1921
Volume 83, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Physical mapping is a useful approach for studying genome organization and evolution as well as for genome sequence assembly. The availability of polytene chromosomes in malaria mosquitoes provides a unique opportunity to develop high-resolution physical maps. We report a 0.6-Mb-resolution physical map consisting of 422 DNA markers hybridized to 379 chromosomal sites of the polytene chromosomes. This makes second only to in density of a physical map among malaria mosquitoes. Three hundred sixty-three (363) probes hybridized to single chromosomal sites, whereas 59 clones yielded multiple signals. This physical map provided a suitable basis for comparative genomics, which was used for determining inversion breakpoints, duplications, and origin of novel genes across species.

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References

  1. Zhimulev IF, , 1996. Morphology and Structure of Polytene Chromosomes. San Diego, CA: Academic Press, 1490. [Google Scholar]
  2. Zheng L, Saunders RD, Fortini D, della Torre A, Coluzzi M, Glover DM, Kafatos FC, , 1991. Low-resolution genome map of the malaria mosquito Anopheles gambiae . Proc Natl Acad Sci USA 88: 1118711191.[Crossref] [Google Scholar]
  3. Zheng L, Benedict MQ, Cornel AJ, Collins FH, Kafatos FC, , 1996. An integrated genetic map of the African human malaria vector mosquito, Anopheles gambiae . Genetics 143: 941952. [Google Scholar]
  4. Dimopoulos G, Zheng L, Kumar V, della Torre A, Kafatos FC, Louis C, , 1996. Integrated genetic map of Anopheles gambiae: use of RAPD polymorphisms for genetic, cytogenetic and STS landmarks. Genetics 143: 953960. [Google Scholar]
  5. della Torre A, Favia G, Mariotti G, Coluzzi M, Mathiopoulos KD, , 1996. Physical map of the malaria vector Anopheles gambiae . Genetics 143: 13071311. [Google Scholar]
  6. Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, Nusskern DR, Wincker P, Clark AG, Ribeiro JM, Wides R, Salzberg SL, Loftus B, Yandell M, Majoros WH, Rusch DB, Lai Z, Kraft CL, Abril JF, Anthouard V, Arensburger P, Atkinson PW, Baden H, de Berardinis V, Baldwin D, Benes V, Biedler J, Blass C, Bolanos R, Boscus D, Barnstead M, Cai S, Center A, Chaturverdi K, Christophides GK, Chrystal MA, Clamp M, Cravchik A, Curwen V, Dana A, Delcher A, Dew I, Evans CA, Flanigan M, Grundschober-Freimoser A, Friedli L, Gu Z, Guan P, Guigo R, Hillenmeyer ME, Hladun SL, Hogan JR, Hong YS, Hoover J, Jaillon O, Ke Z, Kodira C, Kokoza E, Koutsos A, Letunic I, Levitsky A, Liang Y, Lin JJ, Lobo NF, Lopez JR, Malek JA, McIntosh TC, Meister S, Miller J, Mobarry C, Mongin E, Murphy SD, O'Brochta DA, Pfannkoch C, Qi R, Regier MA, Remington K, Shao H, Sharakhova MV, Sitter CD, Shetty J, Smith TJ, Strong R, Sun J, Thomasova D, Ton LQ, Topalis P, Tu Z, Unger MF, Walenz B, Wang A, Wang J, Wang M, Wang X, Woodford KJ, Wortman JR, Wu M, Yao A, Zdobnov EM, Zhang H, Zhao Q, Zhao S, Zhu SC, Zhimulev I, Coluzzi M, della Torre A, Roth CW, Louis C, Kalush F, Mural RJ, Myers EW, Adams MD, Bork P, Brey PT, Venter JC, Weissenbach J, Kafatos FC, Collins FH, Hoffman SL, , 2002. The genome sequence of the malaria mosquito Anopheles gambiae . Science 298: 129149.[Crossref] [Google Scholar]
