1921
Volume 86, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Two hundred and three isolates from Jazan area, southwest Saudi Arabia, were typed for , , , and mutations associated with resistance to chloroquine, mefloquine, halofantrine, artemisinin, sulfadoxine-pyrimethamine, and the neutral polymorphic gene . A large proportion (33%) of isolates harbored double mutant genotype (51,59C,108). However, only one isolate contained mutation -437. For , almost all examined isolates (163; 99%) harbored the mutant genotype (72C,73V,74I,75E,76), whereas only 49 (31%) contained the mutant genotype (86,184,1034S,1042N), 109 (66%) harbored the single mutant genotype (86N,184,1034S,1042N), and no mutations were seen in codons 1034, 1042, and 1246. Nonetheless, three new single-nucleotide polymorphisms were detected at codons 182, 192, and 102. No differences were seen in distribution of drug resistance genes among Saudis and expatriates. There was a limited multiplicity (5%), mean number of clones (1.05), and two dominant multilocus genotypes among infected individuals in Jazan. A pattern consistent with limited cross-mating and recombination among local parasite was apparent.

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  • Received : 11 Aug 2011
  • Accepted : 21 Oct 2011

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