1921
Volume 84, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The objective of this study was to evaluate the behavior of different tests used for the diagnosis of visceral leishmaniasis (VL) in asymptomatic subjects living in an endemic area. No gold standard is available for the diagnosis of asymptomatic infection with . In continuation of a previous study, 1,017 subjects living in a VL-endemic area were clinically reevaluated. Of these, 576 had at least one positive serological test in a first assessment. About 3 years after the first evaluation, none of the subjects had progressed to clinical VL. Among this group, 246 subjects were selected, and five serological tests (enzyme-linked immunosorbent assay p [ELISAp], ELISArK39, ELISArK26, indirect immunofluorescence test [IIFT] using promastigote antigen, and an immunochromatographic test using rK39 antigen [TRALd]) and the Montenegro skin test (MST) were repeated. There was a significant increase in the number of subjects who tested positive in the MST, IIFT, ELISAp, and ELISArK39 in the second evaluation. For all tests, there were subjects who tested positive in the first evaluation and negative in the second evaluation. A positive result in the serological tests and MST in subjects from the endemic area studied did not indicate a risk of progression to VL and may only be temporary.

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2011-02-04
2017-09-23
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References

  1. Brasil Ministério da Saúde, Fundação Secretaria de Vigilância em Saúde, Departamento de Vigilância Epidemiológica, 2006. Manual de vigilância e controle da leishmaniose visceral. Brasil: Ministério da Saúde. 1122.
  2. Deane MP, Deane LM, , 1955. Observações sobre a transmissão da leishmaniose visceral no Ceará. Hospital 48: 347364.
  3. Costa CHN, Gomes RBB, Silva MRB, Garcez LM, Ramos PKS, Santos RS, Shaw JJ, David JR, Maguire JH, , 2000. Competence of human host as a reservoir for Leishmania chagasi . J Infect Dis 182: 9971000.[Crossref]
  4. Moreno EC, Gonçalves AV, Chaves AV, Melo MN, Lambertucci JR, Andrade ASR, Corrêa DN, Antunes CMF, Carneiro M, , 2009. Inaccuracy of enzyme-linked immunosorbent assay using soluble and recombinant antigens to detect asymptomatic infection by Leishmania infantum . PLoS Negl Trop Dis 5: e536.[Crossref]
  5. Manson-Bahr PEC, Southgate BA, , 1964. Recent research on Kala Azar in East Africa. J Trop Med Hyg 67: 7984.
  6. Shido SA, Akuffo HO, Mohamed AA, Huldt G, Nilsson LA, Ouchterlony O, Thorstensson R, , 1995. Visceral leishmaniasis in Somalia: prevalence of leishmanin-positive and seropositive inhabitants in an endemic area. Trans R Soc Trop Med Hyg 89: 2124.[Crossref]
  7. Cabello PH, Lima AMVM, Azevedo ES, Krieger H, , 1995. Familial aggregation of Leishmania chagasi infection in northeastern Brazil. Am J Trop Med Hyg 53: 364365.
  8. D'Oliveira A, Jr Costa SRM, Barbosa AB, Orge MGO, Carvalho EM, , 1997. Asymptomatic Leishmania chagasi infection in relatives and neighbors of patients with visceral leishmaniasis. Mem Inst Oswaldo Cruz 92: 1520.[Crossref]
  9. Badaró R, Jones TC, Carvalho EM, Sampaio DP, Reed SG, Barral A, Teixeira R, Johnson WD, Jr, 1986. New perspectives on a sub clinical form of visceral leishmaniasis. J Infect Dis 154: 10031011.[Crossref]
  10. Evans TG, Teixeira MJ, Mcauliffe IT, Vasconcelos IAB, Vasconcelos AW, Sousa AQ, Lima JWO, Pearson RD, , 1992. Epidemiology of visceral leishmaniasis in northeast Brazil. J Infect Dis 166: 11241132.[Crossref]
  11. Jerônimo SMB, Teixeira MJ, Sousa AQ, Thielking P, Pearson RD, Evans TG, , 2000. Natural history of Leishmania (Leishmania) chagasi infection in northeastern Brazil: long-term follow-up. Clin Infect Dis 30: 608609.[Crossref]
  12. Burns JM, Jr Shreffler WG, Benson DR, Ghalib HW, Badaró R, Reed SG, , 1993. Molecular characterization of a kinesin-related antigen of Leishmania chagasi that detects specific antibody in African and American visceral leishmaniasis. Proc Natl Acad Sci USA 90: 775779.[Crossref]
  13. Romero HD, Silva LA, Silva-Vergara ML, Rodrigues V, Costa RT, Carvalho SFG, Alecrim W, Moraes-Souza H, Prata A, , 2009. Comparative study of serologic tests for the diagnosis of asymptomatic visceral leishmaniasis in an endemic area. Am J Trop Med Hyg 81: 2733.
