1921
Volume 83, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Dengue virus serotype 2 (DENV-2) RNA replication profiles and tropisms were studied by using quantitative RT-PCR (q-RTPCR) in intrathoracically infected . The virus RNA replication profiles were diverse in mosquito organs. In fat body, brain, salivary gland, and malpighian tubes, it peaked at 8, 23, 23, and 27 days post-infection, respectively, and then, all declined. In midgut, it increased all the time and had no trend of decline. In ovary, it had no apparent increase. Subsequent Western blotting of DENV-2 E protein had similar results. Using ribosomal protein 7 (rpS7) as an internal control, we found that, in salivary gland, brain, fat body, and midgut, the average DENV-2 RNA levels (DENV-2 RNA/rpS7 mRNA) were 1,028, 464, 5.6, and 6.2, respectively; in malpighian tubes, it was 1, and in ovary, it was far less than 1. These results suggest that infection profiles and tropism of DENV-2 RNA in organs are significantly different.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2010.10-0193
2010-09-01
2017-05-29
Loading full text...

Full text loading...

/deliver/fulltext/14761645/83/3/700.html?itemId=/content/journals/10.4269/ajtmh.2010.10-0193&mimeType=html&fmt=ahah

References

  1. Gubler DJ, , 2002. The global emergence/resurgence of arboviral diseases as public health problems. Arch Med Res 33: 330342.[Crossref]
  2. Wilder-Smith A, Schwartz E, , 2005. Dengue in travelers. N Engl J Med 353: 924932.[Crossref]
  3. Guzman MG, Kouri G, , 2002. Dengue: an update. Lancet Infect Dis 2: 3342.[Crossref]
  4. Gratz NG, , 2004. Critical review of the vector status of Aedes albopictus . Med Vet Entomol 18: 215227.[Crossref]
  5. Woodring JL, Higgs S, Beaty BJ, Beaty BJ, Marquardt WC, , 1996. Natural cycles of vector-borne pathogens. , eds. The Biology of Disease Vectors. Niwot, CO: University Press of Colorado, 5172.
  6. Gubler DJ, Rosen L, , 1976. Variation among geographic strains of Aedes albopictus in susceptibility to infection with dengue viruses. Am J Trop Med Hyg 25: 318325.
  7. Gubler DJ, Nalim S, Tan R, Saipan H, Sulianti Saroso J, , 1979. Variation in susceptibility to oral infection with dengue viruses among geographic strains of Aedes aegypti . Am J Trop Med Hyg 28: 10451052.
  8. Tardieux I, Poupel O, Lapchin L, Rodhain F, , 1990. Variation among strains of Aedes aegypti in susceptibility to oral infection with dengue virus type 2. Am J Trop Med Hyg 43: 308313.
  9. Sumanochitrapon W, Strickman D, Sithiprasasna R, Kittayapong P, Innis BL, , 1998. Effect of size and geographic origin of Aedes aegypti on oral infection with dengue-2 virus. Am J Trop Med Hyg 58: 283286.
  10. Vazeille-Falcoz M, Mousson L, Rodhain F, Chungue E, Failloux AB, , 1999. Variation in oral susceptibility to dengue type 2 virus of populations of Aedes aegypti from the islands of Tahiti and Moorea, French Polynesia. Am J Trop Med Hyg 60: 292299.
  11. Bennett KE, Olson KE, Munoz Mde L, Fernandez-Salas I, Farfan-Ale JA, Higgs S, Black WCT, Beaty BJ, , 2002. Variation in vector competence for dengue 2 virus among 24 collections of Aedes aegypti from Mexico and the United States. Am J Trop Med Hyg 67: 8592.
  12. Vazeille M, Rosen L, Mousson L, Failloux AB, , 2003. Low oral receptivity for dengue type 2 viruses of Aedes albopictus from southeast Asia compared with that of Aedes aegypti . Am J Trop Med Hyg 68: 203208.
