Volume 85, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A recent study by our group reported the isolation and partial serological and molecular characterization of four serogroup Ballum strains. Here, we reproduced experimental leptospirosis in golden Syrian hamsters () and carried out standardization of lethal dose 50% (LD50) of one of these strains (4E). Clinical disease features and histopathologic analyses of tissue lesions were also observed. As results, strain 4E induced lethality in the hamster model with inocula lower than 10 leptospires, and histopathological examination of animals showed typical lesions found in severe leptospirosis. Gross pathological findings were peculiar; animals that died early had more chance of presenting severe jaundice and less chance of presenting pulmonary hemorrhages ( < 0.01). serogroup Ballum has had a considerable growth in human leptospirosis cases in recent years. This strain has now been thoroughly characterized and can be used in more studies, especially evaluations of vaccine candidates.


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  1. Ricaldi JN, Vinetz JM, , 2006. Leptospirosis in the tropics and in travelers. Curr Infect Dis Rep 8: 5158.[Crossref] [Google Scholar]
  2. Ko AI, Goarant C, Picardeau M, , 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 7: 736747.[Crossref] [Google Scholar]
  3. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, , 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757771.[Crossref] [Google Scholar]
  4. Gouveia EL, Metcalfe J, de Carvalho AL, Aires TS, Villasboas-Bisneto JC, Queirroz A, Santos AC, Salgado K, Reis MG, Ko AI, , 2008. Leptospirosis-associated severe pulmonary hemorrhagic syndrome, Salvador, Brazil. Emerg Infect Dis 14: 505508.[Crossref] [Google Scholar]
  5. Arean VM, , 1962. Studies on the pathogenesis of leptospirosis. II. A clinicopathologic evaluation of hepatic and renal function in experimental leptospiral infections. Lab Invest 11: 273288. [Google Scholar]
  6. Silva ÉF, Santos CS, Athanazio DA, Seyffert N, Seixas FK, Cerqueira GM, Fagundes MQ, Brod CS, Reis MG, Dellagostin OA, Ko AI, , 2008. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine 26: 38923896.[Crossref] [Google Scholar]
  7. Sanger VL, Hamdy AH, Fizette WB, Bohl EH, Ferguson LC, , 1961. Leptospira pomona infection in hamsters. Cornell Vet 51: 489498. [Google Scholar]
  8. Badiola J, Thiermann AB, Cheville NF, , 1983. Pathologic features of leptospirosis in hamsters caused by Leptospira interrogans serovars hardjo and szwajizak. Am J Vet Res 44: 9199. [Google Scholar]
  9. Barnett JK, Barnett D, Bolin CA, Summers TA, Wagar EA, Cheville NF, Hartskeerl RA, Haake DA, , 1999. Expression and distribution of leptospiral outer membrane components during renal infection of hamsters. Infect Immun 67: 853861. [Google Scholar]
  10. Slack AT, Symonds ML, Dohnt MF, Craig SB, Smythe LD, , 2010. Molecular Epidemiology of Leptospira borgpetersenii serovar Arborea, Queensland, Australia, 1998–2005. Am J Trop Med Hyg 83: 820821.[Crossref] [Google Scholar]
  11. Herrmann Storck C, Postic D, Lamaury I, Perez JM, , 2008. Changes in epidemiology of leptospirosis in 2003–2004, a two El Niño Southern Oscillation period, Guadeloupe archipelago, French West Indies. Epidemiol Infect 136: 14071415. [Google Scholar]
  12. Thornley CN, Baker MG, Weinstein P, Maas EW, , 2002. Changing epidemiology of human leptospirosis in New Zealand. Epidemiol Infect 128: 2936.[Crossref] [Google Scholar]
  13. Vieira ML, Gama-Simões MJ, Collares-Pereira M, , 2006. Human leptospirosis in Portugal: a retrospective study of eighteen years. Int J Infect Dis 10: 378386.[Crossref] [Google Scholar]
