Volume 83, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Multiplex realtime polymerase chain reaction (PCR) was developed for differential detection of , , and . Specific primers were designed for all three species, and then differentiation of and was achieved simultaneously using a hybridization probe and melting curve analysis, whereas was detected with a separate probe under the same condition. This assay detected as little as 0.2 pg of DNA and 2 pg each for and DNA. Thirty-five clinical samples suspected to be infection by microscopy were tested. The results showed 32 positive samples; four samples were . and 28 samples were . Interestingly, one positive sample showed a mixed infection with . This is the first report of infection from Thailand and this assay is currently the most rapid and sensitive method to differentiate these human amoebas.


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  • Received : 24 Jan 2010
  • Accepted : 27 May 2010

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