  7. Sharakhova M, Hammond MP, Lobo NF, Krzywinski J, Unger MF, Hillenmeyer ME, Bruggner RV, Birney E, Collins FH, , 2007. Update of the Anopheles gambiae PEST genome assembly. Genome Biol 8: R5.[Crossref] [Google Scholar]
  8. Sharakhova MV, Xia A, McAlister SI, Sharakhov IV, , 2006. A standard cytogenetic photomap for the mosquito Anopheles stephensi (Diptera: Culicidae): application for physical mapping. J Med Entomol 43: 861866.[Crossref] [Google Scholar]
  9. Xia A, Sharakhova M, Leman S, Tu Z, Bailey J, Smith C, Sharakhov IV, , 2010. Genome landscape and evolutionary plasticity of chromosomes in malaria mosquitoes. PLoS ONE 5: e10592.[Crossref] [Google Scholar]
  10. Xia A, Sharakhova MV, Sharakhov IV, , 2008. Reconstructing ancestral autosomal arrangements in the Anopheles gambiae complex. J Comput Biol 15: 965980.[Crossref] [Google Scholar]
  11. Lawson D, Arensburger P, Atkinson P, Besansky NJ, Bruggner RV, Butler R, Campbell KS, Christophides GK, Christley S, Dialynas E, Hammond M, Hill CA, Konopinski N, Lobo NF, MacCallum RM, Madey G, Megy K, Meyer J, Redmond S, Severson DW, Stinson EO, Topalis P, Birney E, Gelbart WM, Kafatos FC, Louis C, Collins FH, , 2009. VectorBase: a data resource for invertebrate vector genomics. Nucleic Acids Res 37: D583D587.[Crossref] [Google Scholar]
  12. Rai KS, Black WC, 4th, 1999. Mosquito genomes: structure, organization, and evolution. Adv Genet 41: 133. [Google Scholar]
  13. Patil DP, Atanur S, Dhotre DP, Anantharam D, Mahajan VS, Walujkar SA, Chandode RK, Kulkarni GJ, Ghate PS, Srivastav A, Dayananda KM, Gupta N, Bhagwat B, Joshi RR, Mourya DT, Patole MS, Shouche YS, , 2009. Generation, annotation, and analysis of ESTs from midgut tissue of adult female Anopheles stephensi mosquitoes. BMC Genomics 10: 386.[Crossref] [Google Scholar]
  14. Sharakhov IV, Serazin AC, Grushko OG, Dana A, Lobo N, Hillenmeyer ME, Westerman R, Romero-Severson J, Costantini C, Sagnon N, Collins FH, Besansky NJ, , 2002. Inversions and gene order shuffling in Anopheles gambiae and A. funestus . Science 298: 182185.[Crossref] [Google Scholar]
  15. Gonzalez J, Ranz JM, Ruiz A, , 2002. Chromosomal elements evolve at different rates in the Drosophila genome. Genetics 161: 11371154. [Google Scholar]
  16. Xing J, Wang H, Belancio VP, Cordaux R, Deininger PL, Batzer MA, , 2006. Emergence of primate genes by retrotransposon-mediated sequence transduction. Proc Natl Acad Sci U S A 103: 1760817613.[Crossref] [Google Scholar]
  17. Long M, Betran E, Thornton K, Wang W, , 2003. The origin of new genes: glimpses from the young and old. Nat Rev Genet 4: 865875.[Crossref] [Google Scholar]
  18. Gonzalez J, Casals F, Ruiz A, , 2004. Duplicative and conservative transpositions of larval serum protein 1 genes in the genus Drosophila . Genetics 168: 253264.[Crossref] [Google Scholar]
  19. Serazin AC, Dana AN, Hillenmeyer ME, Lobo NF, Coulibaly MB, Willard MB, Harker BW, Sharakhov IV, Collins FH, Ribeiro JM, Besansky NJ, , 2009. Comparative analysis of the global transcriptome of Anopheles funestus from Mali, West Africa. PLoS ONE 4: e7976.[Crossref] [Google Scholar]
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Supplementary figure and tables

  • Received : 28 Jun 2010
  • Accepted : 30 Jul 2010

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