Volume 83, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



mosquitoes are the primary vector responsible for transmission in Macha, Zambia. Because insecticide-treated bed nets (ITNs) have the potential to alter host feeding behavior, the extent of the zoophilic and exophagic tendencies of the vector was evaluated during the two rainy seasons after ITN introduction. Centers for Disease Control light traps, paired indoor/outdoor human landing catches, and outdoor cattle-baited collections were used to assess potential changes in host preference. Results support the hypothesis that mosquitoes in Macha remain highly anthropophilic despite high ITN use. mosquitoes in Macha appear to be relatively exophagic and have been caught biting outdoors immediately after sunset and before sunrise, potentially circumventing some of the protective effects of ITNs.


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  1. Ministry of Health of Zambia, 2006. A Six-Year Strategic Plan: A Roadmap for the Impact on Malaria in Zambia, 2006–2011. Available at: http://www.nmcc.org.zm/files/6NMCPStrategicPlanZMOH.doc. [Google Scholar]
  2. Chanda E, Masaninga F, Coleman M, Sikaala C, Katebe C, Macdonald M, Baboo KS, Govere J, Manga L, , 2008. Integrated vector management: the Zambian experience. Malar J 7: 164.[Crossref] [Google Scholar]
  3. Kent RJ, Thuma PE, Mharakurwa S, Norris DE, , 2007. Seasonality, blood feeding behavior, and transmission of Plasmodium falciparum by Anopheles arabiensis after an extended drought in southern Zambia. Am J Trop Med Hyg 76: 267274. [Google Scholar]
  4. Gillies MT, DeMeillon B, , 1968. The Anophelinae of Africa South of the Sahara (Ethiopian Zoogeographical Region). Second edition. Johannesburg: South African Institute for Medical Research. [Google Scholar]
  5. White GB, Magayuka SA, Brocham PF, , 1972. Comparative studies on sibling species of the Anopheles gambiae Giles complex (Diptera: Culicidae): bionomics and vectorial activity at Segera, Tanzania. Bull Entomol Res 62: 295317.[Crossref] [Google Scholar]
  6. Antonio-Nkondjio C, Kerah CH, Simard F, Awono-Ambene P, Chouaibou M, Tchuinkam T, Fontenille D, , 2006. Complexity of the malaria vectorial system in Cameroon: contribution of secondary vectors to malaria transmission. J Med Entomol 43: 12151221.[Crossref] [Google Scholar]
  7. Taye A, Hadis M, Adugna N, Tilahun D, Wirtz RA, , 2006. Biting behavior and Plasmodium infection rates of anopheles arabiensis from Sille, Ethiopia. Acta Trop 97: 5054.[Crossref] [Google Scholar]
  8. Kerah-Hinzoumbe C, Peka M, Antonio-Nkondjio C, Donan-Gouni I, Awono-Ambene P, Same-Ekobo A, Simard F, , 2009. Malaria vectors and transmission dynamics in Goulmoun, a rural city in south-western Chad. BMC Infect Dis 9: 71.[Crossref] [Google Scholar]
  9. Fontenille D, Lochouarn L, Diatta M, Sokhna C, Dia I, Diagne N, Lemasson JJ, Ba K, Tall A, Rogier C, Trape JF, , 1997. Four years' entomological study of the transmission of seasonal malaria in Senegal and the bionomics of Anopheles gambiae and A. arabiensis . Trans R Soc Trop Med Hyg 91: 647652.[Crossref] [Google Scholar]
  10. Lemasson JJ, Fontenille D, Lochouarn L, Dia I, Simard F, Ba K, Diop A, Diatta M, Molez J, , 1997. Comparison of behavior and vector efficiency of Anopheles gambiae and An. arabiensis (Diptera: Culicidae) in Barkedji, a Sahelian area of Senegal. J Med Entomol 34: 396403.[Crossref] [Google Scholar]
  11. Ameneshewa B, Service MW, , 1996. Resting habits of Anopheles arabiensis in the Awash River Valley of Ethiopia. Ann Trop Med Parasitol 90: 515521.[Crossref] [Google Scholar]
  12. Tirados I, Costantini C, Gibson G, Torr SJ, , 2006. Blood-feeding behaviour of the malarial mosquito Anopheles arabiensis: implications for vector control. Med Vet Entomol 20: 425437.[Crossref] [Google Scholar]
  13. Mendis C, Jacobsen JL, Gamage-Mendis A, Bule E, Dgedge M, Thompson R, Cuamba N, Barreto J, Begtrup K, Sinden RE, Hogh B, , 2000. Anopheles arabiensis and An. funestus are equally important vectors of malaria in Matola coastal suburb of Maputo, southern Mozambique. Med Vet Entomol 14: 171180.[Crossref] [Google Scholar]
