Volume 84, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A recurrent focus of infestation was investigated in a suburban area of southern California after reports of suspected Rocky Mountain spotted fever in two dogs on the same property. Abundant quantities of were collected on the property and repeatedly from each dog, and DNA was detected by polymerase chain reaction (PCR). Whole blood and serum samples from four dogs were tested by using PCR and microimmunofluorescent assay for antibodies against spotted fever group rickettsiae. Serum samples from all four dogs contained antibodies reactive with , , , and 364D but no rickettsial DNA was detected by PCR of blood samples. Serum cross-absorption and Western blot assays implicated as the most likely spotted fever group rickettsiae responsible for seropositivity. To our knowledge, this is the first detection of in ticks in California.


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  1. Beati L, Finidori JP, Gilot B, Raoult D, , 1992. Comparison of serologic typing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis, and genetic restriction fragment length polymorphism analysis for identification of rickettsiae: characterization of two new rickettsial strains. J Clin Microbiol 30: 19221930. [Google Scholar]
  2. Beati L, Raoult D, , 1993. Rickettsia massiliae sp. nov., a new spotted fever group Rickettsia . Int J Syst Bacteriol 43: 839840.[Crossref] [Google Scholar]
  3. Babalis T, Tselentis Y, Roux V, Psaroulaki A, Raoult D, , 1994. Isolation and identification of a rickettsial strain related to Rickettsia massiliae in Greek ticks. Am J Trop Med Hyg 50: 365372. [Google Scholar]
  4. Beati L, Roux V, Ortuno A, Castella J, Porta FS, Raoult D, , 1996. Phenotypic and genotypic characterization of spotted fever group Rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks. J Clin Microbiol 34: 26882694. [Google Scholar]
  5. Eremeeva M, Bosserman E, Demma L, Zambrano M, Blau D, Dasch G, , 2006. Isolation and identification of Rickettsia massiliae from Rhipicephalus sanguineus ticks collected in Arizona. Appl Environ Microbiol 72: 55695577.[Crossref] [Google Scholar]
  6. Dupont HT, Cornet JP, Raoult D, , 1994. Identification of rickettsiae from ticks collected in the Central African Republic using the polymerase chain reaction. Am J Trop Med Hyg 50: 373380. [Google Scholar]
  7. Bernasconi M, Casati S, Peter O, Piffaretti J-C, , 2002. Rhipicephalus ticks infected with Rickettsia and Coxiella in southern Switzerland (Canton Ticino). Inf Gen Evol 2: 111120.[Crossref] [Google Scholar]
  8. Bacellar F, Regnery RL, Nuncio MS, Filipe AR, , 1995. Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal. Epidemiol Infect 114: 169178.[Crossref] [Google Scholar]
  9. Bitam I, Parola P, Matsumoto K, Rolain JM, Baziz B, Boubidi SC, Harrat Z, Belkaid M, Raoult D, , 2006. First molecular detection of R. conorii, R. aeschlimannii, and R. massiliae in ticks from Algeria. Ann N Y Acad Sci 1078: 368372.[Crossref] [Google Scholar]
  10. Fernandez-Soto P, Perez-Sanchez R, Diaz Martin V, Encinas-Grandes A, Alamo Sanz R, , 2006. Rickettsia massiliae in ticks removed from humans in Castilla y León, Spain. Eur J Clin Microbiol Infect Dis 25: 811813.[Crossref] [Google Scholar]
  11. Marquez F, , 2008. Spotted fever group Rickettsia in ticks from southeastern Spain natural parks. Exp Appl Acarol 45: 185194.[Crossref] [Google Scholar]
  12. Marquez FJ, Rodriguez-Libana JJ, Soriguer RC, Munian MA, Bernabeu-Wittel M, Caruz A, Contreras-Chova F, , 2008. Spotted fever group Rickettsia in brown dog ticks Rhipicephalus sanguineus in southwestern Spain. Parasitol Res 103: 119122.[Crossref] [Google Scholar]
  13. Psaroulaki A, Ragiadakou D, Kouris G, Papadopoulos B, Chaniotis B, Tselentis Y, , 2006. Ticks, tick-borne rickettsiae, and Coxiella burnetii in the Greek Island of Cephalonia. Ann N Y Acad Sci 1078: 389399.[Crossref] [Google Scholar]
