1921
Volume 85, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Schistosomiasis continues to be a public health problem in many tropical and subtropical countries. Improving the diagnostic tools for surveillance and monitoring in areas that have reached elimination level will help hasten the possible elimination of this disease. This study therefore aims to develop enzyme-linked immunosorbent assay through the use of recombinant proteins such as thioredoxin peroxidase-1 (SjTPx-1) and four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, and Sj7TR). Cutoff values were calculated using 38 serum samples from healthy Japanese volunteers. Sera from 35 schistosomiasis-confirmed patients, four cured from the disease by chemotherapy, and 15 endemic negative controls were used to assess these antigens. SjTPx-1 and Sj7TR both had 85.71% sensitivity. Furthermore, these antigens were also tested against human sera positive for other parasitic infections and showed no or very minimal cross-reaction. These results suggest the potential defined antigens for development of an accurate diagnostic test for schistosomiasis.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2011.11-0245
2011-10-01
2017-11-24
Loading full text...

Full text loading...

/deliver/fulltext/14761645/85/4/674.html?itemId=/content/journals/10.4269/ajtmh.2011.11-0245&mimeType=html&fmt=ahah

References

  1. Kloos H, David R, , 2002. The paleoepidemiology of schistosomiasis in ancient Egypt. Hum Ecol Rev 9: 1425.
  2. WHO Fact Sheet on Schistosomiasis, 2010. Schistosomiasis. Available at: http://www.who.int/mediacentre/factsheets/fs115/en/index.html#. Accessed February 2, 2011.
  3. Olveda RM, Mahmoud A, , 2001. Disease in schistosomiasis japonica. , ed. Schistosomiasis. London, UK: Imperial College Press, 361385.[Crossref]
  4. Zhang YY, Luo JP, Liu YM, Wang QZ, Chen JH, Xu MX, Xu JM, Wu J, Tu XM, Wu GL, Zhang ZS, Wu HW, , 2009. Evaluation of Kato-Katz examination method in three areas with low-level endemicity of schistosomiasis japonica in China: a Bayesian modeling approach. Acta Trop 112: 1622.[Crossref]
  5. Lier T, Simonsen GS, Wang T, Lu D, Haukland HH, Vennervald BJ, Johansen MV, , 2009. Low sensitivity of the formol-ethyl acetate sedimentation concentration technique in low-intensity Schistosoma japonicum infections. PLoS Negl Trop Dis 3: e386.[Crossref]
  6. Yu JM, de Vlas SJ, Jiang QW, Gryseels B, , 2007. Comparison of the Kato-Katz technique, hatching test and indirect hemagglutination assay (IHA) for the diagnosis of Schistosoma japonicum infection in China. Parasitol Int 57: 4549.[Crossref]
  7. Doenhoff MJ, Chiodini PL, Hamilton JV, , 2004. Specific and sensitive diagnosis of schistosome infection: can it be done with antibodies? Trends Parasitol 20: 3539.[Crossref]
  8. Wu G, , 2002. A historical perspective on the immunodiagnosis of schistosomiasis in China. Acta Trop 82: 193198.[Crossref]
  9. Zhou Y, Zheng H, Chen Y, Zhang L, Wang K, Guo J, Huang Z, Zhang B, Huang W, Jin K, Dou T, Hasegawa M, Wang L, Zhang Y, Zhou J, Tao L, Cao Z, Li Y, Vinar T, Brejova B, Brown D, Li M, Miller DJ, Blair D, Zhong Y, Chen Z, Liu F, Wei H, Wang ZQ, Zhang QH, Song HD, Chen S, Xu X, Xu B, Ju C, Huang Y, Brindley PJ, McManus DP, Feng Z, Han ZG, Lu G, Ren S, Wang Y, Gu W, Kang H, Chen J, Chen X, Chen S, Wang L, Yan J, Wang B, Lu X, Jin L, Wang B, Pu S, Zhang X, Zhang W, Hu Q, Zhu G, Wang J, Yang H, Ning Z, Beriman M, Wei CL, Ruan Y, Zhao G, Wang S, , 2009. The Schistosoma japonicum genome reveals features of host-parasite interplay. Nature 460: 345356.[Crossref]