  13. Bosio CF, Beaty BJ, Black WCT, , 1998. Quantitative genetics of vector competence for dengue-2 virus in Aedes aegypti . Am J Trop Med Hyg 59: 965970.
  14. Bosio CF, Fulton RE, Salasek ML, Beaty BJ, Black WCT, , 2000. Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti . Genetics 156: 687698.
  15. Sylla M, Bosio C, Urdaneta-Marquez L, Ndiaye M, Black WCT, , 2009. Gene flow, subspecies composition, and dengue virus-2 susceptibility among Aedes aegypti collections in Senegal. PLoS Negl Trop Dis 3: e408.[Crossref]
  16. Sriurairatna S, Bhamarapravati N, , 1977. Replication of dengue-2 virus in Aedes albopictus mosquitoes. An electron microscopic study. Am J Trop Med Hyg 26: 11991205.
  17. Kuberski T, , 1979. Fluorescent antibody studies on the development of dengue-2 virus in Aedes albopictus (Diptera: Culicidae). J Med Entomol 16: 343349.[Crossref]
  18. Chen WJ, Wei HL, Hsu EL, Chen ER, , 1993. Vector competence of Aedes albopictus and Ae. aegypti (Diptera: Culicidae) to dengue 1 virus on Taiwan: development of the virus in orally and parenterally infected mosquitoes. J Med Entomol 30: 524530.[Crossref]
  19. Linthicum KJ, Platt K, Myint KS, Lerdthusnee K, Innis BL, Vaughn DW, , 1996. Dengue 3 virus distribution in the mosquito Aedes aegypti: an immunocytochemical study. Med Vet Entomol 10: 8792.[Crossref]
  20. Salazar MI, Richardson JH, Sanchez-Vargas I, Olson KE, Beaty BJ, , 2007. Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes. BMC Microbiol 7: 9.[Crossref]
  21. Molina-Cruz A, Gupta L, Richardson J, Bennett K, Black WT, Barillas-Mury C, , 2005. Effect of mosquito midgut trypsin activity on dengue-2 virus infection and dissemination in Aedes aegypti . Am J Trop Med Hyg 72: 631637.
  22. Richardson J, Molina-Cruz A, Salazar MI, Black WT, , 2006. Quantitative analysis of dengue-2 virus RNA during the extrinsic incubation period in individual Aedes aegypti . Am J Trop Med Hyg 74: 132141.
  23. Rosen L, Gubler D, , 1974. The use of mosquitoes to detect and propagate dengue viruses. Am J Trop Med Hyg 23: 11531160.
  24. Wu Y, Parthasarathy R, Bai H, Palli SR, , 2006. Mechanisms of midgut remodeling: juvenile hormone analog methoprene blocks midgut metamorphosis by modulating ecdysone action. Mech Dev 123: 530547.[Crossref]
  25. Gubler DJ, Novak RJ, Vergne E, Colon NA, Velez M, Fowler J, , 1985. Aedes (Gymnometopa) mediovittatus (Diptera: Culicidae), a potential maintenance vector of dengue viruses in Puerto Rico. J Med Entomol 22: 469475.[Crossref]
  26. Watts DM, Burke DS, Harrison BA, Whitmire RE, Nisalak A, , 1987. Effect of temperature on the vector efficiency of Aedes aegypti for dengue 2 virus. Am J Trop Med Hyg 36: 143152.
  27. Johnson BW, Chambers TV, Crabtree MB, Bhatt TR, Guirakhoo F, Monath TP, Miller BR, , 2002. Growth characteristics of ChimeriVax-DEN2 vaccine virus in Aedes aegypti and Aedes albopictus mosquitoes. Am J Trop Med Hyg 67: 260265.
  28. Armstrong PM, Rico-Hesse R, , 2001. Differential susceptibility of Aedes aegypti to infection by the American and southeast Asian genotypes of dengue type 2 virus. Vector Borne Zoonotic Dis 1: 159168.[Crossref]
