Volume 83, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Fleas collected from rats during a three-year period (2000–2003) in 51 areas of all provinces of Cyprus were tested by molecular analysis to characterize the prevalence and identity of fleaborne rickettsiae. , the causative agent of murine typhus, was detected in (4%) and in (6.6%). was detected in (1%). This is the first report of in and from rats, in Cyprus, and the first report of in in Europe. The role of fleas (mainly ) was confirmed in the epidemiologic cycle of murine typhus in Cyprus by interrelation of current results with those of previous studies. The geographic distribution of fleas coincided with the geographic distribution of the pathogen they can harbor, which emphasizes the potential risk of flea-transmitted infections in Cyprus.


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  1. Azad AF, , 1990. Epidemiology of murine typhus. Annu Rev Entomol 35: 553569.[Crossref]
  2. Chaniotis B, Psarulaki A, Chaliotis G, Gozalo Garcia G, Gozadinos T, Tselentis Y, , 1994. Transmission cycle of murine typhus in Greece. Ann Trop Med Parasitol 88: 645647.[Crossref]
  3. Fergie JE, Purcell K, Wanat D, , 2000. Murine typhus in south Texas children. Pediatr Infect Dis J 19: 535538.[Crossref]
  4. Azad AF, Radulovic S, Higgins JA, Noden BH, Troyer JM, , 1997. Flea-borne rickettsioses: ecologic considerations. Emerg Infect Dis 3: 319327.[Crossref]
  5. Azad AF, David W, , 1988. Relationship of vector biology and epidemiology of louse and flea-borne rickettsioses. , ed. Biology of Rickettsial Diseases. Boca Raton, FL: CRC Press, Inc., 5161.
  6. Schriefer ME, Sacci JB, Jr Dumler JS, Bullen MG, Azad AF, , 1994. Identification of a novel rickettsial infection in a patient diagnosed with murine typhus. J Clin Microbiol 32: 949954.
  7. Adams JR, Schmidtmann ET, Azad AF, , 1990. Infection of colonized cat fleas, Ctenocephalides felis (Bouche), with a Rickettsia-like microorganism. Am J Trop Med Hyg 43: 400409.
  8. Parola P, Davoust B, Raoult D, , 2005. Tick- and flea-borne rickettsial emerging zoonoses. Vet Res 36: 469492.[Crossref]
  9. Renvoise A, Joliot AY, Raoult D, , 2009. Rickettsia felis infection in man, France. Emerg Infect Dis 15: 11261127.[Crossref]
  10. Perez-Osorio CE, Zavala-Velazquez JE, Arias Leon JJ, Zavala-Castro JE, , 2008. Rickettsia felis as emergent global threat for humans. Emerg Infect Dis 14: 10191023.[Crossref]
  11. Koliou M, Psaroulaki A, Georgiou C, Ioannou I, Tselentis Y, Gikas A, , 2007. Murine typhus in Cyprus: 21 paediatric cases. Eur J Clin Microbiol Infect Dis 26: 491493.[Crossref]
  12. Antoniou M, Psaroulaki A, Toumazos P, Mazeris A, Ioannou I, Papaprodromou M, Georgiou K, Christophi N, Patsias A, Loucaides F, Moschandreas J, Tsatsaris A, Tselentis Y, , 2010. Rats as indicators of the presence and dispersal of pathogens involved in zoonoses in Cyprus. Vector Borne Zoonotic Dis Apr 6 [Epub ahead of print].
  13. Horta MC, Chiebao DP, de Souza DB, Ferreira F, Pinheiro SR, Labruna MB, Schumaker TT, , 2006. Prevalence of Rickettsia felis in the fleas Ctenocephalides felis and Ctenocephalides canis from two Indian villages in Sao Paulo Municipality, Brazil. Ann N Y Acad Sci 1078: 361363.[Crossref]