  10. Dzik J, , 2006. Molecules released by helminth parasites involved in host colonization. Acta Biochim Pol 53: 3364.
  11. Chiumento L, Bruschi F, , 2009. Enzymatic antioxidant systems in helminth parasites. Parasitol Res 105: 593603.[Crossref]
  12. Kang SW, Baines IC, Rhee SG, , 1998. Characterization of mammalian peroxiredoxin that contains one conserved cysteine. J Biol Chem 273: 63036311.[Crossref]
  13. Kumagai T, Osada Y, Kanazawa T, , 2006. 2-Cys peroxiredoxins from Schistosoma japonicum: the expression profile and localization in the life cycle. Mol Biochem Parasitol 149: 135143.[Crossref]
  14. Mulvenna J, Moertel L, Jones MK, Nawaratna S, Lovas EM, Gobert GN, Colgrave M, Jones A, Loukas A, McManus DP, , 2010. Exposed proteins of the Schistosoma japonicum tegument. Int J Parasitol 40: 543554.[Crossref]
  15. Jin Y, Lu K, Zhou WF, Fu ZQ, Liu JM, Shi YJ, Li H, Lin JJ, , 2010. Comparison of recombinant proteins from Schistosoma japonicum for schistosomiasis diagnosis. Clin Vaccine Immunol 17: 476480.[Crossref]
  16. Goto Y, Carter D, Gedurian J, Inoue N, Kawazu S, Reed SG, , 2010. Upregulated expression of B-cell antigen family tandem repeat proteins by Leishmania amastigotes. Infect Immun 78: 21382145.[Crossref]
  17. Kim TY, Kang SY, Ahn IY, Cho SY, Hong SJ, , 2001. Molecular cloning and characterization of an antigenic protein with a repeating region from Clonorchis sinensis . Korean J Parasitol 39: 5766.[Crossref]
  18. Goto Y, Coler RN, Reed SG, , 2007. Bioinformatic identification of tandem repeat antigens of the Leishmania donovani complex. Infect Immun 75: 846851.[Crossref]
  19. Goto Y, Duthie MS, Kawazu S, Inoue N, Carter D, , 2011. Biased cellular locations of tandem repeat antigens in African trypanosomes. Biochem Biophys Res Commun 405: 434438.[Crossref]
  20. Goto Y, Carter D, Reed SG, , 2008. Immunological dominance of Trypanosoma cruzi tandem repeat proteins. Infect Immun 76: 39673974.[Crossref]
  21. Kirinoki M, Hu M, Yokoi H, Chigusa Y, Matsuda H, , 2003. Immunoblot analysis of Schistosoma japonicum egg antigens with sera from patients with acute and chronic schistosomiasis japonica. Southeast Asian J Trop Med Public Health 34: 702707.
  22. Kirinoki M, Chigusa Y, Ohmae H, Sinuon M, Socheat D, Matsumoto J, Kitikoon V, Matsuda H, , 2011. Efficacy of sodium metaperiodate (SMP)-ELISA for the serodiagnosis of schistosomiasis mekongi. Southeast Asian J Trop Med Public Health 42: 2533.
  23. Nara T, Iizumi K, Ohmae H, Sy OS, Tsubota S, Inaba Y, Tsubouchi A, Tanabe M, Kojima S, Aoki T, , 2007. Antibody isotype responses to paramyosin, a vaccine candidate for schistosomiasis, and their correlations with resistance and fibrosis in patients infected with Schistosoma japonicum in Leyte, The Philippines. Am J Trop Med Hyg 76: 384391.
  24. Hertz-Fowler C, Peacock CS, Wood V, Aslett M, Kerhornou A, Mooney P, Tivey A, Berriman M, Hall N, Rutherford K, Parkhill J, Ivens AC, Rajandream MA, Barrell B, , 2004. GeneDB: a resource for prokaryotic and eukaryotic organisms. Nucleic Acids Res 32: D339D343.[Crossref]
  25. Benson G, , 1999. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res 27: 573580.[Crossref]