  29. Armstrong PM, Rico-Hesse R, , 2003. Efficiency of dengue serotype 2 virus strains to infect and disseminate in Aedes aegypti . Am J Trop Med Hyg 68: 539544.
  30. Johnson BW, Chambers TV, Crabtree MB, Guirakhoo F, Monath TP, Miller BR, , 2004. Analysis of the replication kinetics of the ChimeriVax-DEN 1, 2, 3, 4 tetravalent virus mixture in Aedes aegypti by real-time reverse transcriptase-polymerase chain reaction. Am J Trop Med Hyg 70: 8997.
  31. Gubler DJ, Rosen L, , 1977. Quantitative aspects of replication of dengue viruses in Aedes albopictus (Diptera: Culicidae) after oral and parenteral infection. J Med Entomol 31: 469472.[Crossref]
  32. Romoser WS, Faran ME, Bailey CL, Lerdthusnee K, , 1992. An immunocytochemical study of the distribution of Rift Valley fever virus in the mosquito Culex pipiens . Am J Trop Med Hyg 46: 489501.
  33. Sanchez-Vargas I, Scott JC, Poole-Smith BK, Franz AW, Barbosa-Solomieu V, Wilusz J, Olson KE, Blair CD, , 2009. Dengue virus type 2 infections of Aedes aegypti are modulated by the mosquito's RNA interference pathway. PLoS Pathog 5: e1000299.[Crossref]
  34. Weaver SC, Scott TW, Lorenz LH, Lerdthusnee K, Romoser WS, , 1988. Togavirus-associated pathologic changes in the midgut of a natural mosquito vector. J Virol 62: 20832090.
  35. Romoser WS, Wasieloski LP, Jr Pushko P, Kondig JP, Lerdthusnee K, Neira M, Ludwig GV, , 2004. Evidence for arbovirus dissemination conduits from the mosquito (Diptera: Culicidae) midgut. J Med Entomol 41: 467475.[Crossref]
  36. Romoser WS, Turell MJ, Lerdthusnee K, Neira M, Dohm D, Ludwig G, Wasieloski L, , 2005. Pathogenesis of Rift Valley fever virus in mosquitoes—tracheal conduits and the basal lamina as an extra-cellular barrier. Arch Virol Suppl 19: 89100.
  37. Sanders HR, Foy BD, Evans AM, Ross LS, Beaty BJ, Olson KE, Gill SS, , 2005. Sindbis virus induces transport processes and alters expression of innate immunity pathway genes in the midgut of the disease vector, Aedes aegypti . Insect Biochem Mol Biol 35: 12931307.[Crossref]
  38. Bryant B, Blair CD, Olson KE, Clem RJ, , 2008. Annotation and expression profiling of apoptosis-related genes in the yellow fever mosquito, Aedes aegypti . Insect Biochem Mol Biol 38: 331345.
  39. Campbell CL, Keene KM, Brackney DE, Olson KE, Blair CD, Wilusz J, Foy BD, , 2008. Aedes aegypti uses RNA interference in defense against Sindbis virus infection. BMC Microbiol 8: 47.[Crossref]
  40. Xi Z, Ramirez JL, Dimopoulos G, , 2008. The Aedes aegypti toll pathway controls dengue virus infection. PLoS Pathog 4: e1000098.[Crossref]
  41. Platt KB, Linthicum KJ, Myint KS, Innis BL, Lerdthusnee K, Vaughn DW, , 1997. Impact of dengue virus infection on feeding behavior of Aedes aegypti . Am J Trop Med Hyg 57: 119125.
  42. Raikhel AS, Deitsch KW, Sappington TW, Crampton JM, Beard CB, Louis C, , 1997. Culture and analysis of the insect fat body. , eds. Molecular Biology of Insect Disease Vectors. London: Chapman Hall, 507522.[Crossref]
  43. Dimopoulos G, Richman A, Muller HM, Kafatos FC, , 1997. Molecular immune responses of the mosquito Anopheles gambiae to bacteria and malaria parasites. Proc Natl Acad Sci USA 94: 1150811513.[Crossref]
  44. Gorman MJ, Andreeva OV, Paskewitz SM, , 2000. Sp22D: a multidomain serine protease with a putative role in insect immunity. Gene 251: 917.[Crossref]
