Volume 84, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



This report presents three cases where the rK39 strip test failed to diagnose two cases of post–kala-azar dermal leishmaniasis and one case of visceral leishmaniasis. However, a strong clinical suspicion prompted further evaluation by polymerase chain reaction (PCR), which established the etiology. The present case series highlights the usefulness of PCR in the diagnosis of leishmaniasis.


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  2. Ganguly S, Das NK, Panja M, Pal S, Modak D, Rahaman M, Mallik S, Guha SK, Pramanik N, Goswami R, Barbhuiya JN, Saha B, Chatterjee M, , 2008. Increased levels of interleukin-10 and IgG3 are hallmarks of Indian post-kala-azar dermal leishmaniasis. J Infect Dis 197: 17621771.[Crossref]
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  6. Singh R, Subba Raju BV, Jain RK, Salotra P, , 2005. Potential of direct agglutination test based on promastigote and amastigote antigens for serodiagnosis of post-kala-azar dermal leishmaniasis. Clin Diagn Lab Immunol 12: 11911194.
  7. Chatterjee M, Basu K, Basu D, Bannerjee D, Pramanik N, Guha SK, Goswami RP, Saha SK, Mandal C, , 1998. Distribution of IgG subclasses in antimonial unresponsive Indian kala-azar patients. Clin Exp Immunol 114: 408413.[Crossref]
  8. Schönian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, Jaffe CL, , 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47: 349358.[Crossref]
  9. Sundar S, , 2001. Drug resistance in Indian visceral leishmaniasis. Trop Med Int Health 6: 849854. Erratum in: 2002 Trop Med Int Health 7: 293.[Crossref]
  10. Department of Health and Family Welfare, Government of West Bengal, 2009. Reported Cases and Deaths Due to Kala-azar in West Bengal 2002–2008. Available at: http://www.wbhealth.gov.in/Statistics%20_Html/2008_2009/9/9_15.htm. Accessed September 30, 2010.
  11. Ganguly S, Bandyopadhyay S, Sarkar A, Chatterjee M, , 2006. Development of a semi-automated colorimetric assay for screening anti-leishmanial agents. J Microbiol Methods 66: 7986.[Crossref]
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  13. Mondal D, Singh SP, Kumar N, Joshi A, Sundar S, Das P, Siddhivinayak H, Kroeger A, Boelaert M, , 2009. Visceral leishmaniasis elimination programme in India, Bangladesh, and Nepal: reshaping the case finding/case management strategy. PLoS Negl Trop Dis 3: e355.[Crossref]

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  • Received : 18 Oct 2010
  • Accepted : 17 Jan 2011

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