1921
Volume 86, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The malaria vaccine RTS,S/AS01, based on immunogenic regions of the circumsporozoite protein (CSP), has partial efficacy against clinical malaria in African children. Understanding how sequence diversity in CSP T- and B-cell epitopes relates to naturally acquired and vaccine-induced immunity may be useful in efforts to improve the efficacy of CSP-based vaccines. However, limitations in sequencing technology have precluded thorough evaluation of diversity in the immunogenic regions of this protein. In this study, 454, a next generation sequencing technology, was evaluated as a method for assessing diversity in these regions. Portions of the circumsporozoite gene () were sequenced both by 454 and Sanger sequencing from samples collected in a study in Bandiagara, Mali. 454 detected more single nucleotide polymorphisms and haplotypes in the T-cell epitopes than Sanger sequencing, and it was better able to resolve genetic diversity in samples with multiple infections; however, it failed to generate sequence for the B-cell epitopes.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2012.11-0478
2012-05-01
2017-11-19
Loading full text...

Full text loading...

/deliver/fulltext/14761645/86/5/775.html?itemId=/content/journals/10.4269/ajtmh.2012.11-0478&mimeType=html&fmt=ahah

References

  1. Takala SL, Plowe CV, , 2009. Genetic diversity and malaria vaccine design, testing and efficacy: preventing and overcoming ‘vaccine resistant malaria’. Parasite Immunol 31: 560573.[Crossref]
  2. Escalante AA, Grebert HM, Isea R, Goldman IF, Basco L, Magris M, Biswas S, Kariuki S, Lal AA, , 2002. A study of genetic diversity in the gene encoding the circumsporozoite protein (CSP) of Plasmodium falciparum from different transmission areas–XVI. Asembo Bay Cohort Project. Mol Biochem Parasitol 125: 8390.[Crossref]
  3. Egan JE, Hoffman SL, Haynes JD, Sadoff JC, Schneider I, Grau GE, Hollingdale MR, Ballou WR, Gordon DM, , 1993. Humoral immune responses in volunteers immunized with irradiated Plasmodium falciparum sporozoites. Am J Trop Med Hyg 49: 166173.
  4. Nardin EH, Nussenzweig RS, McGregor IA, Bryan JH, , 1979. Antibodies to sporozoites: their frequent occurrence in individuals living in an area of hyperendemic malaria. Science 206: 597599.[Crossref]
  5. de Groot AS, Johnson AH, Maloy WL, Quakyi IA, Riley EM, Menon A, Banks SM, Berzofsky JA, Good MF, , 1989. Human T cell recognition of polymorphic epitopes from malaria circumsporozoite protein. J Immunol 142: 40004005.
  6. Chenet SM, Branch OH, Escalante AA, Lucas CM, Bacon DJ, , 2008. Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the amazon basin of peru. Malar J 7: 93.[Crossref]
  7. Weedall GD, Preston BM, Thomas AW, Sutherland CJ, Conway DJ, , 2007. Differential evidence of natural selection on two leading sporozoite stage malaria vaccine candidate antigens. Int J Parasitol 37: 7785.[Crossref]
  8. Jalloh A, Jalloh M, Matsuoka H, , 2009. T-cell epitope polymorphisms of the Plasmodium falciparum circumsporozoite protein among field isolates from sierra leone: age-dependent haplotype distribution? Malar J 8: 120.[Crossref]
  9. Bojang KA, Milligan PJ, Pinder M, Vigneron L, Alloueche A, Kester KE, Ballou WR, Conway DJ, Reece WH, Gothard P, Yamuah L, Delchambre M, Voss G, Greenwood BM, Hill A, McAdam KP, Tornieporth N, Cohen JD, Doherty T, RTS, S Malaria Vaccine Trial Team, 2001. Efficacy of RTS,S/AS02 malaria vaccine against Plasmodium falciparum infection in semi-immune adult men in the Gambia: a randomised trial. Lancet 358: 19271934.[Crossref]
  10. Alonso PL, Sacarlal J, Aponte JJ, Leach A, Macete E, Milman J, Mandomando I, Spiessens B, Guinovart C, Espasa M, Bassat Q, Aide P, Ofori-Anyinam O, Navia MM, Corachan S, Ceuppens M, Dubois MC, Demoitié MA, Dubovsky F, Menéndez C, Tornieporth N, Ballou WR, Thompson R, Cohen J, , 2004. Efficacy of the RTS,S/AS02A vaccine against Plasmodium falciparum infection and disease in young african children: randomised controlled trial. Lancet 364: 14111420.[Crossref]
