1921
Volume 85, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Resistance to chloroquine is a public health problem worldwide. Polymorphisms of the chloroquine resistance transporter () and multidrug resistance 1 () genes have been linked to chloroquine resistance. Although the K76T mutation in the gene has been shown to be a key determinant in chloroquine resistance, evidence suggests that the gene could modulate the level of chloroquine resistance. However, few studies of field isolates could identify the interactive role of these two genes in chloroquine resistance. Thus, we evaluated the influence of and polymorphisms on chloroquine sensitivity in 89 adapted isolates of from Thailand. We found that 87 of 89 isolates contained the CVIET haplotype of the gene. Two additional mutations in the gene were identified, i.e., K6Q and H97L. For the polymorphisms, the 184F allele was common in the parasites isolated along the Thailand-Cambodia border, and those isolated along the Thailand-Myanmar border contained higher copy numbers. Our results indicate that the additional mutations, in particular H97L in the gene and Y184F in the gene and its copy number, influence the level of chloroquine resistance.

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  • Received : 21 Feb 2011
  • Accepted : 24 Jun 2011

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