1921
Volume 82, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Rats are known to be the most important reservoirs and transmission sources of leptospirosis. However, the status of leptospirosis in the Philippines regarding reservoirs and transmission remains unknown. A survey was conducted in Metro Manila and Laguna that analyzed samples obtained from 106 rats. Using the microscopic agglutination test, we found that 92% of rat serum samples were positive for anti- antibodies; the most common infecting serovars were Manilae, Hebdomadis, and Losbanos. On the basis of pulsed-field gel electrophoresis and gyrase B gene sequence analyses, four groups of rat kidney isolates were found: serovar Manilae, serovar Losbanos, and serogroup Grippotyphosa, and serogroup Javanica. Most isolates were lethal after experimental infection of golden Syrian hamsters. Results showed that these four serovars and serogroups are circulating among rats, and that these animals may be one of the possible transmission sources of leptospirosis in the Philippines.

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References

  1. Famatiga EG, Topacio TM, Suva MH, Oliveros FM, , 1972. Studies on leptospirosis in animals and man in the Philippines V. Serological survey of leptospirosis among occupationally exposed Filipinos. Southeast Asian J Trop Med Public Health 3: 482487.
  2. World Health Organization, 2003. Human Leptospirosis: Guidance for Diagnosis, Surveillance and Control. Geneva: World Health Organization and International Leptospirosis Society.
  3. Faine S, Adler B, Bolin C, Perolat P, , 1999. Leptospira and Leptospirosis. Melbourne, Australia: MediSci.
  4. Wisseman CL, Jr Traub R, Gochenour WS, Jr Smadel JE, Lancaster WE, , 1955. Leptospirosis of man and animals in urban, rural and jungle areas of southeast Asia. Am J Trop Med Hyg 4: 2940.
  5. Levett PN, , 2004. Leptospirosis: a forgotten zoonosis? Clin Appl Immunol Rev 4: 435448.[Crossref]
  6. Cerquiera GM, Picardeau M, , 2009. A century of Leptospira strain typing. Infect Genet Evol 9: 760768.[Crossref]
  7. Herrmann JL, Bellenger E, Perolat P, Baranton G, Saint Girons I, , 1992. Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA: a new rapid method of serovar identification. J Clin Microbiol 30: 16961702.
  8. Carlos ER, Kundin WD, Tsai CC, Watten RH, Irving GS, Villanueva C, , 1971. Leptospirosis in the Philippines. VII. Serologic and isolation studies on horses. Southeast Asian J Trop Med Public Health 2: 151152.
  9. Famatiga EG, , 1973. Leptospirosis in Philippine monkeys. Southeast Asian J Trop Med Public Health 4: 316318.
  10. Padre LP, Watt G, Tuazon ML, Gray MR, Laughlin LW, , 1988. A serologic survey of rice-field leptospirosis in Central Luzon, Philippines. Southeast Asian J Trop Med Public Health 19: 197199.
  11. Masuzawa T, Dancel LA, Miyake M, Yanagihara Y, , 2001. Serological analysis of human leptospirosis in the Philippines. Microbiol Immunol 45: 9395.[Crossref]
  12. Yanagihara Y, Villanueva SY, Yoshida S, Okamoto Y, Masuzawa T, , 2007. Current status of leptospirosis in Japan and Philippines. Comp Immunol Microbiol Infect Dis 30: 399413.[Crossref]
  13. Famatiga EG, , 1971. Leptospira study on fevers of unknown origin in the Philippines. Southeast Asian J Trop Med Public Health 2: 153163.
  14. Basaca-Sevilla V, Cross JH, Alcantara A, Barrito B, Pastrana E, Sevilla J, Balagot R, Dungca S, , 1981. An outbreak of leptospirosis in an agricultural penal colony in the Philippines. Phil J Microbiol Infect Dis 10: 7382.
  15. Carlos ER, Kundin WD, Watten RH, Tsai CC, Irving GS, Carlos ET, Directo AC, Castillo LP, Gaitmaitan O, , 1970. Leptospirosis in the Philippines VI. Serologic and isolation studies on carabaos. Southeast Asian J Trop Med Public Health 1: 481482.
  16. Carlos ER, Kundin WD, Watten RH, Tsai CC, Irving GS, Carlos ET, Directo AC, , 1971. Leptospirosis in the Philippines: canine studies. Am J Vet Res 32: 14511454.
