Volume 85, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Previous studies of transmission to spp. mosquitoes have not been able to predict mosquito infectivity on the basis of microscopic or molecular quantification of parasites (total parasites in the sample or total number of gametocytes) in infected blood. Two methods for production of ookinete cultures , with yields of 10 macrogametocytes, 10 zygotes, and 10 ookinetes, respectively, per 10 mL of infected patient blood with approximately 0.01% parasitemia, were used to study sexual stage development. The quantity of gametocytes, determined by counting Giemsa-stained blood smears, and quantity and type of gametocyte as determined by quantitative reverse transcriptase–polymerase chain reaction for Pvalpha tubulin II and macrogametocyte-specific did not predict ookinete yield. Factors that affect the efficiency of ookinete transformation remain poorly understood.


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  • Received : 25 Dec 2010
  • Accepted : 17 May 2011
  • Published online : 01 Aug 2011

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