Volume 87, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We investigated the occurrence of in opossums at a wild animal rehabilitation center in the city of Campo Grande, Brazil. A total of 54 opossums were tested for infection in peripheral blood and bone marrow samples. The samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction–restriction fragment length polymorphism. DNA was detected by polymerase chain reaction–restriction fragment length polymorphism in 11 (20.37%) animals. A total of 81.81% of positive opossums were captured in areas of known visceral leishmaniasis transmission. These results suggest a role for in the urban transmission of visceral leishmaniasis.


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  • Received : 17 Aug 2011
  • Accepted : 25 Mar 2012

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