Volume 85, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The geographical distribution of genetic variation in samples ( = 386) from nine districts across Sri Lanka is described using three markers; the -3α (-3α) gene, and the two microsatellites m1501 and m3502. At -3α, 11 alleles were found with an expected heterozygosity (H) of 0.81, whereas at m1501 and m3502, 24 alleles (H = 0.85) and 8 alleles (H = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H = 0.95). Calculating the pairwise fixation index ( ) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of populations in Sri Lanka.


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  1. Mendis K, Sina BJ, Marchesini P, Carter R, , 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64: 97106. [Google Scholar]
  2. Mueller I, Galinski MR, Baird JK, Carlton JM, Kochar DK, Alonso PL, Del Portillo HA, , 2009. Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite. Lancet Infect Dis 9: 555566.[Crossref] [Google Scholar]
  3. Carlton JM, Adams JH, Silva JC, Bidwell SL, Lorenzi H, Caler E, Crabtree J, Angiuoli SV, Merino EF, Amedeo P, Cheng Q, Coulson RM, Crabb BS, del Portillo HA, Essien K, Feldblyum TV, Fernandez-Becerra C, Gilson PR, Gueye AH, Guo X, Kang'a S, Kooij TW, Korsinczky M, Meyer EV, Nene V, Paulsen I, White O, Ralph SA, Ren Q, Sargeant TJ, Salzberg SL, Stoeckert CJ, Sullivan SA, Yamamoto MM, Hoffman SL, Wortman JR, Gardner MJ, Galinski MR, Barnwell JW, Fraser-Liggett CM, , 2008. Comparative genomics of the neglected human malaria parasite Plasmodium vivax . Nature 455: 757763.[Crossref] [Google Scholar]
  4. Aresha M, Sanath M, Deepika F, Renu W, Anura B, Chanditha H, Wimaladharma A, Rajitha W, , 2008. Genotyping of Plasmodium vivax infections in Sri Lanka using Pvmsp-3alpha and Pvcs genes as markers: a preliminary report. Trop Biomed 25: 100106. [Google Scholar]
  5. World Health Organization, 2010. World Malaria Report 2010. Geneva: WHO. [Google Scholar]
  6. Dowdle WR, , 1998. The principles of disease elimination and eradication. Bull World Health Organ 76 (Suppl 2): 2225. [Google Scholar]
  7. Rajakaruna RS, Alifrangis M, Amerasinghe PH, Konradsen F, , 2010. Pre-elimination stage of malaria in Sri Lanka: assessing the level of hidden parasites in the population. Malar J 9: 16.[Crossref] [Google Scholar]
  8. Russell B, Suwanarusk R, Lek-Uthai U, , 2006. Plasmodium vivax genetic diversity: microsatellite length matters. Trends Parasitol 22: 399401.[Crossref] [Google Scholar]
  9. Cole-Tobian JL, Biasor M, King CL, , 2005. High complexity of Plasmodium vivax infections in Papua New Guinean children. Am J Trop Med Hyg 73: 626633. [Google Scholar]
  10. Cui L, Mascorro CN, Fan Q, Rzomp KA, Khuntirat B, Zhou G, Chen H, Yan G, Sattabongkot J, , 2003. Genetic diversity and multiple infections of Plasmodium vivax malaria in Western Thailand. Am J Trop Med Hyg 68: 613619. [Google Scholar]
  11. Joshi H, , 2003. Markers for population genetic analysis of human plasmodia species, P. falciparum and P. vivax . J Vector Borne Dis 40: 7883. [Google Scholar]
  12. Kim JR, Imwong M, Nandy A, Chotivanich K, Nontprasert A, Tonomsing N, Maji A, Addy M, Day NP, White NJ, Pukrittayakamee S, , 2006. Genetic diversity of Plasmodium vivax in Kolkata, India. Malar J 5: 110.[Crossref] [Google Scholar]