  14. Roux V, Rydkina E, Eremeeva M, Raoult D, , 1997. Citrate synthase gene comparison, a new tool for phylogenetic analysis, and its application for the rickettsiae. Int J Syst Bacteriol 47: 252261.[Crossref]
  15. Roux V, Raoult D, , 2000. Phylogenetic analysis of members of the genus Rickettsia using the gene encoding the outer-membrane protein rOmpB (ompB). Int J Syst Evol Microbiol 50: 14491455.[Crossref]
  16. Psaroulaki A, Antoniou M, Papaeustathiou A, Toumazos P, Loukaides F, Tselentis Y, , 2006. First detection of Rickettsia felis in Ctenocephalides felis fleas parasitizing rats in Cyprus. Am J Trop Med Hyg 74: 120122.
  17. Bitam I, Parola P, De La Cruz KD, Matsumoto K, Baziz B, Rolain JM, Belkaid M, Raoult D, , 2006. First molecular detection of Rickettsia felis in fleas from Algeria. Am J Trop Med Hyg 74: 532535.
  18. Marquez FJ, Muniain MA, Rodriguez-Liebana JJ, Del Toro MD, Bernabeu-Wittel M, Pachon AJ, , 2006. Incidence and distribution pattern of Rickettsia felis in peridomestic fleas from Andalusia, southeast Spain. Ann N Y Acad Sci 1078: 344346.[Crossref]
  19. Venzal JM, Perez-Martinez L, Felix ML, Portillo A, Blanco JR, Oteo JA, , 2006. Prevalence of Rickettsia felis in Ctenocephalides felis and Ctenocephalides canis from Uruguay. Ann N Y Acad Sci 1078: 305308.[Crossref]
  20. Stevenson HL, Labruna MB, Montenieri JA, Kosoy MY, Gage KL, Walker DH, , 2005. Detection of Rickettsia felis in a New World flea species, Anomiopsyllus nudata (Siphonaptera: Ctenophthalmidae). J Med Entomol 42: 163167.[Crossref]
  21. De Sousa R, Edouard-Fournier P, Santos-Silva M, Amaro F, Bacellar F, Raoult D, , 2006. Molecular detection of Rickettsia felis, Rickettsia typhi and two genotypes closely related to Bartonella elizabethae . Am J Trop Med Hyg 75: 727731.
  22. Gilles J, Just FT, Silaghi C, Pradel I, Passos LM, Lengauer H, Hellmann K, Pfister K, , 2008. Rickettsia felis in fleas, Germany. Emerg Infect Dis 14: 12941296.[Crossref]
  23. Jiang J, Soeatmadji DW, Henry KM, Ratiwayanto S, Bangs MJ, Richards AL, , 2006. Rickettsia felis in Xenopsylla cheopis, Java, Indonesia. Emerg Infect Dis 12: 12811283.[Crossref]
  24. Loftis AD, Reeves WK, Szumlas DE, Abbassy MM, Helmy IM, Moriarity JR, Dasch GA, , 2006. Surveillance of Egyptian fleas for agents of public health significance: Anaplasma, Bartonella, Coxiella, Ehrlichia, Rickettsia, and Yersinia pestis . Am J Trop Med Hyg 75: 4148.
  25. Azad AF, Traub R, , 1987. Transmission of murine typhus rickettsiae by Leptopsylla segnis (Siphonaptera: Leptopsyllidae). J Med Entomol 24: 689693.[Crossref]
  26. Tselentis Y, Psaroulaki A, Maniatis J, Spyridaki I, Babalis T, , 1996. Genotypic identification of murine typhus Rickettsia in rats and their fleas in an endemic area of Greece by the polymerase chain reaction and restriction fragment length polymorphism. Am J Trop Med Hyg 54: 413417.
  27. Raoult D, La Scola B, Enea M, Fournier PE, Roux V, Fenollar F, Galvao MA, de Lamballerie X, , 2001. A flea-associated Rickettsia pathogenic for humans. Emerg Infect Dis 7: 7381.[Crossref]
  28. Znazen A, Rolain JM, Hammami A, Jemaa MB, Raoult D, , 2006. Rickettsia felis infection, Tunisia. Emerg Infect Dis 12: 138140.[Crossref]

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  • Received : 23 Feb 2010
  • Accepted : 14 Jul 2010

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