1921
Volume 83, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Species-specific diagnosis was performed in 66 patients with cutaneous leishmaniasis (CL) living in Tataouine focus in southeastern Tunisia. DNA was extracted directly from dermal scrapings (n = 66) and from parasites obtained in culture (n = 12). Species were identified by using polymerase chain reaction–restriction fragment length polymorphism analysis for internal transcribed spacer region 1 and isoenzyme analysis. and were identified in 31 (47%) and 35 (53%) cases respectively. CL cases were geographically scattered, and CL cases were clustered. Lesions caused by were mostly single (83.8%) and face-localized (55.8%), and lesions caused by were multiple (57.1%; < 0.001) and situated on limbs (83.7%; < 0.001). For both species, most lesion onsets were reported during June–January. However, lesions that emerged during February–May were mainly caused by (83.3%; < 0.01). Moreover, the delay before seeking medical advice was higher for infections than for infections ( < 0.05).

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  • Received : 23 Apr 2010
  • Accepted : 22 Jul 2010

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