Volume 85, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



cultivation of parasites plays an important role in diagnosis and treatment of leishmaniasis and in vaccine and drug development studies. Conversely, long-term cultivation of parasites usually results in decreased infectivity potential. Some studies reported a stimulatory effect of human urine in promastigotes. However, there is no information about the effects of urine within culture on the infectivity of parasites. Analysis of the effect of urine have showed that proliferation indexes were significantly increased in culture medium supplemented with human urine ( = 38.17 ± 5.12, = 34.74 ± 5.6, = 34.22 ± 4.66, and 35.88 ± 6.40) than in controls. Infection indexes were 13 ± 1.7 for , 55 ± 2.2 for , 41 ± 3.14 for , and 49 ± 3.26 for . Our results showed that human urine increased the infectivity and proliferation of parasites.


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  • Received : 09 Apr 2010
  • Accepted : 24 Jun 2011

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