Volume 83, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Morphological differentiation of mosquitoes in the subgenera () and () in Guatemala is difficult, with reliable identification ensured only through examination of larval skins from individually reared specimens and associated male genitalia. We developed a multiplexed polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay to identify common () and (). () () , () , () , () / () , and () were identified directly with a multiplexed primer cocktail comprising a conserved forward primer and specific reverse primers targeting ribosomal DNA (rDNA). and were differentiated by restriction digest of homologous amplicons. The assay was developed and optimized using well-characterized specimens from Guatemala and the United States and field tested with unknown material from Guatemala. This assay will be a valuable tool for mosquito identification in entomological and arbovirus ecology studies in Guatemala.


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  • Received : 05 Feb 2010
  • Accepted : 22 Apr 2010
  • Published online : 05 Aug 2010

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