Volume 83, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The combined efficacy of a long-lasting insecticidal net (LLIN) and a carbamate-treated plastic sheeting (CTPS) or indoor residual spraying (IRS) for control of insecticide-resistant mosquitoes was evaluated in experimental huts in Burkina Faso. from the area is resistant to pyrethroids and to a lesser extent, carbamates. Relatively low mortality rates were observed with the LLIN (44%), IRS (42%), and CTPS (52%), whereas both combinations killed significantly more mosquitoes (~70% for LLIN + CTPS and LLIN + IRS). Blood feeding by was uninhibited by IRS and CTPS compared with LLIN (43%), LLIN + CTPS (58%), and LLIN + IRS (56%). No evidence for selection of the and alleles was observed with the combinations, whereas a survival advantage of mosquitoes bearing the mutation was observed with IRS and CTPS. The results suggest that the combination of the two interventions constitutes a potential tool for vector-resistance management.


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  1. World Health Organization, 2008. World Malaria Report 2008. Geneva, Switzerland: WHO.
  2. World Health Organization, 2006. Pesticides and Their Application for the Control of Vectors and Pests of Public Health Importance. WHO.
  3. Lengeler C, , 2004. Insecticide-treated bed nets and curtains for preventing malaria. Cochrane Database Syst Rev CD000363.
  4. Kolaczinski K, Kolaczinski J, Kilian A, Meek S, , 2007. Extension of indoor residual spraying for malaria control into high transmission settings in Africa. Trans R Soc Trop Med Hyg 101: 852853.[Crossref]
  5. Yukich JO, Lengeler C, Tediosi F, Brown N, Mulligan JA, Stevens DC, Justino J, Conteh L, Maharaj R, Erskine M, Mueller DH, Wiseman V, Ghebremeskel T, Zerom M, Goodman C, McGuire D, Urrutia JM, Sakho F, Hanson K, Sharp B, , 2008. Costs and consequences of large-scale vector control for malaria. Malar J 7: 258.[Crossref]
  6. Zaim M, Aitio A, Nakashima N, , 2000. Safety of pyrethroid-treated mosquito nets. Med Vet Entomol 14: 15.[Crossref]
  7. Santolamazza F, Calzetta M, Etang J, Barrese E, Dia I, Caccone A, Donnelly MJ, Petrarca V, Simard F, Pinto J, della Torre A, , 2008. Distribution of knock-down resistance mutations in Anopheles gambiae molecular forms in west and west-central Africa. Malar J 7: 74.[Crossref]
  8. Henry MC, Assi SB, Rogier C, Dossou-Yovo J, Chandre F, Guillet P, Carnevale P, , 2005. Protective efficacy of lambda-cyhalothrin treated nets in Anopheles gambiae pyrethroid resistance areas of Cote d'Ivoire. Am J Trop Med Hyg 73: 859864.
  9. N'Guessan R, Corbel V, Akogbeto M, Rowland M, , 2007. Reduced efficacy of insecticide-treated nets and indoor residual spraying for malaria control in pyrethroid resistance area, Benin. Emerg Infect Dis 13: 199206.[Crossref]
  10. Guillet P, N'Guessan R, Darriet F, Traore-Lamizana M, Chandre F, Carnevale P, , 2001. Combined pyrethroid and carbamate ‘two-in-one’ treated mosquito nets: field efficacy against pyrethroid-resistant Anopheles gambiae and Culex quinquefasciatus . Med Vet Entomol 15: 105112.[Crossref]
  11. Curtis CF, Mnzava AE, , 2000. Comparison of house spraying and insecticide-treated nets for malaria control. Bull World Health Organ 78: 13891400.
  12. Miller JE, Lindsay SW, Armstrong JR, , 1991. Experimental hut trials of bednets impregnated with synthetic pyrethroid or organophosphate insecticide for mosquito control in The Gambia. Med Vet Entomol 5: 465476.[Crossref]
  13. Djènontin A, Chabi J, Baldet T, Irish S, Pennetier C, Hougard J-M, Corbel V, Akogbéto M, Chandre F, , 2009. Managing insecticide resistance in malaria vectors by combining carbamate-treated plastic wall sheeting and pyrethroid-treated bed nets. Malar J 8: 233.[Crossref]
  14. Dabire KR, Diabate A, Djogbenou L, Ouari A, N'Guessan R, Ouedraogo JB, Hougard JM, Chandre F, Baldet T, , 2008. Dynamics of multiple insecticide resistance in the malaria vector Anopheles gambiae in a rice growing area in south-western Burkina Faso. Malar J 7: 188.[Crossref]
  15. Pennetier C, Costantini C, Corbel V, Licciardi S, Dabire RK, Lapied B, Chandre F, Hougard JM, , 2008. Mixture for controlling insecticide-resistant malaria vectors. Emerg Infect Dis 14: 17071714.[Crossref]
