Volume 85, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Determining mosquito age is important to evaluate vector control programs because the ability to transmit diseases is age dependent. Current age-grading techniques require dissection or RNA extraction. Near infrared spectroscopy has been used to rapidly and nondestructively determine the age of fresh mosquitoes and specimens stored in RNA, but other preservation techniques have not been examined. Thus, in this study, we investigate whether age can be predicted from insects preserved by various common methods. Results from this study show that age can be predicted from mosquitoes preserved with desiccants, ethanol, Carnoy, RNA, or refrigeration with confidence intervals < 1.4 days. The best results were generally obtained from mosquitoes stored using desiccants, RNA, or refrigeration.


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  • Received : 07 Jul 2011
  • Accepted : 18 Aug 2011
  • Published online : 01 Dec 2011

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