  13. Zakeri S, Barjesteh H, Djadid ND, , 2006. Merozoite surface protein-3alpha is a reliable marker for population genetic analysis of Plasmodium vivax . Malar J 5: 16.[Crossref] [Google Scholar]
  14. Bruce MC, Galinski MR, Barnwell JW, Snounou G, Day KP, , 1999. Polymorphism at the merozoite surface protein-3alpha locus of Plasmodium vivax: global and local diversity. Am J Trop Med Hyg 61: 518525. [Google Scholar]
  15. Galinski MR, Corredor-Medina C, Povoa M, Crosby J, Ingravallo P, Barnwell JW, , 1999. Plasmodium vivax merozoite surface protein-3 contains coiled-coil motifs in an alanine-rich central domain. Mol Biochem Parasitol 101: 131147.[Crossref] [Google Scholar]
  16. Galinski MR, Ingravallo P, Corredor-Medina C, Al-Khedery B, Povoa M, Barnwell JW, , 2001. Plasmodium vivax merozoite surface proteins-3beta and-3gamma share structural similarities with P. vivax merozoite surface protein-3alpha and define a new gene family. Mol Biochem Parasitol 115: 4153.[Crossref] [Google Scholar]
  17. Rayner JC, Corredor V, Feldman D, Ingravallo P, Iderabdullah F, Galinski MR, Barnwell JW, , 2002. Extensive polymorphism in the Plasmodium vivax merozoite surface coat protein MSP-3alpha is limited to specific domains. Parasitology 125: 393405.[Crossref] [Google Scholar]
  18. Ord R, Polley S, Tami A, Sutherland CJ, , 2005. High sequence diversity and evidence of balancing selection in the Pvmsp3alpha gene of Plasmodium vivax in the Venezuelan Amazon. Mol Biochem Parasitol 144: 8693.[Crossref] [Google Scholar]
  19. Veron V, Legrand E, Yrinesi J, Volney B, Simon S, Carme B, , 2009. Genetic diversity of msp3alpha and msp1_b5 markers of Plasmodium vivax in French Guiana. Malar J 8: 18.[Crossref] [Google Scholar]
  20. Imwong M, Nair S, Pukrittayakamee S, Sudimack D, Williams JT, Mayxay M, Newton PN, Kim JR, Nandy A, Osorio L, Carlton JM, White NJ, Day NP, Anderson TJ, , 2007. Contrasting genetic structure in Plasmodium vivax populations from Asia and South America. Int J Parasitol 37: 10131022.[Crossref] [Google Scholar]
  21. Koepfli C, Mueller I, Marfurt J, Goroti M, Sie A, Oa O, Genton B, Beck HP, Felger I, , 2009. Evaluation of Plasmodium vivax genotyping markers for molecular monitoring in clinical trials. J Infect Dis 199: 10741080.[Crossref] [Google Scholar]
  22. Schousboe ML, Rajakaruna RS, Salanti A, Hapuarachchi HC, Galappaththy GN, Bygbjerg IC, Amerasinghe PH, Konradsen F, Alifrangis M, , 2007. Island-wide diversity in single nucleotide polymorphisms of the Plasmodium vivax dihydrofolate reductase and dihydropteroate synthetase genes in Sri Lanka. Malar J 6: 16.[Crossref] [Google Scholar]
  23. Zubair L, Connor SJ, Ward N, Galapaththy GN, Mahanama S, Faizal HM, Yahiya Z, Sirwardhana M, Tennakoon U, Chandimala J, Razick S, Nawarathna B, Shaman J, Ndiaye O, Yang H, , 2007. Analysis of impacts of climate variability on malaria transmission and the development of an early warning system. Project Report to NOAA, NSF, EPA and EPRI Joint Program on Climate Variability and Human Health, December 2007. [Google Scholar]
  24. Konradsen F, Amerasinghe PH, Perera D, van der Hoek W, Amerasinghe FP, , 2000. A village treatment center for malaria: community response in Sri Lanka. Soc Sci Med 50: 879889.[Crossref] [Google Scholar]
