1921
Volume 83, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

This study was performed to determine the nationwide antigenic diversity of in South Korea. Sequence analysis was performed around variable domains I and II of a 56-kDa protein-encoding gene. We used eschar to overcome the disadvantages of conventional serotyping. The serological passive hemagglutination assay (PHA) was assessed based on the genotyping results. We analyzed 153 isolates from scrub typhus patients in major endemic areas and found that Boryong was the major strain (68.6%). New strains were also identified: Taguchi (19.6%), Kanda/Kawasaki (9.2%), and UAP7 (1.3%). PHA yielded significantly fewer positive results among Kawasaki strains ( < 0.001), which are not included in the PHA antigen panel. In South Korea, Boryong was still the predominant strain, but the sequence analysis identified new changes in minor strains (30.1%). This antigenic drift had a negative effect on the PHA results. Periodic surveillance of the contemporary strains using sequence analysis is needed.

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References

  1. Kim DM, Kim HL, Park CY, Yang TY, Lee JH, Yang JT, Shim SK, Lee SH, , 2006. Clinical usefulness of eschar polymerase chain reaction for the diagnosis of scrub typhus: a prospective study. Clin Infect Dis 43: 12961300.[Crossref]
  2. Kim DM, Yun NR, Yang TY, Lee JH, Yang JT, Choi EN, Park MY, Lee SH, , 2006. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: a prospective study. Am J Trop Med Hyg 75: 542545.
  3. Murai K, Okayama A, Horinouchi H, Oshikawa T, Tachibana N, Tsubouchi H, , 1995. Eradication of Rickettsia tsutsugamushi from patients' blood by chemotherapy as assessed by the polymerase chain reaction. Am J Trop Med Hyg 52: 325327.
  4. Kelly DJ, Fuerst PA, Ching WM, Richards AL, , 2009. Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientia tsutsugamushi . Clin Infect Dis 48 (Suppl 3): S203S230.[Crossref]
  5. Stover CK, Marana DP, Carter JM, Roe BA, Mardis E, Oaks EV, , 1990. The 56-kilodalton major protein antigen of Rickettsia tsutsugamushi: molecular cloning and sequence analysis of the sta56 gene and precise identification of a strain-specific epitope. Infect Immun 58: 20762084.
  6. Furuya Y, Yoshida Y, Katayama T, Kawamori F, Yamamoto S, Ohashi N, Tamura A, Kawamura A, Jr, 1991. Specific amplification of Rickettsia tsutsugamushi DNA from clinical specimens by polymerase chain reaction. J Clin Microbiol 29: 26282630.
  7. Furuya Y, Yoshida Y, Katayama Y, Yamamoto S, Kawamura A, , 1993. Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction. J Clin Microbiol 31: 16371640.
  8. Lee JS, Ahn C, Kim YK, Lee MH, , 1986. Thirteen cases of rickettsial infection including 9 cases of tsutsugamushi disease first confirmed in Korea [Korean]. J Korean Med Assoc 29: 430438.
  9. Kweon SS, Choi JS, Lim HS, Kim JR, Kim KY, Ryu SY, Yoo HS, Park O, , 2009. Rapid increase of scrub typhus, South Korea, 2001–2006. Emerg Infect Dis 15: 11271129.[Crossref]
  10. Ohashi N, Nashimoto H, Ikeda H, Tamura A, , 1992. Diversity of immunodominant 56-kDa type-specific antigen (TSA) of Rickettsia tsutsugamushi: sequence and comparative analyses of the genes encoding TSA homologues from four antigenic variants. J Biol Chem 267: 1272812735.
  11. Enatsu T, Urakami H, Tamura A, , 1999. Phylogenetic analysis of Orientia tsutsugamushi strains based on the sequence homologies of 56-kDa type-specific antigen genes. FEMS Microbiol Lett 180: 163169.[Crossref]
