1921
Volume 85, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The aim of this study was to investigate the association between genetic polymorphisms of chloroquine resistance transporter (), multidrug resistance 1 (), and ATPase () and clinical outcome after a three-day mefloquine-artesunate combination therapy in 134 patients with uncomplicated malaria in an area with multidrug resistance along the Thailand-Myanmar border. Analysis of gene mutation and amplification were performed by nested real-time polymerase chain reaction and SYBR Green I real-time polymerase chain reaction, respectively. The mutation for (codons 76, 220, 271, 326, 356, and 371) was found in all isolates (100%), whereas no mutation of (codon 86) and (codons 37, 693, 769, 898) was found. The copy number was significantly higher in isolates with recrudescence (median number = 2.44) compared with a sensitive response (median number = 1.44). The gene copy number was also found to be significantly higher in paired isolates collected before treatment and at the time of recrudescence. All isolates carried one gene copy.

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2011-09-01
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  • Received : 04 Apr 2011
  • Accepted : 13 Jun 2011

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