1921
Volume 84, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

remains an important cause of morbidity outside Africa, and no effective vaccine is available against this parasite. The Duffy binding protein (PvDBP) is essential during merozoite invasion into erythrocytes, and it is a target for protective immunity against malaria. This investigation was designed to evaluate naturally acquired antibodies to two variant forms of PvDBP-II antigen (DBP-I and -VI) in malaria individuals ( = 85; median = 22 years) who were living in hypoendemic areas in Iran. The two PvDBP-II variants were expressed in , and immunoglobulin G (IgG) isotype composition and avidity of naturally acquired antibodies to these antigens were measured using enzyme-linked immunosorbent assay (ELISA). Results showed that almost 32% of the studied individuals had positive antibody responses to the two PvDBP-II variants, and the prevalence of responders did not differ significantly ( > 0.05; χ test). The IgG-positive samples exhibited 37.03% and 40.8% high-avidity antibodies for PvDBP-I and PvDBP-VI variants, respectively. Furthermore, high-avidity IgG1 antibody was found in 39.1% of positive sera for each examined variant antigen. The avidity of antibodies for both PvDBP variant antigens and the prevalence of responders with high- and intermediate-avidity IgG, IgG1, and IgG3 antibodies were similar in patients ( > 0.05; χ test). Moreover, the prevalence of IgG antibody responses to the two variants significantly increased with exposure and host age. To sum up, the results provided additional data in our understanding of blood-stage immunity to PvDBP, supporting the rational development of an effective blood-stage vaccine based on this antigen.

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2011-06-01
2017-11-21
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References

  1. Mendis K, Sina BJ, Marchesini P, Carter R, , 2001. The neglected burden of Plasmodium vivax malaria. Am J Trop Med Hyg 64: 97106.
  2. Genton B, D'Acremont V, Rare L, Baea K, Reeder JC, Alpers MP, Muller I, , 2008. Plasmodium vivax and mixed infections are associated with severe malaria in children: a prospective cohort study from Papua New Guinea. PLoS Med 5: 881889.[Crossref]
  3. Tjitra E, Anstey NM, Sugiarto P, Warikar N, Kenangalem E, Karyana M, Lampah DA, Price RN, , 2008. Multidrug–resistant Plasmodium vivax associated with severe and fatal malaria: a prospective study in Papua, Indonesia. PLoS Med 5: 890899.[Crossref]
  4. Barnwell JW, Nichols ME, Rubinstein P, , 1989. In vitro evaluation of the role of the Duffy blood group in erythrocyte invasion by Plasmodium vivax . J Exp Med 169: 17951802.[Crossref]
  5. Adams JH, Sim BK, Dolan SA, Fang X, Kaslow DC, Miller LH, , 1992. A family of erythrocyte binding proteins of malaria parasites. Proc Natl Acad Sci USA 89: 70857089.[Crossref]
  6. Wertheimer SP, Barnwell JW, , 1989. Plasmodium vivax interaction with the human Duffy blood group glycoprotein: identification of a parasite receptor like protein. Exp Parasitol 69: 340350.[Crossref]
  7. Horuk R, Chitnis CE, Darbonne WC, Colby TJ, Rybicki A, Hadley TJ, Miller LH, , 1993. A receptor for the malarial parasite Plasmodium vivax. The erythrocyte chemokine receptor. Science 261: 11821184.[Crossref]
  8. Barnwell JW, Galinski MR, , 1995. Plasmodium vivax: a glimpse into the unique and shared biology of the merozoite. Ann Trop Med Parasitol 89: 113120.[Crossref]
  9. Ryan JR, Stoute JA, Amon J, Dunton RF, Mtalib R, Koros J, Owour B, Luckhart SH, Wirtz RA, Barnwell JW, Rosenberg R, , 2006. Evidence for transmission of Plasmodium vivax among a Duffy antigen negative population in western Kenya. Am J Trop Med Hyg 75: 575581.