  14. Geissbuhler Y, Chaki P, Emidi B, Govella NJ, Shirima R, Mayagaya V, Mtasiwa D, Mshinda H, Fillinger U, Lindsay SW, Kannady K, de Castro MC, Tanner M, Killeen GF, , 2007. Interdependence of domestic malaria prevention measures and mosquito-human interactions in urban Dar es Salaam, Tanzania. Malar J 6: 126.[Crossref] [Google Scholar]
  15. Oyewole I, Awolola T, Ibidapo C, Oduola A, Okwa O, Obansa J, , 2007. Behaviour and population dynamics of the major anopheline vectors in a malaria endemic area in southern Nigeria. J Vector Borne Dis 44: 56. [Google Scholar]
  16. Ralisoa Randrianasolo BO, Coluzzi M, , 1987. Genetical investigations on zoophilic and exophilic Anopheles arabiensis from Antananarivo area (Madagascar). Parassitologia 29: 9397. [Google Scholar]
  17. Mahande A, Mosha F, Mahande J, Kweka E, , 2007. Feeding and resting behaviour of malaria vector, Anopheles arabiensis with reference to zooprophylaxis. Malar J 6: 100.[Crossref] [Google Scholar]
  18. Adugna N, Petros B, , 1996. Determination of the human blood index of some anopheline mosquitos by using ELISA. Ethiop Med J 34: 110. [Google Scholar]
  19. Hadis M, Lulu M, Makonnen Y, Asfaw T, , 1997. Host choice by indoor-resting Anopheles arabiensis in Ethiopia. Trans R Soc Trop Med Hyg 91: 376378.[Crossref] [Google Scholar]
  20. Garrett-Jones C, Boreham PF, Pant CP, , 1980. Feeding habits of anophelines (Diptera: Culicidae) in 1971–78, with reference to the human blood index: a review. Bull Entomol Res 70: 165185.[Crossref] [Google Scholar]
  21. Joshi GP, Service MW, Pradhan GD, , 1975. A survey of species A and B of the Anopheles gambiae Giles complex in the Kisumu area of Kenya prior to insecticidal spraying with OMS-43 (fenitrothion). Ann Trop Med Parasitol 69: 91104.[Crossref] [Google Scholar]
  22. Fornadel CM, Norris DE, , 2008. Increased endophily by the malaria vector Anopheles arabiensis in southern Zambia and identification of digested blood meals. Am J Trop Med Hyg 79: 876880. [Google Scholar]
  23. Muriu SM, Muturi EJ, Shililu JI, Mbogo CM, Mwangangi JM, Jacob BG, Irungu LW, Mukabana RW, Githure JI, Novak RJ, , 2008. Host choice and multiple blood feeding behaviour of malaria vectors and other anophelines in Mwea rice scheme, Kenya. Malar J 7: 43.[Crossref] [Google Scholar]
  24. Githeko AK, Adungo NI, Karanja DM, Hawley WA, Vulule JM, Seroney IK, Ofulla AV, Atieli FK, Ondijo SO, Genga IO, Odada PK, Situbi PA, Oloo JA, , 1996. Some observations on the biting behavior of Anopheles gambiae s.s., Anopheles arabiensis, and Anopheles funestus and their implications for malaria control. Exp Parasitol 82: 306315.[Crossref] [Google Scholar]
  25. Bøgh C, Clarke SE, Pinder M, Sanyang F, Lindsay SW, , 2001. Effect of passive zooprophylaxis on malaria transmission in The Gambia. J Med Entomol 38: 822828.[Crossref] [Google Scholar]
  26. Magesa SM, Wilkes TJ, Mnzava AE, Njunwa KJ, Myamba J, Kivuyo MD, Hill N, Lines JD, Curtis CF, , 1991. Trial of pyrethroid impregnated bednets in an area of Tanzania holoendemic for malaria. Part 2. Effects on the malaria vector population. Acta Trop 49: 97108.[Crossref] [Google Scholar]
  27. Gimnig JE, Kolczak MS, Hightower AW, Vulule JM, Schoute E, Kamau L, Phillips-Howard PA, ter Kuile FO, Nahlen BL, Hawley WA, , 2003. Effect of permethrin-treated bed nets on the spatial distribution of malaria vectors in western Kenya. Am J Trop Med Hyg 68: 115. [Google Scholar]
  28. Sampath TR, Yadav RS, Sharma VP, Adak T, , 1998. Evaluation of lambdacyhalothrin-impregnated bednets in a malaria endemic area of India. Part 2. Impact on malaria vectors. J Am Mosq Control Assoc 14: 437443. [Google Scholar]
  29. Mbogo CN, Baya NM, Ofulla AV, Githure JI, Snow RW, , 1996. The impact of permethrin-impregnated bednets on malaria vectors of the Kenyan coast. Med Vet Entomol 10: 251259.[Crossref] [Google Scholar]
  30. Charlwood JD, Graves PM, , 1987. The effect of permethrin-impregnated bednets on a population of Anopheles farauti in coastal Papua New Guinea. Med Vet Entomol 1: 319327.[Crossref] [Google Scholar]
  31. Bøgh C, Pedersen EM, Mukoko DA, Ouma JH, , 1998. Permethrin-impregnated bednet effects on resting and feeding behaviour of lymphatic filariasis vector mosquitoes in Kenya. Med Vet Entomol 12: 5259.[Crossref] [Google Scholar]
