Volume 84, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Malaria and filariasis are transmitted in the Southwest Pacific region by sibling species including , , the complex 1–8 (includes [ 2], [ 3]). Distinguishing these species from each other requires molecular diagnostic methods. We developed a multiplex polymerase chain reaction (PCR)–based assay specific for known species-specific nucleotide differences in the internal transcribed spacer 2 region and identified the five species most frequently implicated in transmitting disease (, , 1, , and 4). A set of 340 individual mosquitoes obtained from seven Papua New Guinea provinces representing a variety of habitats were analyzed by using this multiplex assay. Concordance between molecular and morphological diagnosis was 56.4% for , 85.3% for , and 88.9% for . Among 158 mosquitoes morphologically designated as , 33 were re-classified by PCR as , 4 as , 26 as 1, 49 as , and 46 as 4. Misclassification results from variable coloration of the proboscis and overlap of , , the 4. This multiplex technology enables further mosquito strain identification and simultaneous detection of microbial pathogens.


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  • Received : 04 Aug 2010
  • Accepted : 04 Oct 2010
  • Published online : 05 Jan 2011

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