Volume 84, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Definitive diagnosis of infection in adult patients is a clinically important challenge. Chronically infected adults pass few eggs in the urine, which are often missed when current diagnostic methods are used. In the work presented here, we report on an alternative diagnostic method based on presence of the -specific 1, 121 bp repeat fragment in human urine. A novel method of collecting the urine specimens in the field and filtering them through heavy Whatman No. 3 paper was introduced. After drying, the samples remained viable for several months at room temperature. To test the potential use of this method, 89 urine specimens from school children in Kollo District, Niger, were examined. In all, 52 of 89 (58.4%) were positive for hematuria, 4 of 89 (49.4%) were positive for eggs, and 51 of 89 (57.3%) showed parasite-specific DNA. These were compared with 60 filtered urine specimens obtained from random samples of adults from two study sites in Nigeria, one endemic and one non-endemic for . In the 30 patients from the endemic site, all 10 samples with detectable eggs and 7 of the 20 egg-negative samples were DNA positive. It was concluded that the urine filter paper method was sufficiently sensitive to detect low and cryptic infections, that DNA detection was more sensitive than egg detection, and that the filtration method facilitated specimen collection and transport from the field.


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  1. Jordan P, Webbe G, , 1969. Human Schistosomiasis. Springfield, IL: Charles C. Thomas, 105115. [Google Scholar]
  2. Clarke VD, , 1966. The influence of acquired resistance in the epidemiology of bilharziasis. Cent Afr J Med 12: 130. [Google Scholar]
  3. Mostafa MH, Sheweita SA, O'Connor PJ, , 1999. Relationship between schistosomiasis and bladder cancer. Clin Microbiol Rev 12: 97111. [Google Scholar]
  4. Hotez PJ, Engels D, Fenwick A, Savioli L, , 2010. Africa is desperate for praziquantel. Lancet 376: 496498.[Crossref] [Google Scholar]
  5. Shiff C, Naples JM, Isharwal S, Bosompem KM, Veltri RW, , 2010. Non-invasive methods to detect schistosome-based bladder cancer: is the association sufficient for epidemiological use? Trans R Soc Trop Med Hyg 104: 35.[Crossref] [Google Scholar]
  6. Koukounari A, Webster JP, Donnelly CA, Bray BC, Naples J, Bosompem K, Shiff C, , 2009. Sensitivities and specificities of diagnostic tests and infection prevalence of Schistosoma haematobium estimated from data on adults in villages northwest of Accra, Ghana. Am J Trop Med Hyg 80: 435441. [Google Scholar]
  7. Mharakurwa S, Simoloka C, Thuma PE, Shiff CJ, Sullivan DJ, , 2006. PCR detection of Plasmodium falciparum in human urine and saliva samples. Malar J 5: 103.[Crossref] [Google Scholar]
  8. Pontes LA, Dias-Neto E, Rabello A, , 2002. Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces. Am J Trop Med Hyg 66: 157162. [Google Scholar]
  9. Hamburger J, Xu YX, Ramzy RM, Jourdane J, Ruppel A, , 1998. Development and laboratory evaluation of a polymerase chain reaction for monitoring Schistosoma mansoni infestation of water. Am J Trop Med Hyg 59: 468473. [Google Scholar]
  10. Obeng BB, Aryeetey YA, de Dood CJ, Amoah AS, Larbi IA, Deelder AM, Yazdanbakhsh M, Hartgers FC, Boakye DA, Verweij JJ, Van Dam GJ, Van Lieshout L, , 2008. Application of a circulating-cathodic-antigen (CCA) strip test and real-time PCR, in comparison with microscopy, for the detection of Schistosoma haematobium in urine samples from Ghana. Ann Trop Med Parasitol 102: 625633.[Crossref] [Google Scholar]
  11. Kjetland EF, Hove RJ, Gomo E, Midzi N, Gwanzura L, Mason P, Friis H, Verweij JJ, Gundersen SG, Ndhlovu PD, Mduluza T, Van Lieshout L, , 2009. Schistosomiasis PCR in vaginal lavage as an indicator of genital Schistosoma haematobium infection in rural Zimbabwean women. Am J Trop Med Hyg 81: 10501055.[Crossref] [Google Scholar]
  12. Lier T, Simonsen GS, Wang T, Lu D, Haukland HH, Vennervald BJ, Hegstad J, Johansen MV, , 2009. Real-time polymerase chain reaction for detection of low-intensity Schistosoma japonicum infections in China. Am J Trop Med Hyg 81: 428432. [Google Scholar]
  13. ten Hove RJ, Verweij JJ, Vereecken K, Polman K, Dieye L, Van Lieshout L, , 2008. Multiplex real-time PCR for the detection and quantification of Schistosoma mansoni and S. haematobium infection in stool samples collected in northern Senegal. Trans R Soc Trop Med Hyg 102: 179185.[Crossref] [Google Scholar]
  14. Hamburger J, He N, Abbasi I, Ramzy RM, Jourdane J, Ruppel A, , 2001. Polymerase chain reaction assay based on a highly repeated sequence of Schistosoma haematobium: a potential tool for monitoring schistosome-infested water. Am J Trop Med Hyg 65: 907911. [Google Scholar]
  15. Weber MD, Blair DM, Clark VV, , 1967. The pattern of schistosome egg distribution in a micturition flow. Cent Afr J Med 13: 7588. [Google Scholar]

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  • Received : 03 Dec 2010
  • Accepted : 07 Mar 2011
  • Published online : 01 Jun 2011

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