  10. Menard D, Barnadas C, Bouchier C, Henry-Halldin C, Ratsimbasoa A, Thonier V, Carod JF, Domarle O, Colin Y, Bertrand O, Picot K, King CL, Grimberg BT, Mercereau-Puijalon O, Zimmerman PA, , 2010. Plasmodium vivax clinical malaria is commonly observed in Duffy-negative Malagasy people. Proc Natl Acad Sci USA 107: 59675971.[Crossref]
  11. Adam H, Hudson DE, Torii M, Ward GE, Wellems TE, Aikawa M, Miller LH, , 1990. The Duffy receptor family of Plasmodium knowlesi is located within the micronemes of invasive malaria merozoites. Cell 63: 141153.[Crossref]
  12. Chitnis CE, Miller LH, , 1994. Identification of the erythrocyte binding domains of Plasmodium vivax and Plasmodium knowlesi proteins involved in erythrocyte invasion. J Exp Med 180: 497506.[Crossref]
  13. Chitnis CE, Chaudhuri A, Horuk R, Pogo AO, Miller LH, , 1996. The domain on the Duffy blood group antigen for binding Plasmodium vivax and P. knowlesi malarial parasites to erythrocytes. J Exp Med 184: 15311536.[Crossref]
  14. Ranjan A, Chitnis CE, , 1999. Mapping regions containing binding residues within functional domains of Plasmodium vivax and Plasmodium knowlesi erythrocyte–binding proteins. Proc Natl Acad Sci USA 96: 1406714072.[Crossref]
  15. Singh SK, Singh AP, Pandey S, Yazdani SS, Chitnis CE, Sharma A, , 2003. Definition of structural elements in Plasmodium vivax and P. knowlesi Duffy–binding domains necessary for erythrocyte invasion. Biochem J 374: 193198.[Crossref]
  16. Tsuboi T, Kappe SH, Al-Yaman F, Prickett MD, Alpers M, Adams JH, , 1994. Natural variation within the principal adhesion domain of the Plasmodium vivax Duffy binding protein. Infect Immun 62: 55815586.
  17. Ampudia E, Patarroyo MA, Patarroyo ME, Murillo LA, , 1996. Genetic polymorphism of the Duffy receptor binding domain of Plasmodium vivax in Colombian wild isolates. Mol Biochem Parasitol 78: 269272.[Crossref]
  18. Xainli J, Adams JH, King CL, , 2000. The erythrocyte binding motif of Plasmodium vivax Duffy binding protein is highly polymorphic and functionally conserved in isolates from Papua New Guinea. Mol Biochem Parasitol 111: 253260.[Crossref]
  19. Cole-Tobian J, King CL, , 2003. Diversity and natural selection in Plasmodium vivax Duffy binding protein gene. Mol Biochem Parasitol 127: 121132.[Crossref]
  20. Babaeekho L, Zakeri S, Djadid ND, , 2009. Genetic mapping of the Duffy binding protein (DBP) ligand domain of Plasmodium vivax from unstable malaria region in the Middle East. Am J Trop Med Hyg 80: 112118.
  21. Michon P, Fraser T, Adams JH, , 2000. Naturally acquired and vaccine-elicited antibodies block erythrocyte cytoadherence of the Plasmodium vivax Duffy binding protein. Infect Immun 68: 31643171.[Crossref]
  22. Cole-Tobian JL, Cortes A, Baisor M, Kastens W, Xainli J, Bockarie M, Adams JH, King CL, , 2002. Age-acquired immunity to a Plasmodium vivax invasion ligand, the Duffy binding protein. J Infect Dis 186: 531539.[Crossref]
  23. Xainli J, Cole-Tobian JL, Baisor M, Kastens W, Bockarie M, Yazdani SS, Chitnis CE, Adams JH, King CL, , 2003. Epitope-specific humoral immunity to Plasmodium vivax Duffy binding protein. Infect Immun 71: 25082515.[Crossref]
  24. Xainli J, Baiso M, Kastens W, Bockarie M, Adams JH, King CL, , 2002. Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans. J Immunol 169: 32003207.[Crossref]
  25. Cole-Tobian JL, Michon P, Biasor M, Richards JS, Beeson JG, Mueller I, King CL, , 2009. Strain-specific Duffy binding protein antibodies correlate with protection against infection with homologous compared to heterologous Plasmodiun vivax strains in Papua New Guinean children. Infect Immun 77: 40094017.[Crossref]
  26. Ceravolo IP, Souza-Silva FA, Fontes CJ, Braga EM, Madureira AP, Krettli AU, Souza JM, Brito CF, Adams JH, Carvalho LH, , 2008. Inhibitory properties of the antibody response to Plasmodium vivax Duffy binding protein in an area with unstable malaria transmission. Scand J Immunol 67: 270278.[Crossref]
  27. Ceravolo IP, Sanchez BA, Sousa TN, Guerra BM, Soares IS, Braga EM, McHenry AM, Adams JH, Brito CF, Carvalho LH, , 2009. Naturally acquired inhibitory antibodies to Plasmodium vivax Duffy binding protein are short-lived and allele-specific following a single malaria infection. Clin Exp Immunol 156: 502510.[Crossref]
  28. Souza-Silva FA, da Silva-Nunes M, Sanchez BA, Ceravolo IP, Malafronte RS, Brito CF, Ferreira MU, Carvalho LH, , 2010. Naturally acquired antibodies to Plasmodium vivax Duffy binding protein (DBP) in rural Brazilian Amazon. Am J Trop Med Hyg 82: 185193.[Crossref]
  29. Michon PA, Arevalo-Herrera M, Fraser T, Herrera S, Adams JH, , 1998. Serologic responses to recombinant Plasmodium vivax Duffy binding protein in a Colombian village. Am J Trop Med Hyg 59: 597599.