  32. Lines JD, Myamba J, Curtis CF, , 1987. Experimental hut trials of permethrin-impregnated mosquito nets and eave curtains against malaria vectors in Tanzania. Med Vet Entomol 1: 3751.[Crossref] [Google Scholar]
  33. Miller JE, Lindsay SW, Armstrong JR, , 1991. Experimental hut trials of bednets impregnated with synthetic pyrethroid or organophosphate insecticide for mosquito control in The Gambia. Med Vet Entomol 5: 465476.[Crossref] [Google Scholar]
  34. Gillies MT, Coetzee M, , 1987. A Supplement to the Anophelinae of Africa South of the Sahara. Johannesburg: South African Institute for Medical Research. [Google Scholar]
  35. Kent RJ, Norris DE, , 2005. Identification of mammalian blood meals in mosquitoes by a multiplexed polymerase chain reaction targeting cytochrome B. Am J Trop Med Hyg 73: 336342. [Google Scholar]
  36. Scott JA, Brogdon WG, Collins FH, , 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49: 520529. [Google Scholar]
  37. Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, , 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315320.[Crossref] [Google Scholar]
  38. Mathenge EM, Misiani GO, Oulo DO, Irungu LW, Ndegwa PN, Smith TA, Killeen GF, Knols BG, , 2005. Comparative performance of the Mbita trap, CDC light trap and the human landing catch in the sampling of Anopheles arabiensis, An. funestus and culicine species in a rice irrigation in western Kenya. Malar J 4: 7.[Crossref] [Google Scholar]
  39. Mathenge EM, Omweri GO, Irungu LW, Ndegwa PN, Walczak E, Smith TA, Killeen GF, Knols BG, , 2004. Comparative field evaluation of the Mbita trap, the Centers for Disease Control light trap, and the human landing catch for sampling of malaria vectors in western Kenya. Am J Trop Med Hyg 70: 3337. [Google Scholar]
  40. Hii JL, Smith T, Mai A, Ibam E, Alpers MP, , 2000. Comparison between anopheline mosquitoes (Diptera: Culicidae) caught using different methods in a malaria endemic area of Papua New Guinea. Bull Entomol Res 90: 211219.[Crossref] [Google Scholar]
  41. Lindsay SW, Alonso PL, Schellenberg JR, Hemingway J, Adiamah JH, Shenton FC, Jawara M, Greenwood BM, , 1993. A malaria control trial using insecticide-treated bed nets and targeted chemoprophylaxis in a rural area of The Gambia, West Africa: 7. Impact of permethrin-impregnated bed nets on malaria vectors. Trans R Soc Trop Med Hyg 87: 4551.[Crossref] [Google Scholar]
  42. Yohannes M, Haile M, Ghebreyesus TA, Witten KH, Getachew A, Byass P, Lindsay SW, , 2005. Can source reduction of mosquito larval habitat reduce malaria transmission in Tigray, Ethiopia? Trop Med Int Health 10: 12741285.[Crossref] [Google Scholar]
  43. Fornadel CM, Norris LC, Norris DE, , 2010. Centers for Disease Control light traps for monitoring Anopheles arabiensis human biting rates in an area with low vector density and high insecticide-treated bed net use. Am J Trop Med Hyg 83: 838842.[Crossref] [Google Scholar]
  44. Githeko AK, Service MW, Mbogo CM, Atieli FA, Juma FO, , 1994. Sampling Anopheles arabiensis, A. gambiae sensu lato and A. funestus (Diptera: Culicidae) with CDC light-traps near a rice irrigation area and a sugarcane belt in western Kenya. Bull Entomol Res 84: 319324.[Crossref] [Google Scholar]
  45. Davis JR, Hall T, Chee EM, Majala A, Minjas J, Shiff CJ, , 1995. Comparison of sampling anopheline mosquitoes by light-trap and human-bait collections indoors at Bagamoyo, Tanzania. Med Vet Entomol 9: 249255.[Crossref] [Google Scholar]
  46. Govella NJ, Chaki PP, Geissbuhler Y, Kannady K, Okumu F, Charlwood JD, Anderson RA, Killeen GF, , 2009. A new tent trap for sampling exophagic and endophagic members of the Anopheles gambiae complex. Malar J 8: 157.[Crossref] [Google Scholar]
  47. Mbogo CN, Glass GE, Forster D, Kabiru EW, Githure JI, Ouma JH, Beier JC, , 1993. Evaluation of light traps for sampling anopheline mosquitoes in Kilifi, Kenya. J Am Mosq Control Assoc 9: 260263. [Google Scholar]

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  • Received : 27 Apr 2010
  • Accepted : 15 Jun 2010
  • Published online : 05 Oct 2010

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