1921
Volume 84, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

A seroepidemiological investigation was conducted among the population of two municipalities in Northeastern Brazil. Immunoglobulin M (IgM) and IgG antibodies to were positive in 51.27% (161 in 317 samples) and 58.49% (186), respectively. IgM titers were higher in children than in adults. On the contrary, IgG increased progressively with age. We observed a significant association between agricultural occupation and raised IgM titers ( < 0.005) and IgG titers ( < 0.001), and between construction workers and raised IgG titers ( = 0.005). Antibody IgG avidities did not correlate with age. The highest titers of antibodies (1/800) showed the highest antibody avidity indexes ( < 0.01). Most of the serum samples recognized 45-kDa and 200-kDa bands by IgG1 and IgG2 subclasses. Our study showed a high seropositivity among individuals living in endemic regions of the state of Ceará, and highlights the need for further surveillance close to water courses such as dams and rivers in Northeastern Brazil.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2011.10-0220
2011-02-04
2017-11-22
Loading full text...

Full text loading...

/deliver/fulltext/14761645/84/2/302.html?itemId=/content/journals/10.4269/ajtmh.2011.10-0220&mimeType=html&fmt=ahah

References

  1. Cheng AC, Currie BJ, , 2005. Melioidosis: epidemiology, pathophysiology and management. Clin Microbiol Rev 18: 383416.[Crossref]
  2. Rolim DB, Vilar DC, Sousa AQ, Miralles IS, Oliveira DC, Harnett G, O'Reilly L, Howard K, Sampson I, Inglis TJ, , 2005. Melioidosis, northeastern Brazil. Emerg Infect Dis 11: 14581460.[Crossref]
  3. Anuntagool N, Intachote P, Wuthiekanun V, White NJ, Sirisinha S, , 1998. Lipopolysaccharide from nonvirulent Ara+ Burkholderia pseudomallei isolates is immunologically indistinguishable from lipopolysaccharide from virulent Ara-clinical isolates. Clin Diagn Lab Immunol 5: 225229.
  4. Ashdown L, , 1981. Relationship and significance of specific immunoglobulin M antibody response in clinical and subclinical melioidosis. J Clin Microbiol 14: 361364.
  5. Chentamarakshan V, Vadivelu J, Puthucheary SD, , 2001. Detection of immunoglobulins M and G using culture filtrate antigen of Burkholderia pseudomallei . Diagn Microbiol Infect Dis 39: 17.[Crossref]
  6. Inglis TJ, Rolim DB, Sousa AQ, , 2006. Melioidosis in the Americas. Am J Trop Med Hyg 75: 947954.
  7. Inglis TJ, Merritt A, Chidlow G, Aravena-Roman M, Harnett G, , 2005. Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei . J Clin Microbiol 43: 22012206.[Crossref]
  8. Alexander AD, Huxsoll DH, Warner AR, Shepler V, Dorsey A, , 1970. Serological diagnosis of human melioidosis with indirect hemagglutination and complement fixation tests. Appl Microbiol 20: 825833.
  9. Nagao AT, Carneiro-Sampaio MM, Carlsson B, Hanson LA, , 1995. Antibody titer and avidity in saliva and serum are not impaired in mildly to moderately undernourished children. J Trop Pediatr 41: 153157.[Crossref]
  10. Wuthiekanun V, Peaktra N, Putchhat H, Sin L, Sen B, Kumar V, Langla S, Peacock SJ, Day NP, , 2008. Burkholderia pseudomallei antibodies in children, Cambodia. Emerg Infect Dis 14: 301303.[Crossref]
  11. Rolim DB, Rocha MF, Brilhante RS, Cordeiro RA, Leitão-Junior NP, Inglis TJ, , 2009. Environmental isolates of Burkholderia pseudomallei in Ceará State, northeast Brazil. Appl Environ Microbiol 75: 12151218.[Crossref]
  12. Chenthamarakshan V, Kumutha MV, Vadivelu J, Puthucheary SD, , 2001. Distribution of immunoglobulin classes and IgG subclasses against a culture filtrate antigen of Burkholderia pseudomallei in melioidosis patients. J Med Microbiol 50: 5561.[Crossref]
  13. Chantatrita N, Wuthiekanun V, Thanwisai A, Limmathurotsakul D, Cheng AC, Chierakul W, , 2007. Accuracy of ELISA using crude and purified antigens for the serodiagnosis of melioidosis. Clin Vaccine Immunol 14: 110113.[Crossref]
  14. Anuntagool N, Sirisinha S, , 2002. Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei . Microbiol Immunol 46: 143150.[Crossref]
  15. Nimtz M, Wray V, Domke T, Brenneke B, Häussler S, Steinmetz I, , 1997. Structure of an acidic exopolysaccharide of Burkholderia pseudomallei . Eur J Biochem 250: 608616.[Crossref]
  16. Anuntagool N, Wuthiekanun V, White NJ, Currie BJ, Sermswan RW, Wongratanacheewin S, , 2006. Lipopolysaccharide heterogeneity among Burkholderia pseudomallei from different geographic and clinical origins. Am J Trop Med Hyg 74: 348352.
  17. Pontes GN, Massironi SG, Arslanian C, Palmeira P, Carneiro-Sampaio MM, Nagao AT, , 2005. Human IgG but not IgM antibodies can protect mice from the challenge with live O6 Escherichia coli . Scand J Immunol 62: 353360.[Crossref]
  18. Usinger WR, Lucas AH, , 1999. Avidity as a determinant of the protective efficacy of human antibodies to pneumococcal capsular polysaccharides. Infect Immun 67: 23662370.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2011.10-0220
Loading
/content/journals/10.4269/ajtmh.2011.10-0220
Loading

Data & Media loading...

  • Received : 15 Apr 2010
  • Accepted : 01 Nov 2010

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error