  14. Santos-Silva M, Sousa R, Santos A, Melo P, Encarnao V, Bacellar F, , 2006. Ticks parasitizing wild birds in Portugal: detection of Rickettsia aeschlimannii, R. helvetica and R. massiliae . Exp Appl Acarol 39: 331338.[Crossref] [Google Scholar]
  15. Sarih M, Socolovschi C, Boudebouch N, Hassar M, Raoult D, Parola P, , 2008. Spotted fever group rickettsiae in ticks, Morocco. Emerg Infect Dis 14: 10671073.[Crossref] [Google Scholar]
  16. Berrelha J, Briolant S, Muller F, Rolain JM, Marie JL, Pagés F, Raoult D, Parola P, , 2009. Rickettsia felis and Rickettsia massiliae in Ivory Coast, Africa. Clin Microbiol Infect 15: 251252.[Crossref] [Google Scholar]
  17. Harrus S, Perlman-Avrahami A, Mumcuoglu KY, Morick D, Baneth G, , 2010. Molecular detection of Rickettsia massiliae, Rickettsia sibirica mongolitimonae and Rickettsia conorii israelensis in ticks from Israel. Clin Microbiol Infect Mar 20 [Epub ahead of print]. [Google Scholar]
  18. Cicuttin GL, Rodríguez-Vargas M, Jado I, Anda P, , 2004. Primera detección de Rickettsia massiliae en la ciudad de Buenos Aires. Resultados preliminaries. Rev Argentina Zoon 1: 810. [Google Scholar]
  19. Vitale G, Mansuelo S, Rolain J-M, Raoult D, , 2006. Rickettsia massiliae human isolation. Emerg Infect Dis 12: 174175.[Crossref] [Google Scholar]
  20. Parola P, Socolovschi C, Jeanjean L, Bitam I, Fournier P-E, Sotto A, Labauge P, Raoult D, , 2008. Warmer weather linked to tick attack and emergence of severe rickettsioses. PLoS Negl Trop Dis 2: e338e338.[Crossref] [Google Scholar]
  21. Garcia-Garcia J, Portillo A, Nez M, Santibez S, Castro B, Oteo J, , 2010. A patient from Argentina infected with Rickettsia massiliae . Am J Trop Med Hyg 82: 691692.[Crossref] [Google Scholar]
  22. Bella F, Espejo E, Uriz S, Serrano JA, Alegre MD, Tort J, , 1991. Randomized trial of 5-day rifampin versus 1-day doxycycline therapy for Mediterranean spotted fever. J Infect Dis 164: 433434.[Crossref] [Google Scholar]
  23. Rolain JM, Maurin M, Vestris G, Raoult D, , 1998. In vitro susceptibilities of 27 rickettsiae to 13 antimicrobials. Antimicrob Agents Chemother 42: 15371541. [Google Scholar]
  24. Pons I, Nogueras MM, Sanfeliu I, Ortuno A, Segura F, , 2008. Serological evidence of infection with Bar29 in dog population of Catalonia, in the northeast of Spain. 5th International Meeting on Rickettsiae and Rickettsial Diseases. Abstract Book, Marseille, France. [Google Scholar]
  25. Ortuno A, Pons I, Nogueras MM, Castella J, Segura F, , 2009. The dog as an epidemiological marker of Rickettsia conorii infection. Clin Microbiol Infect 15: 241242.[Crossref] [Google Scholar]
  26. Paddock C, Brenner O, Vaid C, Boyd DB, Berg J, Joseph R, Zaki S, Childs J, , 2002. Short report: concurrent Rocky Mountain spotted fever in a dog and its owner. Am J Trop Med Hyg 66: 197199. [Google Scholar]
  27. Elchos B, Goddard J, , 2003. Implications of presumptive Rocky Mountain spotted fever in two dogs and their owner. J Am Vet Med Assoc 223: 14501452, 1433.[Crossref] [Google Scholar]
  28. Paddock C, Koss T, Eremeeva M, Dasch G, Zaki S, Sumner J, , 2006. Isolation of Rickettsia akari from eschars of patients with rickettsialpox. Am J Trop Med Hyg 75: 732738. [Google Scholar]
  29. Laemmli UK, , 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680685.[Crossref] [Google Scholar]
  30. Eremeeva ME, Balayeva NM, Ignatovich VF, Raoult D, , 1993. Proteinic and genomic identification of spotted fever group rickettsiae isolated in the former USSR. J Clin Microbiol 31: 26252633. [Google Scholar]
  31. Jensenius M, Fournier P-E, Vene S, Ringertz S, Myrvang B, Raoult D, , 2004. Comparison of immunofluorescence, Western blotting, and cross-adsorption assays for diagnosis of African tick bite fever. Clin Diagn Lab Immunol 11: 786788. [Google Scholar]
  32. Beati L, Keirans JE, , 2001. Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. J Parasitol 87: 3248.[Crossref] [Google Scholar]