  11. Aponte JJ, Aide P, Renom M, Mandomando I, Bassat Q, Sacarlal J, Manaca MN, Lafuente S, Barbosa A, Leach A, Lievens M, Vekemans J, Sigauque B, Dubois MC, Demoitié MA, Sillman M, Savarese B, McNeil JG, Macete E, Ballou WR, Cohen J, Alonso PL, , 2007. Safety of the RTS,S/AS02D candidate malaria vaccine in infants living in a highly endemic area of mozambique: a double blind randomised controlled phase I/IIb trial. Lancet 370: 15431551.[Crossref]
  12. Bejon P, Lusingu J, Olotu A, Leach A, Lievens M, Vekemans J, Mshamu S, Lang T, Gould J, Dubois MC, Demoitié MA, Stallaert JF, Vansadia P, Carter T, Njuguna P, Awuondo KO, Malabeja A, Abdul O, Gesase S, Mturi N, Drakeley CJ, Savarese B, Villafana T, Ballou WR, Cohen J, Riley EM, Lemnge MM, Marsh K, von Seidlein L, , 2008. Efficacy of RTS,S/AS01E vaccine against malaria in children 5 to 17 months of age. N Engl J Med 359: 25212532.[Crossref]
  13. Agnandji ST, Lell B, Soulanoudjingar SS, Fernandes JF, Abossolo BP, Conzelmann C, Methogo BG, Doucka Y, Flamen A, Mordmüller B, Issifou S, Kremsner PG, Sacarlal J, Aide P, Lanaspa M, Aponte JJ, Nhamuave A, Quelhas D, Bassat Q, Mandjate S, Macete E, Alonso P, Abdulla S, Salim N, Juma O, Shomari M, Shubis K, Machera F, Hamad AS, Minja R, Mtoro A, Sykes A, Ahmed S, Urassa AM, Ali AM, Mwangoka G, Tanner M, Tinto H, D'Alessandro U, Sorgho H, Valea I, Tahita MC, Kaboré W, Ouédraogo S, Sandrine Y, Guiguemdé RT, Ouédraogo JB, Hamel MJ, Kariuki S, Odero C, Oneko M, Otieno K, Awino N, Omoto J, Williamson J, Muturi-Kioi V, Laserson KF, Slutsker L, Otieno W, Otieno L, Nekoye O, Gondi S, Otieno A, Ogutu B, Wasuna R, Owira V, Jones D, Onyango AA, Njuguna P, Chilengi R, Akoo P, Kerubo C, Gitaka J, Maingi C, Lang T, Olotu A, Tsofa B, Bejon P, Peshu N, Marsh K, Owusu-Agyei S, Asante KP, Osei-Kwakye K, Boahen O, Ayamba S, Kayan K, Owusu-Ofori R, Dosoo D, Asante I, Adjei G, Adjei G, Chandramohan D, Greenwood B, Lusingu J, Gesase S, Malabeja A, Abdul O, Kilavo H, Mahende C, Liheluka E, Lemnge M, Theander T, Drakeley C, Ansong D, Agbenyega T, Adjei S, Boateng HO, Rettig T, Bawa J, Sylverken J, Sambian D, Agyekum A, Owusu L, Martinson F, Hoffman I, Mvalo T, Kamthunzi P, Nkomo R, Msika A, Jumbe A, Chome N, Nyakuipa D, Chintedza J, Ballou WR, Bruls M, Cohen J, Guerra Y, Jongert E, Lapierre D, Leach A, Lievens M, Ofori-Anyinam O, Vekemans J, Carter T, Leboulleux D, Loucq C, Radford A, Savarese B, Schellenberg D, Sillman M, Vansadia P, RTS,S Clinical Trials Partnership, 2011. First results of phase 3 trial of RTS,S/AS01 malaria vaccine in African children. N Engl J Med 365: 18631875.[Crossref]
  14. Alloueche A, Milligan P, Conway DJ, Pinder M, Bojang K, Doherty T, Tornieporth N, Cohen J, Greenwood BM, , 2003. Protective efficacy of the RTS,S/AS02 Plasmodium falciparum malaria vaccine is not strain specific. Am J Trop Med Hyg 68: 97101.
  15. Carr IM, Robinson JI, Dimitriou R, Markham AF, Morgan AW, Bonthron DT, , 2009. Inferring relative proportions of DNA variants from sequencing electropherograms. Bioinformatics 25: 32443250.[Crossref]
  16. Coulibaly D, Diallo DA, Thera MA, Dicko A, Guindo AB, Koné AK, Cissoko Y, Coulibaly S, Djimdé A, Lyke K, Doumbo OK, Plowe CV, , 2002. Impact of preseason treatment on incidence of falciparum malaria and parasite density at a site for testing malaria vaccines in Bandiagara, Mali. Am J Trop Med Hyg 67: 604610.
  17. Williams R, Peisajovich SG, Miller OJ, Magdassi S, Tawfik DS, Griffiths AD, , 2006. Amplification of complex gene libraries by emulsion PCR. Nat Methods 3: 545550.[Crossref]
  18. Hunt P, Fawcett R, Carter R, Walliker D, , 2005. Estimating SNP proportions in populations of malaria parasites by sequencing: validation and applications. Mol Biochem Parasitol 143: 173182.[Crossref]
  19. Ochola LI, Tetteh KK, Stewart LB, Riitho V, Marsh K, Conway DJ, , 2010. Allele frequency-based and polymorphism-versus-divergence indices of balancing selection in a new filtered set of polymorphic genes in Plasmodium falciparum . Mol Biol Evol 27: 23442351.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2012.11-0478
Loading
/content/journals/10.4269/ajtmh.2012.11-0478
Loading

Data & Media loading...

  • Received : 23 Jul 2011
  • Accepted : 18 Jan 2012

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error