  45. Dimopoulos G, Muller HM, Levashina EA, Kafatos FC, , 2001. Innate immune defense against malaria infection in the mosquito. Curr Opin Immunol 13: 7988.[Crossref]
  46. Shi L, Paskewitz SM, , 2004. Identification and molecular characterization of two immune-responsive chitinase-like proteins from Anopheles gambiae . Insect Mol Biol 13: 387398.[Crossref]
  47. Shin SW, Kokoza V, Bian G, Cheon HM, Kim YJ, Raikhel AS, , 2005. REL1, a homologue of Drosophila dorsal, regulates toll antifungal immune pathway in the female mosquito Aedes aegypti . J Biol Chem 280: 1649916507.[Crossref]
  48. Cheon HM, Shin SW, Bian G, Park JH, Raikhel AS, , 2006. Regulation of lipid metabolism genes, lipid carrier protein lipophorin, and its receptor during immune challenge in the mosquito Aedes aegypti . J Biol Chem 281: 84268435.[Crossref]
  49. Cooper DM, Chamberlain CM, Lowenberger C, , 2009. Aedes FADD: a novel death domain-containing protein required for antibacterial immunity in the yellow fever mosquito, Aedes aegypti . Insect Biochem Mol Biol 39: 4754.[Crossref]
  50. Khin MM, Than KA, , 1983. Transovarial transmission of dengue 2 virus by Aedes aegypti in nature. Am J Trop Med Hyg 32: 590594.
  51. Rosen L, Shroyer DA, Tesh RB, Freier JE, Lien JC, , 1983. Transovarial transmission of dengue viruses by mosquitoes: Aedes albopictus and Aedes aegypti . Am J Trop Med Hyg 32: 11081119.
  52. Hull B, Tikasingh E, de Souza M, Martinez R, , 1984. Natural transovarial transmission of dengue 4 virus in Aedes aegypti in Trinidad. Am J Trop Med Hyg 33: 12481250.
  53. Freier JE, Rosen L, , 1987. Vertical transmission of dengue viruses by mosquitoes of the Aedes scutellaris group. Am J Trop Med Hyg 37: 640647.
  54. Freier JE, Rosen L, , 1988. Vertical transmission of dengue viruses by Aedes mediovittatus . Am J Trop Med Hyg 39: 218222.
  55. Mitchell CJ, Miller BR, , 1990. Vertical transmission of dengue viruses by strains of Aedes albopictus recently introduced into Brazil. J Am Mosq Control Assoc 6: 251253.
  56. Shroyer DA, , 1990. Vertical maintenance of dengue-1 virus in sequential generations of Aedes albopictus . J Am Mosq Control Assoc 6: 312314.
  57. Bosio CF, Thomas RE, Grimstad PR, Rai KS, , 1992. Variation in the efficiency of vertical transmission of dengue-1 virus by strains of Aedes albopictus (Diptera: Culicidae). J Med Entomol 29: 985989.[Crossref]
  58. Mourya DT, Gokhale MD, Basu A, Barde PV, Sapkal GN, Padbidri VS, Gore MM, , 2001. Horizontal and vertical transmission of dengue virus type 2 in highly and lowly susceptible strains of Aedes aegypti mosquitoes. Acta Virol 45: 6771.
  59. Joshi V, Mourya DT, Sharma RC, , 2002. Persistence of dengue-3 virus through transovarial transmission passage in successive generations of Aedes aegypti mosquitoes. Am J Trop Med Hyg 67: 158161.
  60. Thenmozhi V, Hiriyan JG, Tewari SC, Philip Samuel P, Paramasivan R, Rajendran R, Mani TR, Tyagi BK, , 2007. Natural vertical transmission of dengue virus in Aedes albopictus (Diptera: Culicidae) in Kerala, a southern Indian state. Jpn J Infect Dis 60: 245249.
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2010.10-0193
Loading
/content/journals/10.4269/ajtmh.2010.10-0193
Loading

Data & Media loading...

  • Received : 01 Apr 2010
  • Accepted : 22 Apr 2010

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error