1921
Volume 84, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The technical capability of different methods to diagnose in maternal peripheral blood, placenta, and umbilical cord blood has not been assessed in Colombia and seldom explored in other malaria-endemic regions. We designed a study to compare the technical and the operational-economical performances of light microscopy (LM), nested polymerase chain reaction (nPCR), and histopathology (HP). In maternal blood, LM had 41% sensitivity and 100% specificity and in placental blood, 35% and 100%, respectively, compared with nPCR. In placental tissue, LM had 33% sensitivity and 95% specificity; and nPCR 47% and 77%, respectively; compared with HP. Light microscopy had the best operational-economical qualification. We concluded that nPCR and HP performed better compared with LM, but field implementation of these two techniques remains a problem. Therefore, LM is recommended as the gold standard for diagnosis of gestational malaria and placental blood infection in the field.

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2011-06-01
2017-11-19
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References

  1. López-Antuñano F, López-Antuñano FJ, Schmunis G, , 1988. Diagnóstico microscópico de los parásitos de la malaria en la sangre. , eds. Diagnóstico de malaria. Washington DC, WHO-PAHO, 3950.
  2. Makler M, Palmer C, Ager A, , 1998. A review of practical techniques for the diagnosis of malaria. Ann Trop Med Parasitol 92: 419433.[Crossref]
  3. PAHO, 1996. Pan American Health Organization. Malaria in the Americas. PAHO Bull 17: 118.
  4. Blair S, Carmona-Fonseca J, Piñeros J, Ríos A, Álvarez T, Álvarez G, Tobón A, , 2006. Therapeutic efficacy test in malaria falciparum in Antioquia, Colombia. Malar J 5: 14.[Crossref]
  5. Carmona-Fonseca J, Álvarez G, Blair S, , 2006. Plasmodium vivax malaria: treatment of primany attacks with primaquine, in three different doses, and a fixed dose of chloroquine, Antioquia, Colombia, 2003–2004. Biomedica 26: 353365.[Crossref]
  6. Castañeda A, , 1990. Niveles de Parasitemia por Plasmodium falciparum y Algunos Factores Relacionados. Urabá Bajo Cauca, Departamento de Antioquia; 1988–1989. Tesis de Grado. Medellín (Colombia): Universidad de Anioquia, Medellín (Colombia).
  7. Graffeo R, Masucci L, Bugli F, Damiano F, Pinnetti C, Federico G, Fadda G, , 2008. Multiple malaria infection in a pregnant woman from Nigeria: detection by multiplex PCR. New Microbiol 31: 565567.
  8. WHO, 2009. World Health Organization. Malaria Rapid Diagnostic Test Performance: Results of WHO Product Testing of Malaria RDTs: Round 1 (2008). Geneva: WHO.
  9. Pava Z, Echeverry D, Díaz G, Murillo C, , 2010. Large variation in detection of histidine-rich protein 2 in Plasmodium falciparum isolates from Colombia. Am J Trop Med Hyg 83: 834837.[Crossref]
  10. Gamboa D, Ho M, Bendezu J, Torres K, Chiodini P, Barnwell J, Incardona S, Perkins M, Bell D, McCarthy J, Cheng Q, , 2010. A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests. PLoS ONE 5: e8091.[Crossref]
  11. Snounou G, Viriyakosol S, Zhu X, Jarra W, Pinheiro L, Do Rosario V, Thaithong S, Brown K, , 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315320.[Crossref]
  12. Perandin F, Manca N, Calderaro A, Piccolo G, Galati L, Ricci L, Medici M, Arcangeletti M, Snounou G, Dettori G, Chezzi C, , 2004. Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J Clin Microbiol 42: 12141219.[Crossref]
  13. Lucchi N, Demas A, Narayanan J, Sumari D, Kabanywanyi A, Kachur S, Barnwell J, Udhayakumar V, , 2010. Real-time fluorescence loop mediated isothermal amplification for the diagnosis of malaria. PLoS One 5: e13733.[Crossref]
  14. Han E, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T, , 2007. Detection of four Plasmodium species by genus- and species-specific Loop-Mediated Isothermal Amplification for clinical diagnosis. J Clin Microbiol 45: 25212528.[Crossref]
  15. Paris D, Imwong M, Faiz A, Hasan M, Yunus E, Silamut K, Lee S, Day N, Dondorp A, , 2007. Loop-mediated isothermal PCR (LAMP) for the diagnosis of falciparum malaria. Am J Trop Med Hyg 77: 972976.
