1921
Volume 82, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The immunoreactivity of EchiTAb-Plus-ICP, an antivenom developed for the treatment of snakebite envenoming in sub-Saharan Africa, to venoms of seven and species, was assessed by “antivenomics.” This proteomic approach is based on the ability of an antivenom to immunodeplete homologous or heterologous venom proteins. Our results show an extensive cross-reactivity of this antivenom against all and venoms studied, as revealed by the complete immunodepletion of the majority of venom components, including metalloproteinases, serine proteinases, C-type lectin-like proteins, some phospholipases A and L-amino acid oxidase. However, some phospholipases A, disintegrins and proteinase inhibitors were immunodepleted to only a partial extent. These results support the hypothesis that immunizing horses with a mixture of the venoms of , , and generates antibodies capable of recognizing the majority of components of medically-relevant homologous and heterologous viperid venoms of the genera and from sub-Saharan Africa.

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2017-11-20
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  • Received : 07 Dec 2009
  • Accepted : 13 Feb 2010

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