  30. Fraser T, Michon P, Barnwell JW, Noe AR, Al-Yaman F, Kaslow DC, Adams JH, , 1997. Expression and serologic activity of a soluble recombinant Plasmodium vivax Duffy binding protein. Infect Immun 65: 27722777.
  31. Singh S, Pandey K, Chattopadhayay R, Yazdani SS, Lynn A, Bharadwaj A, Ranjan A, Chitnis C, , 2001. Biochemical, biophysical, and functional characterization of bacterially expressed and refolded receptor binding domain of Plasmodium vivax Duffy-binding protein. J Biol Chem 276: 1711117116.[Crossref]
  32. Grimberg BT, Udomsangpetch R, Xainli J, McHenry A, Panichakul T, Sattabongkot J, Cui L, Bockarie M, Chitnis CE, Adams J, Zimmerman PA, King CHL, , 2007. Vivax invasion of human erythrocytes inhibited by antibodies directed against the Duffy binding protein. PLoS Med 4: 19401947.[Crossref]
  33. Ceravolo IP, Bruna-Romero O, Braga EM, Fontes CJ, Brito CF, Souza JM, Krettli AU, Adams JH, Carvalho LH, , 2005. Anti-Plasmodium vivax Duffy binding protein antibodies measure exposure to malaria in the Brazilian Amazon. Am Trop Med Hyg 72: 675681.
  34. Zakeri S, Najafabadi ST, Zare A, Djadid ND, , 2002. Detection of malaria parasites by nested PCR in south–eastern, Iran: evidence of highly mixed infections in Chahbahar district. Malar J 1: 2.[Crossref]
  35. Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, , 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315320.[Crossref]
  36. Hedman K, Lappalainen M, Seppaia I, Makela O, , 1989. Recent primary Toxoplasma infection indicated by a low avidity of specific IgG. J Infect Dis 159: 736740.[Crossref]
  37. Suárez-mutis MC, Cuervo P, Leoratti FM, Moraes-Avila SL, Ferreira AW, Fernandes O, Coura JR, , 2007. Cross sectional study reveals a high percentage of asymptomatic Plasmodium vivax infection in the Amazon Rio Negro area, Brazil Rev. Inst Med Trop Sao Paulo 49: 159164.[Crossref]
  38. Baum J, Thomas AW, Conway DJ, , 2003. Evidence for diversifying selection on erythrocyte-binding antigens of Plasmodium falciparum and P. vivax . Genetics 163: 13271336.
  39. Kho WG, Chung JY, Sim EJ, Kim DW, Chung WC, , 2001. Analysis of polymorphic regions of Plasmodium vivax Duffy binding protein of Korean isolates. Korean J Parasitol 39: 143150.[Crossref]
  40. Sousa TN, Ceravolo IP, Frnandes Fontes CJ, Couto A, Carvalho LH, Brito CF, , 2006. The pattern of major polymorphisms in the Duffy binding protein ligand domain from the Brazilian Amazon area. Mol Biochem Parasitol 146: 251254.[Crossref]
  41. Egan A, Waterfall M, Pinder M, Holder A, Riley E, , 1997. Characterization of human T- and B-cell epitopes in the C terminus of Plasmodium falciparum merozoite surface protein 1: evidence for poor T-cell recognition of polypeptides with numerous disulfide bonds. Infect Immun 65: 30243031.
  42. Quin SJ, Seixas EM, Cross CA, Berg M, Lindo V, Stockinger B, Langhorne J, , 2001. Low CD4+ T cell responses to the C-terminal region of the malaria merozoite surface protein-1 may be attributed to processing within distinct MHC class II pathways. Eur J Immunol 31: 7281.[Crossref]
  43. Ferreira MU, Kimura EA, Souza JM, Katzin AM, , 1996. The isotype composition and avidity of naturally acquired anti-Plasmodium falciparum antibodies: differential patterns in clinically immune Africans and Amazonians patients. Am J Trop Med Hyg 55: 315323.
  44. Soares IS, Barnwell JW, Ferreira MU, Gomes Da Cunha M, Laurino JP, Castilho BA, Rodrigues MM, , 1999. A Plasmodium vivax vaccine candidate displays limited allele polymorphism, which doses not restrict recognition by antibodies. Mol Med 5: 459470.
  45. Aikawa M, Miller LH, Johnson J, Rabbege J, , 1978. Erythrocyte entry by malaria parasites. A moving junction between erythrocyte and parasite. J Cell Biol 77: 7282.[Crossref]
  46. Cowman A, Crabb B, , 2006. Invasion of red blood cells by malaria parasites. Cell 124: 755766.[Crossref]
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  • Received : 01 Jan 2011
  • Accepted : 09 Mar 2011

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