  17. Agudelo-Florez P, Londono AF, Quiroz VH, Angel JC, Moreno N, Loaiza ET, Munoz LF, Rodas JD, , 2009. Prevalence of Leptospira spp. in urban rodents from a groceries trade center of Medellin, Colombia. Am J Trop Med Hyg 81: 906910.[Crossref]
  18. Andrade J, Brandao AP, , 1987. Epidemiology of human leptospirosis, with special reference to greater Rio de Janeiro, Brazil, from 1970 to 1982. Mem Inst Oswaldo Cruz 82: 91100.[Crossref]
  19. Aragon PR, Famatiga EG, , 1965. Studies on leptospirosis. II. Laboratory evidence of human infection. Acta Med Philipp 1: 196198.
  20. Arambulo PV III, Topacio TM, Jr Famatiga EG, Sarmiento RV, Lopez S, , 1972. Leptospirosis among abattoir employees, dog pound workers, and fish inspectors in the city of Manila. Southeast Asian J Trop Med Public Health 3: 212220.
  21. Basaca-Sevilla V, Cross JH, Pastrana E, , 1986. Leptospirosis in the Philippines. Southeast Asian J Trop Med Public Health 17: 7174.
  22. Ciceroni L, Ciarrocchi S, Ciervo A, Petrucca A, Pinto A, Calderaro A, Viani I, Galati L, Dettori G, Chezzi C, , 2002. Differentiation of leptospires of the serogroup Pomona by monoclonal antibodies, pulsed-field gel electrophoresis and arbitrarily primed polymerase chain reaction. Res Microbiol 153: 3744.[Crossref]
  23. Ciceroni L, Lombardo D, Pinto A, Ciarrocchi S, Simeoni J, , 2000. Prevalence of antibodies to Leptospira serovars in sheep and goats in Alto Adige-South Tyrol. J Vet Med B Infect Dis Vet Public Health 47: 217223.[Crossref]
  24. Ciceroni L, Pinto A, Benedetti E, Pizzocaro P, Lupidi R, Cinco M, Gelosa L, Grillo R, Rondinella V, Maruccio L, Mansuet S, Ioli A, Franzin L, Giannico F, Cacciapuoti B, , 1995. Human leptospirosis in Italy, 1986–1993. Eur J Epidemiol 11: 707710.[Crossref]
  25. Fresh JW, Tsai CC, Lai CH, Chang CT, , 1968. Leptospirosis in man and rodents in Taiwan. Am J Trop Med Hyg 17: 760768.
  26. Kawabata H, Sakakibara S, Imai Y, Masuzawa T, Fujita H, Tsurumi M, Sato F, Takano A, Nogami S, Kaneda K, Watanabe H, , 2006. First record of Leptospira borgpetersenii isolation in the Amami Islands, Japan. Microbiol Immunol 50: 429434.[Crossref]
  27. Koizumi N, Muto M, Yamada A, Watanabe H, , 2009. Prevalence of Leptospira spp. in the kidneys of wild boars and deer in Japan. J Vet Med Sci 71: 797799.[Crossref]
  28. Koizumi N, Uchida M, Makino T, Taguri T, Kuroki T, Muto M, Kato Y, Watanabe H, , 2009. Isolation and characterization of Leptospira spp. from raccoons in Japan. J Vet Med Sci 71: 425429.[Crossref]
  29. Masuzawa T, Okamoto Y, Une Y, Takeuchi T, Tsukagoshi K, Koizumi N, Kawabata H, Ohta S, Yoshikawa Y, , 2006. Leptospirosis in squirrels imported from United States to Japan. Emerg Infect Dis 12: 11531155.[Crossref]
  30. Matthias MA, Ricaldi JN, Cespedes M, Diaz MM, Galloway RL, Saito M, Steigerwalt AG, Patra KP, Ore CV, Gotuzzo E, Gilman RH, Levett PN, Vinetz JM, , 2008. Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. PLoS Negl Trop Dis 2: e213.[Crossref]
  31. Topacio TM, Gavino LG, Famatiga E, Suva M, , 1974. Leptospirosis in animals and man in the Philippines. VIII. Serological incidence in native dogs. Int J Zoonoses 1: 3242.