  16. World Health Organization, 2005. Guideline for Laboratory and Field Testing of Long Lasting Mosquito Nets. Geneva, Switzerland: WHO.
  17. World Health Organization, 2006. Guidelines for Testing Mosquito Adulticides Intended for Indoor Residual Spraying (IRS) and Insecticide Treated Nets (ITNs). Geneva, Switzerland: WHO.
  18. Scott JA, Brogdon WG, Collins FH, , 1993. Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49: 520529.
  19. Favia G, Lanfrancotti A, Spanos L, Siden Kiamos I, Louis C, , 2001. Molecular characterization of ribosomal DNA polymorphisms discriminating among chromosomal forms of Anopheles gambiae s.s. Insect Mol Biol 10: 1923.[Crossref]
  20. Martinez Torres D, Chandre F, Williamson MS, Darriet F, Berge JB, Devonshire AL, Guillet P, Pasteur N, Pauron D, , 1998. Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s. Insect Mol Biol 7: 179184.[Crossref]
  21. Weill M, Malcolm C, Chandre F, Mogensen K, Berthomieu A, Marquine M, Raymond M, , 2004. The unique mutation in ace-1 giving high insecticide resistance is easily detectable in mosquito vectors. Insect Mol Biol 13: 17.[Crossref]
  22. Addinsoft, 2009. XLSTAT, Data Analysis and Statistics with Microsoft Excel. Paris, France: Addinsoft.
  23. Raymond M, Rousset F, , 1995. GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86: 248249.[Crossref]
  24. Curtis CF, Myamba J, Wilkes TJ, , 1996. Comparison of different insecticides and fabrics for anti-mosquito bednets and curtains. Med Vet Entomol 10: 111.[Crossref]
  25. Hougard JM, Corbel V, N'Guessan R, Darriet F, Chandre F, Akogbeto M, Baldet T, Guillet P, Carnevale P, Traore-Lamizana M, , 2003. Efficacy of mosquito nets treated with insecticide mixtures or mosaics against insecticide resistant Anopheles gambiae and Culex quinquefasciatus (Diptera: Culicidae) in Cote d'Ivoire. Bull Entomol Res 93: 491498.[Crossref]
  26. Ansari MA, Razdan RK, , 2004. Impact of residual spraying of bendiocarb against the malaria vector Anopheles culicifaces in selected villages of the Ghaziabad District, Uttar Pradest, India. J Am Mosq Control Assoc 20: 418423.
  27. Mpofu SM, Kanyimo KH, Masendu H, , 1991. Potentia use of bendiocarb (FICAM VC) for malaria control in area of Zimbabwe. J Am Mosq Control Assoc 7: 536542.
  28. N'Guessan R, Darriet F, Doannio JM, Chandre F, Carnevale P, , 2001. Olyset Net efficacy against pyrethroid-resistant Anopheles gambiae and Culex quinquefasciatus after 3 years' field use in Côte d'Ivoire. Med Vet Entomol 15: 97104.[Crossref]
  29. World Health Organization, 2009. Report of the Twelfth WHOPES Working Group Meeting. Geneva, Switzerland: WHO.
  30. President Malaria Initiative, 2009. Third Annual Report. Atlanta, GA: President Malaria Initiative.
  31. Corbel V, Hougard JM, N'Guessan R, Chandre F, , 2003. Evidence for selection of insecticide resistance due to insensitive acetylcholinesterase by carbamate-treated nets in Anopheles gambiae s.s. (Diptera: Culicidae) from Cote d'Ivoire. J Med Entomol 40: 985988.[Crossref]
  32. Bill and Melinda Gates Foundation, 2007. Market Assessment for Public Health Pesticide Products, a Report by the Bill and Melinda Gates Foundation and the Boston Consulting Group. Washington, DC: Bill and Melinda Gates Foundation.
  33. N'Guessan R, Boko P, Odjo A, Knols B, Akogbeto M, Rowland M, , 2009. Control of pyrethroid-resistant Anopheles gambiae and Culex quinquefasciatus mosquitoes with chlorfenapyr in Benin. Trop Med Int Health 14: 389395.[Crossref]
  34. N'Guessan R, Corbel V, Bonnet J, Yates A, Asidi A, Boko P, Odjo A, Akogbeto M, Rowland M, , 2007. Evaluation of indoxacarb, an oxadiazine insecticide for the control of pyrethroid-resistant Anopheles gambiae (Diptera: Culicidae). J Med Entomol 44: 270276.[Crossref]
  35. Sharp BL, Ridl FC, Govender D, Kuklinski J, Kleinschmidt I, , 2007. Malaria vector control by indoor residual insecticide spraying on the tropical island of Bioko, Equatorial Guinea. Malar J 6: 52.[Crossref]
  36. Van Bortel W, Chinh VD, Berkvens D, Speybroeck N, Trung HD, Coosmans M, , 2009. Impact of insecticide treated nets on wild pyrethroid resistant Anopheles epiroticus population from southern Vietnam tested in experimental huts. Malar J 8: 248.[Crossref]

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  • Received : 07 Jan 2010
  • Accepted : 25 Feb 2010

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