1921
Volume 85, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The presence of , and in 209 fleas () obtained from domestic cats and dogs in several locations in Malaysia was investigated in this study. Using a polymerase chain reaction specific for the citrate synthase () and 17-kD antigenic protein () genes of rickettsiae, we detected DNA in 6 (2.9%) fleas. For detection of bartonellae, amplification of the heme-binding protein () and riboflavin synthase () genes identified and DNA in 24 (11.5%) and 40 (19.1%) fleas, respectively. The DNA of and was detected in 10 (4.8%) fleas. Two genogroups (Marseille and Houston-1) were detected in this study; genogroup Marseille (genotype Fizz) was found more often in the fleas. The findings in this study suggest fleas as potential vectors of rickettsioses and cat-scratch disease in this country.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.2011.10-0634
2011-11-01
2020-07-05
Loading full text...

Full text loading...

/deliver/fulltext/14761645/85/5/931.html?itemId=/content/journals/10.4269/ajtmh.2011.10-0634&mimeType=html&fmt=ahah

References

  1. Traub R, Wisseman CL Jr, Azad AF, 1978. The ecology of murine typhus-a critical review. Trop Dis Bull 75: 237317.
    [Google Scholar]
  2. Azad AF, Radulovic S, Higgins JA, Noden BH, Troyer JM, 1997. Flea-borne rickettsioses: ecologic considerations. Emerg Infect Dis 3: 319327.[Crossref]
    [Google Scholar]
  3. Azad AF, Beard CB, 1998. Rickettsial pathogens and their arthropod vectors. Emerg Infect Dis 4: 179186.[Crossref]
    [Google Scholar]
  4. Reif KE, Macaluso KR, 2009. Ecology of Rickettsia felis: a review. J Med Entomol 46: 723736.[Crossref]
    [Google Scholar]
  5. Pérez-Osorio CE, Zavala-Velázquez JE, Arias León JJ, Zavala-Castro JE, 2008. Rickettsia felis as emergent global threat for humans. Emerg Infect Dis 14: 10191023.[Crossref]
    [Google Scholar]
  6. Abbot P, Aviles AE, Eller L, Durden LA, 2007. Mixed infections, cryptic diversity, and vector-borne pathogens: evidence from Polygenis fleas and Bartonella species. Appl Environ Microbiol 73: 60456052.[Crossref]
    [Google Scholar]
  7. Li W, Raoult D, Fournier PE, 2007. Genetic diversity of Bartonella henselae in human infection detected with multispacer typing. Emerg Infect Dis 13: 11781183.[Crossref]
    [Google Scholar]
  8. Zeaiter Z, Fournier PE, Raoult D, 2002. Genomic variation of Bartonella henselae strains detected in lymph nodes of patients with cat scratch disease. J Clin Microbiol 40: 10231030.[Crossref]
    [Google Scholar]
  9. Tay ST, Ho TM, Rohani MY, Devi S, 2000. Antibodies to Orientia tsutsugamushi, Rickettsia typhi and spotted fever group rickettsiae among febrile patients in rural areas of Malaysia. Trans R Soc Trop Med Hyg 94: 280284.[Crossref]
    [Google Scholar]
  10. Aleeksev AN, Dubinina HV, Van De Pol I, Schouls LM, 2001. Identification of Ehrlichia spp. and Borrelia burgdorferi in Ixodes ticks in the Baltic Regions of Russia. J Clin Microbiol 39: 22372242.[Crossref]
    [Google Scholar]
  11. Labruna MB, Whitworth T, Horta MC, Bouyer DH, McBride JW, Pinter A, Popov V, Gennari SM, Walker DH, 2004. Rickettsia species infecting Amblyomma cooperi ticks from an endemic area for Brazilian spotted fever in the state of Sao Paolo, Brazil. J Clin Microbiol 42: 9098.[Crossref]
    [Google Scholar]
  12. Carl M, Tibbs CW, Dobson ME, Paparello S, Dasch GA, 1990. Diagnosis of acute typhus infection using polymerase chain reaction. J Infect Dis 161: 791793.[Crossref]
    [Google Scholar]
  13. Bereswill S, Hinkelmann S, Kist M, Sander A, 1999. Molecular analysis of riboflavin synthesis genes in Bartonella henselae and use of the ribC gene for differentiation of Bartonella species by PCR. J Clin Microbiol 37: 31593166.
    [Google Scholar]
