Volume 83, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Physical mapping is a useful approach for studying genome organization and evolution as well as for genome sequence assembly. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to develop high-resolution physical maps. We report a 0.6-Mb-resolution physical map consisting of 422 DNA markers hybridized to 379 chromosomal sites of the polytene chromosomes. This makes second only to in density of a physical map among malaria mosquitoes. Three hundred sixty-three (363) probes hybridized to single chromosomal sites, whereas 59 clones yielded multiple signals. This physical map provided a suitable basis for comparative genomics, which was used for determining inversion breakpoints, duplications, and origin of novel genes across species.


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Supplementary figure and tables

  • Received : 28 Jun 2010
  • Accepted : 30 Jul 2010
  • Published online : 05 Nov 2010

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