Volume 83, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Most rapid diagnostic tests (RDTs) available use histidine-rich protein 2 (HRP2) as a target. However, it has been reported that sequence variations of this protein affects its sensitivity. Currently, there is insufficient evidence for HRP2 variability in isolates from Colombia and its relationship with RDT performance. To determine possible geographic differences and their effects on the performance of RDTs, 22 blood samples from patients with . malaria from Tumaco and Buenaventura, Colombia were assessed by measurement of HRP2 concentration by an HRP2 enzyme-linked immunosorbent assay, RDTs, and thick blood smear. Statistical analysis showed an association between RDT performance and HRP2 concentrations. No significant difference was found between locations. A large variation of antigen concentration in samples was found at same parasitemia. In contrast to previously reports, there was no correlation between initial parasitemia and HRP2 concentration. Our results indicate that antigen quantity should be studied more carefully because the sensitivity of the RDT is affected more by antigen concentration than by parasitemia.


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  1. SIVIGILA, 2007. Informe Final 2007 Enfermedades Transmitidas por Vectores. Cali, Colombia: SIVIGILA.
  2. World Health Organization, 2009. World Malaria Report 2008. Available at: www.who.com. Accessed July 16, 2010.
  3. WHO/USAID/DFID/AusAID, 2003. Malaria Rapid Diagnosis—Making It Work. Geneva: World Health Organization.
  4. Organización Panamericana de la Salud, Red Amazónica de Vigilancia de la Resistencia a las Antimaláricos, 2005. Investigación Operacional sobre la Implementación del Uso de Pruebas Rápidas de Diagnóstico de Malaria. Guayaquil, Ecuador: Organización Panamericana de La Salud.
  5. World Health Organization, 2005. List of Known Commercially-Available Antigen-Detecting Malaria RDTs. Geneva: World Health Organization.
  6. Lee N, Baker J, Andrews K, Gatton M, Bell D, Cheng Q, McCarthy J, , 2006. Effect of sequence variation in Plasmodium falciparum histidine-rich protein 2 on binding of specific monoclonal antibodies: implications for rapid diagnostic tests for malaria. J Clin Microbiol 44: 27732778.[Crossref]
  7. Cellabs, 2004. Malaria Ag ELISA. Available at: http://www.tcsbiosciences.co.uk/downloads/CellabsMalariaELISA.pdf. Accessed July 16, 2010.
  8. World Health Organization, 2006. Training in Tropical Diseases Methods Manual for Laboratory Quality Control Testing of Malaria RDTs. SOP. 3.08. Geneva: World Health Organization.
  9. Earle WC, Pérez M, , 1932. Enumeration of parasites in the blood of malaria patients. J Lab Clin Med 11: 11241130.
  10. Span Diagnostics Ltd., 2007. Parahit Rapid Diagnostic Test. Surat, India: Span Diagnostics Ltd.
  11. Bradford M, , 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248254.[Crossref]
  12. Baker J, McCarthy J, Gatton M, Kyle D, Belizario V, Luchavez J, Bell D, Cheng Q, , 2005. Genetic diversity of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and its effect on the performance of PfHRP2-based rapid diagnostic tests. J Infect Dis 192: 870877.[Crossref]
  13. Desakorn V, Dondorp A, Silamut K, Pongtavornpinyo W, Sahassananda D, Chotivanich K, Pitisuttithum P, Smithyman A, Day N, White N, , 2005. Stage-dependent production and release of histidine-rich protein 2 by Plasmodium falciparum . Trans R Soc Trop Med Hyg 99: 517524.[Crossref]
  14. Kifude C, Rajasekariah H, Sullivan DJ, Stewart V, Angov E, Martin S, Diggs C, Waitumbi J, , 2008. Enzyme-linked immunosorbent assay for detection of Plasmodium falciparum histidine-rich protein 2 in blood, plasma, and serum. Clin Vaccine Immunol 15: 10121018.[Crossref]

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Supplementary Data


  • Received : 03 Feb 2010
  • Accepted : 02 Jul 2010

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