  25. Briet OJ, Gunawardena DM, van der Hoek W, Amerasinghe FP, , 2003. Sri Lanka malaria maps. Malar J 2: 110.[Crossref] [Google Scholar]
  26. Excoffier L, Laval G, Schneider S, , 2005. Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1: 4750. [Google Scholar]
  27. Goudet J, , 2001. FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available at: http://www.unil.ch/izea/softwares/fstat.html. [Google Scholar]
  28. Park SD, , 2001. Trypanotolerance in West African cattle and the population genetic effects of selection. Ph.D. thesis, University of Dublin, Dublin, Ireland. [Google Scholar]
  29. Google, 2011. Google Earth. [Google Scholar]
  30. Wickramarachchi T, Premaratne PH, Dias S, Handunnetti SM, Udagama-Randeniya PV, , 2010. Genetic complexity of Plasmodium vivax infections in Sri Lanka, as reflected at the merozoite-surface-protein-3alpha locus. Ann Trop Med Parasitol 104: 95108.[Crossref] [Google Scholar]
  31. Bruce MC, Galinski MR, Barnwell JW, Donnelly CA, Walmsley M, Alpers MP, Walliker D, Day KP, , 2000. Genetic diversity and dynamics of Plasmodium falciparum and P. vivax populations in multiply infected children with asymptomatic malaria infections in Papua New Guinea. Parasitology 121: 257272.[Crossref] [Google Scholar]
  32. Mueller I, Kaiok J, Reeder JC, Cortes A, , 2002. The population structure of Plasmodium falciparum and Plasmodium vivax during an epidemic of malaria in the Eastern Highlands of Papua New Guinea. Am J Trop Med Hyg 67: 459464. [Google Scholar]
  33. Gunawardena S, Karunaweera ND, Ferreira MU, Phone-Kyaw M, Pollack RJ, Alifrangis M, Rajakaruna RS, Konradsen F, Amerasinghe PH, Schousboe ML, Galappaththy GNL, Abeyasinghe RR, Hartl DH, Wirth DF, , 2010. Geographic structure of Plasmodium vivax: microsatellite analysis of parasite populations from Sri Lanka, Myanmar and Ethiopia. Am J Trop Med Hyg 82: 235242.[Crossref] [Google Scholar]
  34. Dias S, Longacre S, Escalante AA, Udagama-Randeniya PV, , 2011. Genetic diversity and recombination at the C-terminal fragment of the merozoite surface protein-1 of Plasmodium vivax (PvMSP-1) in Sri Lanka. Infect Genet Evol 11: 145156.[Crossref] [Google Scholar]
  35. Anderson TJ, Haubold B, Williams JT, Estrada-Franco JG, Richardson L, Mollinedo R, Bockarie M, Mokili J, Mharakurwa S, French N, Whitworth J, Velez ID, Brockman AH, Nosten F, Ferreira MU, Day KP, , 2000. Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum . Mol Biol Evol 17: 14671482.[Crossref] [Google Scholar]
  36. Babiker HA, Lines J, Hill WG, Walliker D, , 1997. Population structure of Plasmodium falciparum in villages with different malaria endemicity in east Africa. Am J Trop Med Hyg 56: 141147. [Google Scholar]
  37. Imwong M, Snounou G, Pukrittayakamee S, Tanomsing N, Kim JR, Nandy A, Guthmann JP, Nosten F, Carlton J, Looareesuwan S, Nair S, Sudimack D, Day NP, Anderson TJ, White NJ, , 2007. Relapses of Plasmodium vivax infection usually result from activation of heterologous hypnozoites. J Infect Dis 195: 927933.[Crossref] [Google Scholar]

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  • Received : 27 May 2011
  • Accepted : 12 Sep 2011
  • Published online : 01 Dec 2011

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