1921
Volume 83, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Species-specific diagnosis was performed in 66 patients with cutaneous leishmaniasis (CL) living in Tataouine focus in southeastern Tunisia. DNA was extracted directly from dermal scrapings (n = 66) and from parasites obtained in culture (n = 12). Species were identified by using polymerase chain reaction–restriction fragment length polymorphism analysis for internal transcribed spacer region 1 and isoenzyme analysis. and were identified in 31 (47%) and 35 (53%) cases respectively. CL cases were geographically scattered, and CL cases were clustered. Lesions caused by were mostly single (83.8%) and face-localized (55.8%), and lesions caused by were multiple (57.1%; < 0.001) and situated on limbs (83.7%; < 0.001). For both species, most lesion onsets were reported during June–January. However, lesions that emerged during February–May were mainly caused by (83.3%; < 0.01). Moreover, the delay before seeking medical advice was higher for infections than for infections ( < 0.05).

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References

  1. Rioux JA, Lanotte G, Pratlong F, 1986. Leishmania killicki n.sp. (Kinetoplastida, Trypanosomatidae). Rioux JA, ed. Leishmania: Taxonomie et Phylogenèse; Applications Éco-Épidémiologiques. Montpellier, France: IMEEE, 139142.
    [Google Scholar]
  2. Pratlong F, Dereure J, Ravel C, Balard Y, Serres G, Lanotte G, Rioux JA, 2009. Geographical distribution and epidemiological features of old world cutaneous leishmaniasis foci, based on the isoenzyme analysis of 1,048 strains. Trop Med Int Health 14: 10711085.[Crossref]
    [Google Scholar]
  3. Aoun K, Amri F, Chouihi E, Haouas N, Bedoui K, Benikhlef R, Ghrab J, Babba H, Chahed MK, Harrat Z, Bouratbine A, 2008. Epidémiologie de Leishmania (L.) infantum, L. major et L. killicki en Tunisie: résultats et analyse de l'identification de 226 isolats humains et canins et revue de la littérature. Bull Soc Pathol Exot 101: 323328.[Crossref]
    [Google Scholar]
  4. Haouas N, Chergui N, Chaker E, Ben Said M, Babba H, Belhadj S, Kallel K, Pratlong F, Dedet JP, Mezhoud H, Azaiez R, 2005. Anthroponotic cutaneous leishmaniasis in Tunisia: presence of Leishmania killicki outside its original focus of Tataouine. Trans R Soc Trop Med Hyg 99: 499501.[Crossref]
    [Google Scholar]
  5. Maubon D, Thurot-Guillou C, Ravel C, Leccia MT, Pelloux H, 2009. Leishmania killicki imported from the Tunisian desert. Emerg Infect Dis 15: 18641865.[Crossref]
    [Google Scholar]
  6. Vega-Lopez F, 2003. Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 16: 97101.[Crossref]
    [Google Scholar]
  7. Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, Jaffe CL, 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47: 349358.[Crossref]
    [Google Scholar]
  8. Garcia AL, Parrado R, De Doncker S, Bermudez H, Dujardin JC, 2007. American tegumentary leishmaniasis: direct species identification of Leishmania in non-invasive clinical samples. Trans R Soc Trop Med Hyg 101: 368371.[Crossref]
    [Google Scholar]
  9. Chouihi E, Amri F, Bouslimi N, Siala E, Selmi K, Zallagua N, Ben Abdallah R, Bouratbine A, Aoun K, 2009. Les cultures sur milieu NNN dans le diagnostic biologique des leishmanioses. Pathol Biol (Paris) 57: 219224.[Crossref]
    [Google Scholar]
  10. Rioux JA, Lanotte G, Serres E, Pratlong F, Bastien P, Perieres J, 1990. Taxonomy of Leishmania. Use of isoenzymes: suggestion for a new classification. Ann Parasitol Hum Comp 65: 111115.[Crossref]
    [Google Scholar]
  11. Desjeux P, 2001. The increase in risk factors for leishmaniasis worldwide. Trans R Soc Trop Med Hyg 95: 239243.[Crossref]
    [Google Scholar]
  12. Ben Ismail R, Ben Rachid MS, 1989. Epidémiologie des leishmanioses en Tunisie. Maladies Tropicales Transmissibles. Paris: AUPELF-UREF, 7380.
    [Google Scholar]
  13. Ghrab J, Rhim A, Bach Hamba D, Chahed MK, Aoun K, Nouira S, Bouratbine A, 2006. Phlebotominae (Diptera: Psychodidae) of human leishmaniosis sites in Tunisia. Parasite 13: 2333.[Crossref]
    [Google Scholar]
  14. Faulde M, Schrader J, Heyl G, Amirith M, 2008. Differences in transmission seasons as an epidemiological tool for characterization of anthroponotic and zoonotic cutaneous leishmaniasis in northern Afghanistan. Acta Trop 105: 131138.[Crossref]
    [Google Scholar]
  15. Chaffai M, Ben Rachid MS, Ben Ismail R, Ben Osman A, Makni K, 1998. Formes clinico-épidémiologiques de la leishmaniose cutanée en Tunisie. Ann Dermatol Venereol 115: 12551260.
    [Google Scholar]
  16. Bryceson AD, 1986. Clinical variations associated with various taxa of Leishmania . Rioux JA, ed. Leishmania: Taxonomie et Phylogenèse; Applications Éco-Épidémiologiques. Montpellier, France: IMEEE, 221228.
    [Google Scholar]
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  • Received : 23 Apr 2010
  • Accepted : 22 Jul 2010
  • Published online : 05 Nov 2010
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