Volume 83, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Species-specific diagnosis was performed in 66 patients with cutaneous leishmaniasis (CL) living in Tataouine focus in southeastern Tunisia. DNA was extracted directly from dermal scrapings (n = 66) and from parasites obtained in culture (n = 12). Species were identified by using polymerase chain reaction–restriction fragment length polymorphism analysis for internal transcribed spacer region 1 and isoenzyme analysis. and were identified in 31 (47%) and 35 (53%) cases respectively. CL cases were geographically scattered, and CL cases were clustered. Lesions caused by were mostly single (83.8%) and face-localized (55.8%), and lesions caused by were multiple (57.1%; < 0.001) and situated on limbs (83.7%; < 0.001). For both species, most lesion onsets were reported during June–January. However, lesions that emerged during February–May were mainly caused by (83.3%; < 0.01). Moreover, the delay before seeking medical advice was higher for infections than for infections ( < 0.05).


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  1. Rioux JA, Lanotte G, Pratlong F, Rioux JA, , 1986. Leishmania killicki n.sp. (Kinetoplastida, Trypanosomatidae). , ed. Leishmania: Taxonomie et Phylogenèse; Applications Éco-Épidémiologiques. Montpellier, France: IMEEE, 139142. [Google Scholar]
  2. Pratlong F, Dereure J, Ravel C, Balard Y, Serres G, Lanotte G, Rioux JA, , 2009. Geographical distribution and epidemiological features of old world cutaneous leishmaniasis foci, based on the isoenzyme analysis of 1,048 strains. Trop Med Int Health 14: 10711085.[Crossref] [Google Scholar]
  3. Aoun K, Amri F, Chouihi E, Haouas N, Bedoui K, Benikhlef R, Ghrab J, Babba H, Chahed MK, Harrat Z, Bouratbine A, , 2008. Epidémiologie de Leishmania (L.) infantum, L. major et L. killicki en Tunisie: résultats et analyse de l'identification de 226 isolats humains et canins et revue de la littérature. Bull Soc Pathol Exot 101: 323328.[Crossref] [Google Scholar]
  4. Haouas N, Chergui N, Chaker E, Ben Said M, Babba H, Belhadj S, Kallel K, Pratlong F, Dedet JP, Mezhoud H, Azaiez R, , 2005. Anthroponotic cutaneous leishmaniasis in Tunisia: presence of Leishmania killicki outside its original focus of Tataouine. Trans R Soc Trop Med Hyg 99: 499501.[Crossref] [Google Scholar]
  5. Maubon D, Thurot-Guillou C, Ravel C, Leccia MT, Pelloux H, , 2009. Leishmania killicki imported from the Tunisian desert. Emerg Infect Dis 15: 18641865.[Crossref] [Google Scholar]
  6. Vega-Lopez F, , 2003. Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 16: 97101.[Crossref] [Google Scholar]
  7. Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, Jaffe CL, , 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47: 349358.[Crossref] [Google Scholar]
  8. Garcia AL, Parrado R, De Doncker S, Bermudez H, Dujardin JC, , 2007. American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples. Trans R Soc Trop Med Hyg 101: 368371.[Crossref] [Google Scholar]
  9. Chouihi E, Amri F, Bouslimi N, Siala E, Selmi K, Zallagua N, Ben Abdallah R, Bouratbine A, Aoun K, , 2009. Les cultures sur milieu NNN dans le diagnostic biologique des leishmanioses. Pathol Biol (Paris) 57: 219224.[Crossref] [Google Scholar]
  10. Rioux JA, Lanotte G, Serres E, Pratlong F, Bastien P, Perieres J, , 1990. Taxonomy of Leishmania. Use of isoenzymes: suggestion for a new classification. Ann Parasitol Hum Comp 65: 111115.[Crossref] [Google Scholar]
  11. Desjeux P, , 2001. The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg 95: 239243.[Crossref] [Google Scholar]
  12. Ben Ismail R, Ben Rachid MS, , 1989. Epidémiologie des leishmanioses en Tunisie. Maladies Tropicales Transmissibles. Paris: AUPELF-UREF, 7380. [Google Scholar]
  13. Ghrab J, Rhim A, Bach Hamba D, Chahed MK, Aoun K, Nouira S, Bouratbine A, , 2006. Phlebotominae (Diptera: Psychodidae) of human leishmaniosis sites in Tunisia. Parasite 13: 2333.[Crossref] [Google Scholar]
  14. Faulde M, Schrader J, Heyl G, Amirith M, , 2008. Differences in transmission seasons as an epidemiological tool for characterization of anthroponotic and zoonotic cutaneous leishmaniasis in northern Afghanistan. Acta Trop 105: 131138.[Crossref] [Google Scholar]
  15. Chaffai M, Ben Rachid MS, Ben Ismail R, Ben Osman A, Makni K, , 1998. Formes clinico-épidémiologiques de la leishmaniose cutanée en Tunisie. Ann Dermatol Venereol 115: 12551260. [Google Scholar]
  16. Bryceson AD, Rioux JA, , 1986. Clinical variations associated with various taxa of Leishmania . , ed. Leishmania: Taxonomie et Phylogenèse; Applications Éco-Épidémiologiques. Montpellier, France: IMEEE, 221228. [Google Scholar]

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  • Received : 23 Apr 2010
  • Accepted : 22 Jul 2010
  • Published online : 05 Nov 2010

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