  16. Poon L, Wong B, Ma E, Chan Q, Chow L, Abeyewickreme W, Tangpukdee N, Yuen K, Guan Y, Looareesuwan S, Peiris J, , 2006. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem 52: 303306.[Crossref]
  17. Snounou G, , 2007. Rapid, sensitive and cheap molecular diagnosis of malaria: is microscopy on the way out? Future Microbiol 2: 477480.[Crossref]
  18. Rogerson S, Mkundika P, Kanjala M, , 2003. Diagnosis of Plasmodium falciparum malaria at delivery: comparison of blood film preparation methods and of blood films with histology. J Clin Microbiol 41: 13701374.[Crossref]
  19. Bulmer J, Rasheed F, Francis N, Morrison L, Greenwoods B, , 1993. Placental malaria. I. Pathological classification. Histopathology 22: 211218.[Crossref]
  20. Bulmer J, Rasheed F, Morrison L, Francis N, Greenwood B, , 1993. Placental malaria. II. A semi-quantitative investigation of the pathological features. Histopathology 22: 219225.[Crossref]
  21. Rogerson S, Pollina E, Getachew A, Tadesse E, Lema V, Molyneux M, , 2003. Placental monocyte infiltrates in response to Plasmodium falciparum malaria infection and their association with adverse pregnancy outcomes. Am J Trop Med Hyg 68: 115119.
  22. Uneke C, , 2008. Diagnosis of Plasmodium falciparum malaria in pregnancy in sub-Saharan Africa: the challenges and public health implications. Parasitol Res 102: 333342.[Crossref]
  23. Grobusch M, Hänscheid T, Göbels K, Slevogt H, Zoller T, Rögler G, Teichmann D, , 2003. Comparison of three antigen detection tests for diagnosis and follow-up of falciparum malaria in travellers returning to Berlin, Germany. Parasitol Res 89: 354357.
  24. Iqbal J, Khalid N, Hira P, , 2002. Comparison of two commercial assays with expert microscopy for confirmation of symptomatically diagnosed malaria. J Clin Microbiol 40: 46754678.[Crossref]
  25. Londoño B, Carmona J, Blair S, , 2002. Comparación de los métodos Optimal y gota gruesa para el diagnóstico de malaria en una zona endémica sin epidemia. Biomedica 22: 466475.[Crossref]
  26. Montoya A, Menco J, Osorio N, Zuluaga M, Duque J, Torres G, Restrepo M, , 2008. Concordance between thick blood smear, immunochromatography and polymerase chain reaction for malaria diagnosis. Biomedica 28: 252261.[Crossref]
  27. Pabón A, Alvarez G, Yánez J, Céspedes C, Rodríguez Y, Restrepo A, Blair S, , 2007. Evaluation of ICT malaria immunochromatograpic Binax NOW ICT Pf/Pv test for rapid diagnosis of malaria in a Columbian endemic area. Biomedica 27: 225235.[Crossref]
  28. Playford E, Walker J, , 2002. Evaluation of the ICT malaria Pf/Pv and the OptiMAL rapid diagnostic tests for malaria in febrile returned travellers. J Clin Microbiol 40: 41664171.[Crossref]
  29. Hanscheid T, , 1999. Diagnosis of malaria: a review of alternatives to conventional microscopy. Clin Lab Haematol 21: 235245.[Crossref]
  30. Jaimes F, , 2007. Pruebas diagnósticas: uso e interpretación. Acta Med Colomb 32: 2933.
  31. Pita Fernández S, Pértegas Díaz S, , 2003. Diagnostic tests. Cad Aten Primaria 10: 120124. Available at: http://www.fisterra.com/mbe/investiga/pruebas_diagnosticas/pruebas_diagnosticas122.pdf. Accessed December 13, 2009.