  14. Borges VC, Ruiz MCM, Gomes PM, Colombo AR, Silva LA, Romero HD, Prata A, , 2003. Intradermorreação de Montenegro após sucessivas repetições do teste em Porteirinha, MG. Rev Soc Bras Med Trop 36: 249251.[Crossref]
  15. Camargo ME, , 1966. Fluorescent antibody test for the serodiagnosis of American trypanosomiasis. Technical modification employing preserved culture forms of Trypanosoma cruzi in a slide test. Rev Inst Med Trop Sao Paulo 8: 227234.
  16. Melo MN, Mayrink W, Costa CA, Magalhães PA, Dias M, Williams P, Araújo FG, Coelho MV, Batista SM, , 1977. Padronização do antígeno de Montenegro. Rev Inst Med Trop Sao Paulo 19: 161164.
  17. Sokal JE, , 1975. Editorial: measurement of delayed skin-test response. N Engl J Med 293: 501502.[Crossref]
  18. Badaró R, Reed SG, Barral A, Orge MGO, Jones TC, , 1986. Evaluation of micro enzyme-linked immunosorbent assay (ELISA) for antibodies in American visceral leishmaniasis: antigen selection for detection of infection-specific response. Am J Trop Med Hyg 35: 7278.
  19. Hommel M, Peters W, Ranque J, Quilici M, Lanotte G, , 1978. The micro-ELISA technique in the serodiagnosis of visceral leishmaniasis. Ann Trop Med Parasitol 72: 213218.[Crossref]
  20. Voller A, Bidwell DE, Bartlett A, , 1976. Enzyme immunoassay in diagnostic medicine. Theory and practice. Bull World Health Organ 53: 5565.
  21. Brazil Ministry of Health, 1996. National Research Council. Resolution 196/96. Brazil: Ministry of Health.
  22. Singh S, Kumari V, Singh N, , 2002. Predicting kala-azar disease manifestation in asymptomatic patients with latent Leishmania donovani infection by detection of antibody against recombinant k39 antigen. Clin Diagn Lab Immunol 9: 568572.
  23. Nascimento MDSB, Neto APBN, Silva NL, Coaracy GAV, Souza EC, Silva MH, Lindoso E, Sousa WB, Magalhães CA, Brito GO, Almeida PEA, Bezerra GFB, Viana GMC, , 2000. Progressão da intradermorreação de Montenegro e a sororreatividade por ELISA com os antígenos rk39 e crude em indivíduos assintomáticos de área endêmica de leishmaniose visceral. Rev Soc Bras Med Trop 33 (Suppl 1): 41.
  24. Viana GMC, Nascimento MDSB, Viana MGC, Burattini MN, , 2000. Avaliação prospectiva do antígeno rK39 como indicador de doença ativa em leishmaniose visceral. Rev Soc Bras Med Trop 33 (Suppl 1): 319320.
  25. Moreno EC, Melo MN, Lambertucci JR, Serufo JC, Andrade ASR, Antunes CMF, Genaro O, Carneiro M, , 2006. Diagnosing human asymptomatic visceral leishmaniasis in an urban area of the State of Minas Gerais, using serological and molecular biology techniques. Rev Soc Bras Med Trop 39: 421427.[Crossref]
  26. De Luca PM, Mayrink W, Santiago MA, Nogueira R, Conceição-Silva F, Melo G, Mendonça SC, , 2003. Randomized, double-blind, placebo-controlled study on the immunogenicity of the leishmanin skin test. Trans R Soc Trop Med Hyg 97: 709712.[Crossref]
  27. Bern C, Amann J, Haque R, Chowdhury AM, Kurkjian KM, Vaz L, Wagatsuma Y, Breiman RF, Secro WE, Maguire JH, , 2006. Loss of leishmanin skin test antigen sensitivity and potency in a longitudinal study of visceral leishmaniasis in Bangladesh. Am J Trop Med Hyg 75: 744748.
  28. Southgate BA, Oriedo VEB, , 1967. Studies in the epidemiology of East African Leishmaniasis. 3. Immunity as a determinant of geographical distribution. J Trop Med Hyg 70: 14.
  29. Southgate BA, Manson-Bahr PEC, , 1967. Studies in the epidemiology of East African Leishmaniasis. 4. The significance of the positive leishmanin test. J Trop Med Hyg 70: 2933.
  30. Levy L, Mendes E, , 1981. Impaired cell-mediated immunity in patients with Kala-azar. Allergol Immunopathol (Madr) 9: 109112.
  31. Pampiglione S, Manson-Bahr PE, La Placa M, Borgatti MA, Musumeci S, , 1975. Studies in Mediterranean leishmaniasis. 3. The leishmanin skin test in kala-azar. Trans R Soc Trop Med Hyg 69: 6068.[Crossref]
  32. Silva LA, Romero HD, Prata A, Costa RT, Nascimento E, Carvalho SFG, Rodrigues V, , 2006. Immunologic tests in patients after clinical cure of visceral leishmaniasis. Am J Trop Med Hyg 75: 739743.
  33. Neogy AB, Nandy A, Dastidar BG, Chowdhury AB, , 1986. Leishmanin skin test in Indian kala-azar. Trans R Soc Trop Med Hyg 80: 454455.[Crossref]
  34. Gidwani K, Rai M, Chakravarty J, Boelaert M, Sundar S, , 2009. Short report: evaluation of leishmanin skin test in Indian visceral leishmaniasis. Am J Trop Med Hyg 80: 566567.
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  • Received : 10 Feb 2010
  • Accepted : 26 Oct 2010

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