Volume 86, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



sibling species ( s.s., , and species complex [eight cryptic species]) are principal vectors of malaria and filariasis in the Southwest Pacific. Given significant effort to reduce malaria and filariasis transmission through insecticide-treated net distribution in the region, effective strategies to monitor evolution of insecticide resistance among . sibling species is essential. Mutations in the voltage-gated sodium channel () gene have been associated with knock-down resistance () to pyrethroids and DDT in malarious regions. By examining sequence polymorphism we developed a multiplex assay to differentiate wild-type versus alleles and query intron-based polymorphisms that enable simultaneous species identification. A survey including mosquitoes from seven Papua New Guinea Provinces detected no alleles in any species. Absence of sequence introgression between species and evidence of geographic separation within species suggests that must be monitored in each . species independently.


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  • Received : 03 Aug 2011
  • Accepted : 24 Sep 2011
  • Published online : 01 Jan 2012

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