1921
Volume 83, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We identified and followed-up for two years rodents infected with genotypes by using xenodiagnosis with two vector species ( and ), polymerase chain reaction DNA-based detection of insect dejections, Southern blot analysis, and minicircle hybridization with genotype-specific probes. Results show temporal fluctuations of infection with four parasite lineages (TCI, TCII, TCV, and TCVI) in one co-infected . Results are discussed in the context of parasitemia level and infection control in mammal hosts.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2010.09-0797
2010-08-05
2017-09-26
Loading full text...

Full text loading...

/deliver/fulltext/14761645/83/2/380.html?itemId=/content/journals/10.4269/ajtmh.2010.09-0797&mimeType=html&fmt=ahah

References

  1. Zingales B, Andrade SG, Briones MR, Campbell DA, Chiari E, Fernandes O, Guhl F, Lages-Silva E, Macedo AM, Machado CR, Miles MA, Romanha AJ, Sturm NR, Tibayrenc M, Schijman AG, , 2009. A new consensus for Trypanosoma cruzi intraspecific nomenclature: second revision meeting recommends TcI to TcVI. Mem Inst Oswaldo Cruz 104: 10511054.[Crossref]
  2. Tibayrenc M, , 1998. Genetic epidemiology of parasitic protozoa and other infections agents: the need for an integrated approach. Int J Parasitol 28: 85104.[Crossref]
  3. Coronado X, Zulantay I, Albrecht H, Rozas M, Apt W, Ortiz S, Rodriguez J, Sanchez G, Solari A, , 2006. Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines: implications in the molecular epidemiology of Chile. Am J Trop Med Hyg 74: 10081012.
  4. Rozas M, Botto-Mahan C, Coronado X, Ortiz S, Cattan PE, Solari A, , 2007. Coexistence of Trypanosoma cruzi genotypes in wild and peridomestic mammals in Chile. Am J Trop Med Hyg 77: 647653.
  5. Coronado X, Rozas M, Botto-Maham C, Ortiz S, Cattan P, Solari A, , 2009. Molecular epidemiology of Chagas disease in the wild transmission cycle: the evaluation in the sylvatic vector Mepraia spinolai from an endemic area of Chile. Am J Trop Med Hyg 81: 656659.[Crossref]
  6. Dias E, , 1940. Xenodiagnosticos seriados em caes infectados con amostras venezuelanas de Schizotrypanum cruzi . Bras Med 54: 859861.
  7. Schenone H, Contreras MC, Rojas A, , 1991. Yielding of xenodiagnosis, according to the number of boxes used in 1,181 persons with chronic chagasic infection diagnosed with indirect hemagglutination reaction. Bol Chil Parasitol 46: 5861.
  8. Westenberger SJ, Barnabé C, Campbell DA, Sturm NR, , 2005. Two hybridization events define the population structure of Trypanosoma cruzi . Genetics 171: 527543.[Crossref]
  9. Campos R, Acuña-Retamar M, Botto-Mahan C, Ortiz S, Cattan PE, Solari A, , 2007. Susceptibility of Mepraia spinolai and Triatoma infestans to different Trypanosoma cruzi strains from naturally infected rodent hosts. Acta Trop 104: 2529.[Crossref]
  10. Veas F, Breniere SF, Cuny G, Brengues C, Solari A, Tibayrenc M, , 1991. General procedure to construct highly specific kDNA probes for clones of Trypanosoma cruzi for sensitive detection by polymerase chain reaction. Cell Mol Biol 37: 7384.
  11. Lane JE, Ribeiro-Rodriguez R, Olivares-Villagomez D, Vnencak-Jones CL, McCurley T, Carter CE, , 2003. Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction. Mem Inst Oswaldo Cruz 98: 373376.[Crossref]
  12. de Lana M, da Silveira Pinto A, Barnabe C, Quesney V, Noel S, Tibayrenc M, , 1998. Trypanosoma cruzi: compared vectorial transmissibility of three major clonal genotypes by Triatoma infestans . Exp Parasitol 90: 2025.[Crossref]
  13. Vago AR, Andrade LO, Leite AA, d`Avila Reis D, Macedo AM, Adad SJ, Tostes S, Jr Moreira MC, Filho GB, Pena SD, , 2000. Genetic characterization of Trypanosoma cruzi directly from tissues of patients with chronic Chagas disease: differential distribution of genetic types into diverse organs. Am J Pathol 156: 18051809.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2010.09-0797
Loading
/content/journals/10.4269/ajtmh.2010.09-0797
Loading

Data & Media loading...

  • Received : 29 Dec 2009
  • Accepted : 19 Apr 2010

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error