  12. Tamura A, Yamamoto N, Koyama S, Makisaka Y, Takahashi M, Urabe K, Takaoka M, Nakazawa K, Urakami H, Fukuhara M, , 2001. Epidemiological survey of Orientia tsutsugamushi distribution in field rodents in Saitama prefecture, Japan, and discovery of a new type. Microbiol Immunol 45: 439446.[Crossref]
  13. Thompson JD, Higgins DG, Gibson TJ, , 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 46734680.[Crossref]
  14. Saitou N, Nei M, , 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406425.
  15. Felsenstein J, , 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783791.[Crossref]
  16. Ohashi N, Nashimoto H, Ikeda H, Tamura A, , 1990. Cloning and sequencing of the gene (tsg56) encoding a type-specific antigen from Rickettsia tsutsugamushi . Gene 91: 119122.[Crossref]
  17. Kim IS, Seong SY, Woo SG, Choi MS, Chang WH, , 1993. High-level expression of a 56-kilodalton protein gene (bor56) of Rickettsia tsutsugamushi Boryong and its application to enzyme-linked immunosorbent assays. J Clin Microbiol 31: 598605.
  18. Kang JS, Chang WH, , 1999. Antigenic relationship among the eight prototype and new serotype strains of Orientia tsutsugamushi revealed by monoclonal antibodies. Microbiol Immunol 43: 229234.[Crossref]
  19. Ohashi N, Koyama Y, Urakami H, Fukuhara M, Tamura A, Kawamori F, Yamamoto S, Kasuya S, Yoshimura K, , 1996. Demonstration of antigenic and genotypic variation of Orientia tsutsugamushi which were isolated in Japan, and their classification into type and subtype. Microbiol Immunol 40: 627638.[Crossref]
  20. Chang WH, , 1994. Tsutsugamushi Disease in Korea. Seoul, Korea: Seohung Press, 2530.
  21. Korea Centers for Disease Control and Prevention, 2004. Current trends of scrub typhus (Korean). Commun Dis Monthly Rep 15: 245252.
  22. Ree HI, Kim TE, Lee IY, Jeon SH, Hwang UW, Chang WH, , 2001. Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction. Am J Trop Med Hyg 65: 528534.
  23. Shim SK, Choi EN, Yu KO, Park HJ, Kim CM, Lee KH, Park JK, Park PH, Yoon MH, Park SH, Choi YS, Hwang KJ, Park MY, , 2009. Characterisation of Orientia tsutsugamushi genotypes from wild rodents and chigger mites in Korea. Clin Microbiol Infect 15: 311312.[Crossref]
  24. Liu YX, Feng D, Suo JJ, Xing YB, Liu G, Liu LH, Xiao HJ, Jia N, Gao Y, Yang H, Zuo SQ, Zhang PH, Zhao ZT, Min JS, Feng PT, Ma SB, Liang S, Cao WC, , 2009. Clinical characteristics of the autumn-winter type scrub typhus cases in south of Shandong province, northern China. BMC Infect Dis 9: 82.[Crossref]
  25. Ogawa M, Hagiwara T, Kishimoto T, Shiga S, Yoshida Y, Furuya Y, Kaiho I, Ito T, Nemoto H, Yamamoto N, Masukawa K, , 2002. Scrub typhus in Japan: epidemiology and clinical features of cases reported in 1998. Am J Trop Med Hyg 67: 162165.
  26. Kim DM, Yun NR, Yang TY, Lee JH, Yang JT, Shim SK, Choi EN, Park MY, Lee SH, , 2006. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: a prospective study. Am J Trop Med Hyg 75: 542545.
  27. Fournier PE, Siritantikorn S, Rolain JM, Suputtamongkol Y, Hoontrakul S, Charoenwat S, Losuwanaluk K, Parola P, Raoult D, , 2008. Detection of new genotypes of Orientia tsutsugamushi infecting humans in Thailand. Clin Microbiol Infect 14: 168173.[Crossref]
  28. Blacksell SD, Bryant NJ, Paris DH, Doust JA, Sakoda Y, Day NP, , 2007. Scrub typhus serologic testing with the indirect immunofluorescence method as a diagnostic gold standard: a lack of consensus leads to a lot of confusion. Clin Infect Dis 44: 391401.[Crossref]
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  • Received : 28 Dec 2009
  • Accepted : 30 Jun 2010

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