  32. Hernández J, , 2003. Cost-effectiveness of laboratory testing. Arch Pathol Lab Med 127: 440445.
  33. Carmona-Fonseca J, , 2003. La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca: panorama para interpretar la falla terapéutica antimalárica. Parte 1. Iatreia 16: 299318.
  34. Carmona-Fonseca J, , 2004. La malaria en Colombia, Antioquia y las zonas de Urabá y Bajo Cauca: panorama para interpretar la falla terapéutica antimalárica. Parte 2. Iatreia. 17: 3453.
  35. Carmona-Fonseca J, Maestre A, , 2009. Incidencia de la malaria gestacional, congénita y placentaria en Urabá (Antioquia, Colombia), 2005–2007. Rev Colomb Obstet Ginecol 60: 1226.
  36. García C, Moreno L, García H, Moreno L, Cano F, García H, , 1994. Evaluación de pruebas diagnósticas. , eds. Epidemiología clínica. Second edition. México DF: Editorial Interamericana S.A; 159162.
  37. Martínez-Bencardino C, , 1984. Muestreo. Bogotá: Ecoe, 4547.
  38. Landeta J, , 1999. The Delphi Method: A Technique for the Uncertainty Estimate. Barcelona: Ariel.
  39. Lagerberg R, , 2008. Malaria in pregnancy: a literature review. J Midwifery Womens Health 53: 209215.[Crossref]
  40. Adegnika A, Verweij J, Agnandji S, Chai S, Breitling L, Ramharter M, Frolich M, Issifou S, Kremsner P, Yazdanbakhsh M, , 2006. Microscopic and sub-microscopic Plasmodium falciparum infection, but not inflammation caused by infection, is associated with low birth weight. Am J Trop Med Hyg 75: 798803.
  41. Walker-Abbey A, Djokam R, Eno A, Leke R, Titanji V, Fogako J, Sama G, Thuita L, Beardslee E, Snounou G, Zhou A, Taylor D, , 2005. Malaria in pregnant Cameroonian women: the effect of age and gravidity on submicroscopic and mixed-species infections and multiple parasite genotypes. Am J Trop Med Hyg 72: 229235.
  42. Färnert A, , 2008. Plasmodium falciparum population dynamics: only snapshots in time? Trends Parasitol 24: 340344.[Crossref]
  43. Färnert A, Lebbad M, Faraja L, Rooth I, , 2008. Extensive dynamics of Plasmodium falciparum densities, stages and genotyping profiles. Malar J 2: 241.[Crossref]
  44. Beck S, Mockenhaupt F, Bienzle U, Eggelte T, Thompson W, Stark K, , 2001. Multiplicity of Plasmodium falciparum infection in pregnancy. Am J Trop Med Hyg 65: 631636.
  45. Arango E, Maestre A, Carmona-Fonseca J, , 2010. Effect of submicroscopic or polyclonal Plasmodium falciparum infection on mother and gestation product: systematic review. Rev bras epidemiol 13: 373386.[Crossref]
  46. Ndao M, , 2009. Diagnosis of parasitic diseases: old and new approaches. Interdiscip Perspect Infect Dis 2009: 278246.
  47. Abraira V, , 2006. Bias in studies of diagnostic tests. Semergen 32: 2426.[Crossref]
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  • Received : 09 Sep 2010
  • Accepted : 11 Feb 2011

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