Volume 83, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Standardized antimycobacterial therapy is considered the treatment of choice for Buruli ulcer disease. To assess the prevalence of drug resistance among clinical isolates in Ghana, we conducted a sequence-based approach to detect mutations associated with drug resistance. We subjected clinical samples to direct DNA sequencing of and genes and compared culture and whole-genome extracts regarding the efficiency of sequence analysis; 99.1% () and 100% () of the patients harbored wild type. In one isolate (0.9%), a point mutation of the gene at codon Ser522 leading to an amino acid change was detected. Culture extracts yielded a significantly higher sequencing efficiency than whole-genome extracts. Our data suggest a low level of drug resistance in Ghana. However, mutations associated with drug resistance do occur and require monitoring. Improved techniques are necessary to enhance the efficiency of sequence analysis of whole-genome extracts.


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  • Received : 09 May 2010
  • Accepted : 30 Jul 2010
  • Published online : 05 Nov 2010

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