Volume 85, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Mosquitoes in the complex are important vectors of several disease-causing pathogens, including West Nile virus. In North America, the complex consists of . form pipiens, . form molestus, , and their hybrids that exhibit substantial diversity in physiology, behavior, and geographic range. Hybridization among these mosquitoes is of concern because of potential implications for disease transmission. Currently, several morphological and molecular markers exist for differentiating members of the complex; however, these markers have specific limitations. We report here two highly reliable ribosomal DNA-based single nucleotide polymorphism (SNP) markers, CxpG2T and CxpA2d, for detecting complex mosquitoes containing alleles. Both CxpG2T and CxpA2d contain one allele that is present in all members of the complex, and the other allele is specific to . Testing of field populations from the eastern United States showed that these two SNP markers are capable of identifying a south to north gradient of and hybrids. The northern limit of detection of alleles in this study was in Fort Totten, NY (40.79°N), whereas the southern boundary was determined between Atlanta, GA (33.81°N) and Gainesville, FL (29.64°N). CxpG2T and CxpA2d were more accurate than the marker, and they may conceivably provide comparable resolution with microsatellite markers for detecting alleles.


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  • Received : 29 Nov 2010
  • Accepted : 08 Jun 2011
  • Published online : 01 Sep 2011

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