  33. Wikswo M, Hu R, Dasch G, Krueger L, Arugay A, Jones K, Hess B, Bennett S, Kramer V, Eremeeva M, , 2008. Detection and identification of spotted fever group rickettsiae in Dermacentor species from southern California. J Med Entomol 45: 509516.[Crossref] [Google Scholar]
  34. Solano-Gallego L, Kidd L, Trotta M, Di Marco M, Caldin M, Furlanello T, Breitschwerdt E, , 2006. Febrile illness associated with Rickettsia conorii infection in dogs from Sicily. Emerg Infect Dis 12: 19851988.[Crossref] [Google Scholar]
  35. Kelly PJ, Mattheaman LA, Mason PR, Courtney S, Katsande C, Rukwava J, , 1992. Experimental infection of dogs with a Zimbabwean strain of Rickettsia conorii . J Trop Med Hyg 95: 322326. [Google Scholar]
  36. Norment BR, Burgdorfer W, , 1984. Susceptibility and reservoir potential of the dog to spotted fever group rickettsiae. Am J Vet Res 45: 17061710. [Google Scholar]
  37. Breitschwerdt EB, Walker DH, Levy MG, Burgdorfer W, Corbett WT, Hurlbert SA, Stebbins ME, Curtis BC, Allen DA, , 1988. Clinical, hematologic, and humoral immune response in female dogs inoculated with Rickettsia rickettsii and Rickettsia montana . Am J Vet Res 49: 7076. [Google Scholar]
  38. Greene CE, Breitschwerdt EB, Green CE, , 2006. Rocky Mountain spotted fever, murine typhuslike disease, rickettsialpox, typhus, and Q fever. , ed. Infectious Diseases of the Dog and Cat. St. Louis: Saunders-Elsevier, 232245. [Google Scholar]
  39. Labruna M, Kamakura O, Moraes-Filho J, Horta M, Pacheco R, , 2009. Rocky Mountain spotted fever in dogs, Brazil. Emerg Infect Dis 15: 458460.[Crossref] [Google Scholar]
  40. Eremeeva ME, Moriarity J, Bosserman EA, Zambrano ML, Nicholson WL, Paddock CD, Demma L, Traeger M, Johnson B, Dickson J, Levin ML, Swerdlow D, McQuiston JH, Dasch GA, , 2006. Detection of rickettsiae in Rhipicephalus sanguineus ticks from Arizona. 20th Meeting of the American Society for Rickettsiology and the 5th International Conference on Bartonella as Emerging Pathogens. Asilomar, CA. [Google Scholar]
  41. Matsumoto K, Ogawa M, Brouqui P, Raoult D, Parola P, , 2005. Transmission of Rickettsia massiliae in the tick, Rhipicephalus turanicus . Med Vet Ent 19: 263270.[Crossref] [Google Scholar]
  42. Wikswo M, Hu R, Metzger M, Eremeeva M, , 2007. Detection of Rickettsia rickettsii and Bartonella henselae in Rhipicephalus sanguineus ticks from California. J Med Entomol 44: 158162.[Crossref] [Google Scholar]
  43. Carpenter TL, McMeans MC, McHugh CP, , 1990. Additional instances of human parasitism by the brown dog tick (Acari: Ixodidae). J Med Entomol 27: 10651066.[Crossref] [Google Scholar]
  44. Felz MW, Durden LA, Oliver JH, , 1996. Ticks parasitizing humans in Georgia and South Carolina. J Parasitol 82: 505508.[Crossref] [Google Scholar]
  45. Goddard J, , 1989. Focus of human parasitism by the brown dog tick, Rhipicephalus sanguineus (Acari: Ixodidae). J Med Entomol 26: 628629.[Crossref] [Google Scholar]
  46. Demma L, Traeger M, Nicholson W, Paddock C, Blau D, Eremeeva M, Dasch G, Levin M, Singleton J, Zaki S, Cheek J, Swerdlow D, McQuiston J, , 2005. Rocky Mountain spotted fever from an unexpected tick vector in Arizona. N Engl J Med 353: 587594.[Crossref] [Google Scholar]
  47. Fox MT, Sykes TJ, , 1985. Establishment of the tropical dog tick, Rhipicephalus sanguineus, in a house in London. Vet Rec 116: 661662.[Crossref] [Google Scholar]
  48. Uspensky I, Ioffe-Uspensky I, , 2002. The dog factor in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) infestations in and near human dwellings. Int J Med Microbiol 291 (Suppl 33): 156163.[Crossref] [Google Scholar]
  49. Brouqui P, Raoult D, , 2006. Arthropod-borne diseases in homeless. Ann N Y Acad Sci 1078: 223235.[Crossref] [Google Scholar]

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  • Received : 22 Jun 2010
  • Accepted : 01 Nov 2010
  • Published online : 04 Feb 2011

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