  32. Levett PN, , 2001. Leptospirosis. Clin Microbiol Rev 14: 296326.[Crossref]
  33. Levett PN, , 2007. Sequence-based typing of Leptospira: epidemiology in the genomic era. PLoS Negl Trop Dis 1: e120.[Crossref]
  34. Thaipadungpanit J, Wuthiekanun V, Chierakul W, Smythe LD, Petkanchanapong W, Limpaiboon R, Apiwatanaporn A, Slack AT, Suputtamongkol Y, White NJ, Feil EJ, Day NP, Peacock SJ, , 2007. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Negl Trop Dis 1: e56.[Crossref]
  35. Ahmed N, Devi SM, Valverde Mde L, Vijayachari P, Machang'u RS, Ellis WA, Hartskeerl RA, , 2006. Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Ann Clin Microbiol Antimicrob 5: 28.[Crossref]
  36. Kawabata H, Dancel LA, Villanueva SY, Yanagihara Y, Koizumi N, Watanabe H, , 2001. flaB-polymerase chain reaction (flaB-PCR) and its restriction fragment length polymorphism (RFLP) analysis are an efficient tool for detection and identification of Leptospira spp. Microbiol Immunol 45: 491496.[Crossref]
  37. Ribeiro RL, Machry L, Brazil JM, Ramos TM, Avelar KE, Pereira MM, , 2009. Technical improvement to prevent DNA degradation of Leptospira spp. in pulsed field gel electrophoresis. Lett Appl Microbiol 49: 289291.[Crossref]
  38. Slack AT, Symonds ML, Dohnt MF, Smythe LD, , 2006. Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol 6: 95.[Crossref]
  39. Slack AT, Khyairani-Bejo S, Symonds ML, Dohnt MF, Galloway RL, Steigerwalt AG, Bahaman AR, Craig S, Harrower BJ, Smythe LD, , 2009. Leptospira kmetyi sp. nov., isolated from an environmental source in Malaysia. Int J Syst Evol Microbiol 59: 705708.[Crossref]
  40. Slack At, Galloway RL, Symonds ML, Dohnt MF, Smythe LD, , 2009. Reclassfication of Leptospira meyeri serovar Perameles to Leptospira interrogans serovar Perameles through serological and molecular analysis: evidence of a need for changes to current procedures in Leptospira taxonomy. Int J Syst Evol Microbiol 59: 705708.[Crossref]
  41. Bharti AR, Nally JE, Ricaldi JN, Matthias MA, Diaz MM, Lovett MA, Levett PN, Gilman RH, Willig MR, Gotuzzo E, Vinetz JM, , 2003. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 3: 757771.[Crossref]
  42. Department of Health. Field Health Information System Annual Report, 2001. Manila: National Epidemiology Center of the Department of Health, Philippines.
  43. Carlos ER, Kundin WD, Watten RH, Tsai CC, Irving GS, Carlos ET, Directo AC, , 1971. Leptospirosis in the Philippines: feline studies. Am J Vet Res 32: 14551456.
  44. Kmety E, Dikken H, , 1993. Classification of the Species Leptospira interrogans and History of its Serovars. Groningen, The Netherlands: University Press.
  45. Geisen V, Stengel C, Brem S, Muller W, Greene C, Hartmann K, , 2007. Canine leptospirosis infections: clinical signs and outcome with different suspected Leptospira serogroups (42 cases). J Small Anim Pract 48: 324328.[Crossref]
  46. O'Keefe JS, Jenner JA, Sandifer NC, Antony A, Williamson NB, , 2002. A serosurvey for antibodies to Leptospira in dogs in the lower North Island of New Zealand. N Z Vet J 50: 2325.[Crossref]
  47. Thiermann AB, , 1981. The Norway rat as a selective chronic carrier of Leptospira icterohaemorrhagiae . J Wildl Dis 17: 3943.[Crossref]
  48. Haake DA, , 2006. Hamster model of leptospirosis. Curr Protoc Microbiol 12: Unit 12E 2.
  49. Ko AI, Goarant C, Picardeau M, , 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat Rev Microbiol 7: 736747.[Crossref]
  50. Yamamoto S, Harayama S, , 1995. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61: 11041109.
  51. Yamamoto S, Harayama S, , 1996. Phylogenetic analysis of Acetinobacter strains based on the nucleotide sequences of gyrB genes and on the amino acid sequences of their products. Int J Syst Bacteriol 46: 506511.[Crossref]
  52. Kasai H, Ezaki T, Harayama S, , 2000. Differentiation of phylogenetically related slowly growing Mycobacteria by their gyrB sequences. J Clin Microbiol 38: 301308.
  53. Galloway RL, Levett PN, , 2008. Evaluation of a modified pulsed-field gel electrophoresis approach for the identification of Leptospira serovars. Am J Trop Med Hyg 78: 628632.
  54. Herrmann JL, Baris C, Bellenger E, Perolat P, Baranton G, Saint Girons I, , 1991. Genome conservation in isolates of Leptospira interrogans . J Bacteriol 173: 75827588.[Crossref]
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  • Received : 30 Nov 2009
  • Accepted : 19 Jan 2010

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