1921
Volume 85, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

drug sensitivity and molecular analyses of track drug resistance. DNA-binding fluorescent dyes like SYBR Green I may allow field laboratories, proximal to collection sites, to conduct drug assays. In 2007–2008, we assayed 121 field isolates from western Kenya for 50% inhibitory concentrations (IC) against 6 antimalarial drugs using a SYBR Green I assay: 91 immediate (IEV) and 30 culture-adapted, along with reference clones D6 (chloroquine [CQ] sensitive) and W2 (CQ resistant). We also assessed () copy number and single nucleotide polymorphisms (SNPs) at four codons. The ICs for IEV and culture-adapted isolates were similar, and approximated historical ICs. For , mean copy number was 1, with SNPs common at codons 86 and 184. The SYBR Green I assay adapted well to our field-based laboratory, for both IEV and culture-adapted , warranting continued use.

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2017-09-21
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  • Received : 29 Nov 2010
  • Accepted : 11 Feb 2011

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