  14. Loftis AD, Reeves WK, Szumlas DE, Abbassy MM, Helmy IM, Moriarty JR, Dasch GA, 2006. Surveillance of Egyptian fleas for agents of public health significance: Anaplasma, Bartonella, Coxiella, Ehrlichia, Rickettsia, and Yersinia pestis . Am J Trop Med Hyg 75: 4148.
    [Google Scholar]
  15. Bitam I, Rolain JM, Kernif T, Baziz B, Parola P, Raoult D, 2009. Bartonella species detected in rodents and hedgehogs from Algeria. Clin Microbiol Infect 2: 102103.[Crossref]
    [Google Scholar]
  16. Parola P, Sanogo OY, Lerdthusnee K, Zeaiter Z, Chauvancy G, Gonzalez JP, Miller RS, Telford III Sr, Wongsrichanalai C, Raoult D, 2003. Identification of Rickettsia spp. and Bartonella spp. in fleas from the Thai-Myanmar border. Ann N Y Acad Sci 990: 173181.[Crossref]
    [Google Scholar]
  17. Hornok S, Meli ML, Perreten A, Farkas R, Willi B, Beugnet F, Lutz H, Hofmann-Lehmann R, 2010. Molecular investigation of hard ticks (Acari: Ixodidae) and fleas (Siphonaptera: Pulicidae) as potential vectors of rickettsial and mycoplasmal agents. Vet Microbiol 140: 98104.[Crossref]
    [Google Scholar]
  18. Reeves WK, Nelder MP, Korecki JA, 2005. Bartonella and Rickettsia in fleas and lice from mammals in South Carolina, USA. J Vector Ecol 30: 310315.
    [Google Scholar]
  19. Forshey BM, Stewart A, Morrison AC, Gálvez H, Rocha C, Astete H, Eza D, Chen HW, Chao CC, Montgomery JM, Bentzel DE, Ching WM, Kochel TJ, 2010. Epidemiology of spotted fever group and typhus group rickettsial infection in the Amazon basin of Peru. Am J Trop Med Hyg 82: 683690.[Crossref]
    [Google Scholar]
  20. Venzal JM, Pérez-Martínez L, Félix ML, Portillo A, Blanco JR, Oteo JA, 2006. Prevalence of Rickettsia felis in Ctenocephalides felis and Ctenocephalides canis from Uruguay. Ann N Y Acad Sci 1078: 305308.[Crossref]
    [Google Scholar]
  21. Fang R, Raoult D, 2003. Antigenic classification of Rickettsia felis by using monoclonal and polyclonal antibodies. Clin Diagn Lab Immunol 10: 221228.
    [Google Scholar]
  22. Jiang J, Soeatmadji DW, Henry KM, Ratiwayanto S, Bangs MJ, Richards AL, 2006. Rickettsia felis in Xenopsylla cheopis, Java, Indonesia. Emerg Infect Dis 12: 12811283.[Crossref]
    [Google Scholar]
  23. Chomel BB, Carlos ET, Kasten RW, Yamamoto K, Chang CC, Carlos RS, Abenes MV, Pajares CM, 1999. Bartonella henselae and Bartonella clarridgeiae infection in domestic cats from the Philippines. Am J Trop Med Hyg 60: 593597.
    [Google Scholar]
  24. Bhengsri S, Baggett HC, Peruski LF Jr, Morway C, Bai Y, Fisk TL, Sitdhirasdr A, Maloney SA, Dowell SF, Kosoy M, 2010. Bartonella spp. infections, Thailand. Emerg Infect Dis 16: 743745.[Crossref]
    [Google Scholar]
  25. Maruyama S, Sakai T, Morita Y, Tanaka S, Kabeya H, Boonmar S, Poapolathep A, Chalarmchaikit T, Chang CC, Kasten RW, Chomel BB, Katsube Y, 2001. Prevalence of Bartonella species and 16s rRNA gene types of Bartonella henselae from domestic cats in Thailand. Am J Trop Med Hyg 65: 783787.
    [Google Scholar]
  26. Bergmans AM, De Jong CM, Van Amerongen G, Schot CS, Schouls LM, 1997. Prevalence of Bartonella species in domestic cats in The Netherlands. J Clin Microbiol 35: 22562261.
    [Google Scholar]
  27. Gurfield AN, Boulouis HJ, Chomel BB, Kasten RW, Heller R, Bouillin C, Gandoin C, Thibault D, Chang CC, Barrat F, Piemont Y, 2001. Epidemiology of Bartonella infection in domestic cats in France. Vet Microbiol 80: 185198.[Crossref]
    [Google Scholar]
  28. Sander A, Posselt M, Böhm N, Ruess M, Altwegg M, 1999. Detection of Bartonella henselae DNA by two different PCR assays and determination of the genotypes of strains involved in histologically defined cat scratch disease. J Clin Microbiol 37: 993997.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2011.10-0634
Loading
/content/journals/10.4269/ajtmh.2011.10-0634
Loading

Data & Media loading...

  • Received : 09 Nov 2010
  • Accepted : 02 Mar 2011
  • Published online : 01 Nov 2011
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error