Gundi VA, Kosoy MY, Myint KS, Shrestha SK, Shrestha MP, Pavlin JA, Gibbons RV, , 2010. Prevalence and genetic diversity of Bartonella species detected in different tissues of small mammals in Nepal. Appl Environ Microbiol 76: 82478254.[Crossref] [Google Scholar]
  28. Sackal C, Laudisoit A, Kosoy M, Massung R, Eremeeva ME, Karpathy SE, Van Wyk K, Gabitzsch E, Zeidner NS, , 2008. Bartonella spp. and Rickettsia felis in fleas, Democratic Republic of Congo. Emerg Infect Dis 14: 19721974.[Crossref] [Google Scholar]
  29. Renesto P, Gouvernet J, Drancourt M, Roux V, Raoult D, , 2001. Use of rpoB gene analysis for detection and identification of Bartonella species. J Clin Microbiol 39: 430437.[Crossref] [Google Scholar]
  30. Birtles RJ, Raoult D, , 1996. Comparison of partial citrate synthase gene (gltA) sequences for phylogenetic analysis of Bartonella species. Int J Syst Bacteriol 46: 891897.[Crossref] [Google Scholar]
  31. Norman AF, Regnery R, Jameson P, Greene C, Krause DC, , 1995. Differentiation of Bartonella-like isolates at the species level by PCR-restriction fragment length polymorphism in the citrate synthase gene. J Clin Microbiol 33: 17971803. [Google Scholar]
  32. Tamura K, Dudley J, Nei M, Kumar S, , 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: 15961599.[Crossref] [Google Scholar]
  33. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG, , 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 48764882.[Crossref] [Google Scholar]
  34. Saitou N, Nei M, , 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406425. [Google Scholar]
  35. Kimura M, , 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111120.[Crossref] [Google Scholar]
  36. Felsenstein J, , 1992. Estimating effective population size from samples of sequences: a bootstrap Monte Carlo integration method. Genet Res 60: 209220.[Crossref] [Google Scholar]
  37. La Scola B, Zeaiter Z, Khamis A, Raoult D, , 2003. Gene-sequence-based criteria for species definition in bacteriology: the Bartonella paradigm. Trends Microbiol 11: 318321.[Crossref] [Google Scholar]
  38. Inoue K, Maruyama S, Kabeya H, Yamada N, Ohashi N, Sato Y, Yukawa M, Masuzawa T, Kawamori F, Kadosaka T, Takada N, Fujita H, Kawabata H, , 2008. Prevalence and genetic diversity of Bartonella species isolated from wild rodents in Japan. Appl Environ Microbiol 74: 50865092.[Crossref] [Google Scholar]
  39. Bai Y, Montgomery SP, Sheff KW, Chowdhury MA, Breiman RF, Kabeya H, Kosoy MY, , 2007. Bartonella strains in small mammals from Dhaka, Bangladesh, related to Bartonella in America and Europe. Am J Trop Med Hyg 77: 567570. [Google Scholar]
  40. Marquez FJ, Rodriguez-Liebana JJ, Pachon-Ibanez ME, Docobo-Perez F, Hidalgo-Fontiveros A, Bernabeu-Wittel M, Muniain MA, Pachon J, , 2008. Molecular screening of Bartonella species in rodents from south western Spain. Vector Borne Zoonotic Dis 8: 695700.[Crossref] [Google Scholar]
  41. Welc-Faleciak R, Paziewska A, Bajer A, Behnke JM, Sinski E, , 2008. Bartonella spp. infection in rodents from different habitats in the Mazury Lake District, Northeast Poland. Vector Borne Zoonotic Dis 8: 467474.[Crossref] [Google Scholar]

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  • Received : 31 Aug 2011
  • Accepted : 04 Jan 2012
  • Published online : 01 Aug 2012

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