  14. Silva ÉF, Felix SR, Cerqueira GM, Fagundes MQ, Seixas Neto ACP, Grassmann AA, Amaral MG, Gallina T, Dellagostin OA, , 2010. Preliminary characterization of Mus musculus-derived pathogenic strains of Leptospira borgpetersenii serogroup Ballum in a hamster model. Am J Trop Med Hyg 83: 336337.[Crossref] [Google Scholar]
  15. Faine SB, Adler B, Bolin C, Perolat P, , 1999. Leptospira and Leptospirosis. Melbourne, Australia: MediSci. [Google Scholar]
  16. Reed LJ, Muench H, , 1938. A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493497. [Google Scholar]
  17. Sonrier C, Branger C, Michel V, Ruvoën-Clouet N, Ganière JP, André-Fontaine G, , 2000. Evidence of cross-protection within Leptospira interrogans in an experimental model. Vaccine 19: 8694.[Crossref] [Google Scholar]
  18. Wang Z, Jin L, Wegrzyn A, , 2007. Leptospirosis vaccines. Microb Cell Fact 6: 3949.[Crossref] [Google Scholar]
  19. Palaniappan RUM, McDonough SP, Divers TJ, Chen CS, Pan MJ, Matsumoto M, Chang YF, , 2006. Immunoprotection of recombinant Leptospiral immunoglobulin-like protein a against Leptospira interrogans serovar pomona infection. Infect Immun 74: 17451750.[Crossref] [Google Scholar]
  20. Chang YF, Chen CS, Palaniappan RUM, He H, McDonough SP, Barr SC, Yan W, Faisal SM, Pan MJ, Chang CF, , 2007. Immunogenicity of the recombinant leptospiral putative outer membrane proteins as vaccine candidates. Vaccine 25: 81908197.[Crossref] [Google Scholar]
  21. Silva ÉF, Medeiros MA, McBride AJA, Matsunaga J, Esteves GS, Ramos JGR, Santos CS, Croda J, Homma A, Dellagostin OA, Haake DA, Reis MG, Ko AI, , 2007. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine 25: 62776286.[Crossref] [Google Scholar]
  22. Faisal SM, Yan W, Chen C, Palaniappan RUM, McDonough SP, Chang YF, , 2008. Evaluation of protective immunity of Leptospira immunoglobulin like protein A (LigA) DNA vaccine against challenge in hamsters. Vaccine 26: 277287.[Crossref] [Google Scholar]
  23. Suepaul SM, Carrington CV, Campbell M, Borde G, Adesiyun AA, , 2010. Study on the efficacy of Leptospira vaccines developed from serovars isolated from Trinidad and comparison with commercial vaccines using a hamster model. Vaccine 28: 54215426.[Crossref] [Google Scholar]
  24. Felix SR, Silva ÉF, Jouglard SDD, Hartmann DM, Grassmann AA, Dellagostin OA, , 2009. Leptospirosis vaccine: search for subunit candidates. Proc Vaccinol 1: 110114.[Crossref] [Google Scholar]
  25. Santos CS, Macedo JO, Bandeira M, Chagas-Junior AD, McBride AJ, McBride FW, Reis MG, Athanazio DA, , 2010. Different outcomes of experimental leptospiral infection in mouse strains with distinct genotypes. J Med Microbiol 59: 11011106.[Crossref] [Google Scholar]
  26. Bulach DM, Zuerner RL, Wilson P, Seemann T, McGrath A, Cullen PA, Davis J, Johnson M, Kuczek E, Alt DP, Peterson-Burch B, Coppel RL, Rood JI, Davies JK, Adler B, , 2006. Genome reduction in Leptospira borgpetersenii reflects limited transmission potential. Proc Natl Acad Sci USA 103: 1456014565.[Crossref] [Google Scholar]
  27. Nascimento ALTO, Ko AI, Martins EAL, Monteiro-Vitorello CB, Ho PL, Haake DA, , 2004. Comparative genomics of two Leptospira interrogans serovars reveals novel insights into physiology and pathogenesis. J Bacteriol 186: 21642172.[Crossref] [Google Scholar]
  28. Adler B, Moctezuma AD, , 2010. Leptospira and leptospirosis. Vet Microbiol 140: 287296.[Crossref] [Google Scholar]

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  • Received : 06 Jan 2011
  • Accepted : 25 Apr 2011
  • Published online : 01 Aug 2011

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