  26. Liu F, Lu J, Hu W, Wang SY, Cui SJ, Chi M, Yan Q, Wang XR, Song HD, Xu XN, Wang JJ, Zhang XL, Zhang X, Wang ZQ, Xue CL, Brindley PJ, McManus DP, Yang PY, Feng Z, Chen Z, Han ZG, , 2006. New perspective on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum . PLoS Pathog 2: e29.[Crossref]
  27. Wellcome Trust Sanger Institute, Pathogen Sequencing Unit, GeneDB. http:/www.genedb.org/. Accessed July 13, 2010.
  28. Matsuda H, Tanaka H, Blas BL, Nosenas JS, Tokawa T, Ohsawa S, , 1984. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med 54: 131138.
  29. Altman DG, , 1991. Inter-rater agreement. Practical Statistics for Medical Research. London, UK: Chapman and Hall, 404.
  30. Goto Y, Coler RN, Guderian J, Mohamath R, Reed SG, , 2006. Cloning, characterization, and serodiagnostic evaluation of Leishmania infantum tandem repeat proteins. Infect Immun 74: 39393945.[Crossref]
  31. Tan JC, Tan A, Checkley L, Honsa CM, Ferdig MT, , 2010. Variable numbers of tandem repeats in Plasmodium falciparum genes. J Mol Evol 71: 268278.[Crossref]
  32. Cheng PC, Tsaihong JC, Lee KM, , 2007. Application of recombinant Sjc26GST for serodiagnosis of Schistosoma japonicum infection in water buffalo (Bos buffelus). Vet Parasitol 150: 314320.[Crossref]
  33. Peng SY, Lee KM, Tsaihong JC, Cheng PC, Fan PC, , 2008. Evaluation of recombinant fructose-1,6-biphosphate aldolase ELISA test for the diagnosis of Schistosoma japonicum in water buffaloes. Res Vet Sci 85: 527533.[Crossref]
  34. Zhou XH, Wu JY, Huang XQ, Kunnon SP, Zhu XQ, Chen XG, , 2010. Identification and characterization of Schistosoma japonicum Sjp40, a potential antigen candidate for the early diagnosis of schistosomiasis. Diagn Microbiol Infect Dis 67: 337345.[Crossref]
  35. Boarino A, Scalone A, Gradoni L, Ferroglio E, Vitale F, Zanatta R, Giuffrida MG, Rosati S, , 2005. Development of recombinant chimeric antigen expressing immunodominant B epitopes of Leishmania infantum for serodiagnosis of visceral leishmaniasis. Clin Diagn Lab Immunol 12: 647653.
  36. Beghetto E, Spadoni A, Bruno L, Buffolano W, Gargano N, , 2006. Chimeric antigens of Toxoplasma gondii: toward standardization of toxoplasmosis serodiagnosis using recombinant products. J Clin Microbiol 44: 21332140.[Crossref]
  37. Goto Y, Howard RF, Bhatia A, Trigo J, Nakatani M, Netto EM, Reed SG, , 2009. Distinct antigen recognition pattern during zoonotic visceral leishmaniasis in humans and dogs. Vet Parasitol 160: 215220.[Crossref]
  38. Frank SA, , 2002. Immunological variability of hosts. Immunology and Evolution of Infectious Disease. Princeton, NJ: Princeton University Press, 132135.
  39. Xu J, Peeling RW, Chen JX, Wu XH, Wu ZD, Wang SP, Feng T, Chen SH, Li H, Guo JG, Zhou XN, , 2011. Evaluation of immunoassays for the diagnosis of Schistosoma japonicum infection using archived sera. PLoS Negl Trop Dis 5: e949.[Crossref]
  40. Leonardo LR, Acosta LP, Olveda RM, Aligui GDL, , 2002. Difficulties and strategies in the control of schistosomiasis in the Philippines. Acta Trop 82: 295299.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2011.11-0245
Loading
/content/journals/10.4269/ajtmh.2011.11-0245
Loading

Data & Media loading...

  • Received : 20 Apr 2011
  • Accepted : 21 Jul 2011

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error