Volume 85, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We molecularly characterized samples with , , and soil-transmitted helminths from a facility-based surveillance system for diarrhea in Santa Rosa, Guatemala. The DNA sequence analysis determined the presence of assemblages A ( = 7) and B ( = 12) and, ( = 2) and ( = 2), suggestive of different transmission cycles. All 41 samples with soil-transmitted helminths did not have the β-tubulin mutation described for benzimidazole resistance, suggesting potential usefulness in mass drug administration campaigns.


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  1. Jensen LA, Marlin JW, Dyck DD, Laubach HE, , 2009. Prevalence of multi-gastrointestinal infections with helminth, protozoan and Campylobacter spp. in Guatemalan children. J Infect Dev Ctries. 3: 229234.
  2. Cook DM, Swanson RC, Eggett DL, Booth GM, , 2009. A retrospective analysis of prevalence of gastrointestinal parasites among school children in the Palajunoj Valley of Guatemala. J Health Popul Nutr 27: 3140.[Crossref]
  3. Xiao L, Fayer R, , 2008. Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission. Int J Parasitol 38: 12391255.[Crossref]
  4. Xiao L, Fayer R, Ryan U, Upton SJ, , 2004. Cryptosporidium taxonomy: recent advances and implications for public health. Clin Microbiol Rev 17: 7297.[Crossref]
  5. Smith HV, Caccio SM, Tait A, McLauchlin J, Thompson RC, , 2006. Tools for investigating the environmental transmission of Cryptosporidium and Giardia infections in humans. Trends Parasitol 22: 160167.[Crossref]
  6. Feng Y, Xiao L, , 2011. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clin Microbiol Rev 24: 110140.[Crossref]
  7. Xiao L, , 2010. Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol 124: 8089.[Crossref]
  8. Hotez PJ, , 2009. Mass drug administration and integrated control for the world's high-prevalence neglected tropical diseases. Clin Pharmacol Ther 85: 659664.[Crossref]
  9. Bethony J, Brooker S, Albonico M, Geiger SM, Loukas A, Diemert D, Hotez PJ, , 2006. Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. Lancet 367: 15211532.[Crossref]
  10. Geerts S, Gryseels B, , 2001. Anthelmintic resistance in human helminths: a review. Trop Med Int Health 6: 915921.[Crossref]
  11. Albonico M, Wright V, Bickle Q, , 2004. Molecular analysis of the beta-tubulin gene of human hookworms as a basis for possible benzimidazole resistance on Pemba Island. Mol Biochem Parasitol 134: 281284.[Crossref]
  12. Kwa MS, Veenstra JG, Roos MH, , 1994. Benzimidazole resistance in Haemonchus contortus is correlated with a conserved mutation at amino acid 200 in beta-tubulin isotype 1. Mol Biochem Parasitol 63: 299303.[Crossref]
  13. Sulaiman IM, Fayer R, Bern C, Gilman RH, Trout JM, Schantz PM, Das P, Lal AA, Xiao L, , 2003. Triosephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis . Emerg Infect Dis 9: 14441452.[Crossref]
  14. Alves M, Xiao L, Sulaiman I, Lal AA, Matos O, Antunes F, , 2003. Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal. J Clin Microbiol 41: 27442747.[Crossref]
  15. Xiao L, Bern C, Limor J, Sulaiman I, Roberts J, Checkley W, Cabrera L, Gilman RH, Lal AA, , 2001. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J Infect Dis 183: 492497.[Crossref]
  16. Diawara A, Drake LJ, Suswillo RR, Kihara J, Bundy DA, Scott ME, Halpenny C, Stothard JR, Prichard RK, , 2009. Assays to detect beta-tubulin codon 200 polymorphism in Trichuris trichiura and Ascaris lumbricoides . PLoS Negl Trop Dis 3: e397.[Crossref]
  17. Lebbad M, Ankarklev J, Tellez A, Leiva B, Andersson JO, Svard S, , 2008. Dominance of Giardia assemblage B in Leon, Nicaragua. Acta Trop 106: 4453.[Crossref]
  18. Pelayo L, Nunez FA, Rojas L, Furuseth Hansen E, Gjerde B, Wilke H, Mulder B, Robertson L, , 2008. Giardia infections in Cuban children: the genotypes circulating in a rural population. Ann Trop Med Parasitol 102: 585595.[Crossref]
  19. Caccio SM, Ryan U, , 2008. Molecular epidemiology of giardiasis. Mol Biochem Parasitol 160: 7580.[Crossref]
  20. Peng MM, Matos O, Gatei W, Das P, Stantic-Pavlinic M, Bern C, Sulaiman IM, Glaberman S, Lal AA, Xiao L, , 2001. A comparison of Cryptosporidium subgenotypes from several geographic regions. J Eukaryot Microbiol (Suppl): 28S31S.[Crossref]
  21. Cama VA, Bern C, Roberts J, Cabrera L, Sterling CR, Ortega Y, Gilman RH, Xiao L, , 2008. Cryptosporidium species and subtypes and clinical manifestations in children, Peru. Emerg Infect Dis 14: 15671574.[Crossref]
  22. Bennett AB, Anderson TJ, Barker GC, Michael E, Bundy DA, , 2002. Sequence variation in the Trichuris trichiura beta-tubulin locus: implications for the development of benzimidazole resistance. Int J Parasitol 32: 15191528.[Crossref]
  23. Schwenkenbecher JM, Albonico M, Bickle Q, Kaplan RM, , 2007. Characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time PCR. Mol Biochem Parasitol 156: 167174.[Crossref]

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  • Received : 07 Jul 2011
  • Accepted : 12 Sep 2011

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