Volume 83, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Acute febrile illnesses comprise the majority of the human disease burden in sub-Saharan Africa. We hypothesized that arboviruses comprised a considerable proportion of undiagnosed febrile illnesses in Guinea and sought to determine the frequency of arboviral disease in two hospitals there. Using a standard case definition, 47 suspected cases were detected in approximately 4 months. Immunoglobulin M antibody capture enzyme-linked immunosorbent assays and plaque-reduction neutralization assays revealed that 63% (30/47) of patients were infected with arboviruses, including 11 West Nile, 2 yellow fever, 1 dengue, 8 chikungunya, and 5 Tahyna infections. Except for yellow fever, these are the first reported cases of human disease from these viruses in Guinea and the first reported cases of symptomatic Tahyna infection in Africa. These results strongly suggest that arboviruses circulate and are common causes of disease in Guinea. Improving surveillance and laboratory capacity for arbovirus diagnoses will be integral to understanding the burden posed by these agents in the region.


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  1. Campbell JD, Sow SO, Levine MM, Kotloff KL, , 2004. The causes of hospital admission and death among children in Bamako, Mali. J Trop Pediatr 50: 158163.[Crossref] [Google Scholar]
  2. Breman JG, , 2001. The ears of the hippopotamus: manifestations, determinants, and estimates of the malaria burden. Am J Trop Med Hyg 64: 111. [Google Scholar]
  3. Krippner R, von Laer G, , 2002. First confirmed dengue-1 fever cases reported from Cameroon. J Travel Med 9: 273274.[Crossref] [Google Scholar]
  4. Ndip LM, Bouyer DH, Travassos Da Rosa APA, Titanji VPK, Tesh RB, Walker DH, , 2004. Acute spotted fever rickettsiosis among febrile patients, Cameroon. Emerg Infect Dis 10: 432437.[Crossref] [Google Scholar]
  5. Reyburn H, Mbatia R, Drakeley C, Carneiro I, Mwakasungula E, Mwerinde O, Saganda K, Shao J, Kitua A, Olomi R, Greenwood BM, Whitty CJ, , 2004. Overdiagnosis of malaria in patients with severe febrile illness in Tanzania: a prospective study. BMJ 329: 1212.[Crossref] [Google Scholar]
  6. Nsutebu EF, Martins P, Adiogo D, , 2003. Prevalence of typhoid fever in febrile patients with symptoms clinically compatible with typhoid fever in Cameroon. Trop Med Int Health 8: 575578.[Crossref] [Google Scholar]
  7. Anonymous, 2006. Yellow fever situation in Africa and South America, 2005. Wkly Epidemiol Rec 81: 317324. [Google Scholar]
  8. Anonymous, 2001. Yellow Fever. Geneva: World Health Organization, 13. [Google Scholar]
  9. Griffin DE, Knipe DM, Howley PM, , 2007. Alphaviruses. , eds. Fields Virology. Philadelphia, PA: Lippincott, Williams, and Wilkens, 10231067. [Google Scholar]
  10. Anonymous, 2003. Yellow fever vaccine: WHO position paper. Wkly Epidemiol Rec 78: 349359. [Google Scholar]
  11. Nathan N, Barry M, Van Herp M, Zeller H, , 2001. Shortage of vaccines during a yellow fever outbreak in Guinea. Lancet 358: 21292130.[Crossref] [Google Scholar]
  12. Butenko AM, , 1996. Arbovirus circulation in the Republic of Guinea. Med Parazitol (Mosk): 4045. [Google Scholar]
  13. Ivanov AP, Ivanova OE, Lomonosov NN, Pozdnyakov SV, Konstantinov OK, Bah MA, , 1992. Serological investigations of Chikungunya virus in the Republic of Guinea. Ann Soc Belg Med Trop 72: 7374. [Google Scholar]
  14. Powers AM, Logue CH, , 2007. Changing patterns of chikungunya virus: re-emergence of a zoonotic arbovirus. J Gen Virol 88: 23632377.[Crossref] [Google Scholar]
  15. Konstantinov OK, , 1990. Ticks of the Ixodidae family as reservoir of arboviruses in the Republic of Guinea. II. Arboviruses. Rev Elev Med Vet Pays Trop 43: 1522. [Google Scholar]
  16. Tkachenko EA, Butenko AM, Leshchinskaya EV, Bouaro I, Ivanov AP, Dzagurova TK, Sochinski VA, , 1984. Serological investigation of population from the People's Revolutionary Republic of Guinea for antibodies to viral haemorrhagic fevers. Proceedings of the 11th International Congress for Tropical Medicine and Malaria, Calgary, Canada September 16–22, 1984. [Google Scholar]
  17. Boiro MY, Lama N, Barry M, Diallo R, Morillon M, , 1999. Cholera in Guinea: the 1994–1995 epidemic. Med Trop (Mars) 59: 303306. [Google Scholar]
  18. Boiro I, Konstaninov OK, Numerov AD, , 1987. Isolation of Rift Valley fever virus from bats in the Republic of Guinea. Bull Soc Pathol Exot Filiales 80: 6267. [Google Scholar]
  19. Boiro I, Lomonossov NN, Sotsinski VA, Constantinov OK, Tkachenko EA, Inapogui AP, Balde C, , 1987. Clinico-epidemiologic and laboratory research on hemorrhagic fevers in Guinea. Bull Soc Pathol Exot Filiales 80: 607612. [Google Scholar]
  20. Boiro I, Lomonossov NN, Alexin AF, Bah A, Balde C, , 1986. Isolation of a new orbivirus Kindia (Palyam group) from ticks Amblyomma variegatum in the Republic of Guinea. Bull Soc Pathol Exot Filiales 79: 187190. [Google Scholar]
  21. Boiro I, Lomonossov NN, Fidarov FM, Murzine SV, Linev NB, Camara LB, Bah A, , 1985. Isolation of M'Poko virus (Turlock group, Bunyaviridae) from Culex cinereus mosquitoes in the Republic of Guinea. Bull Soc Pathol Exot Filiales 78: 452455. [Google Scholar]
  22. Konstantinov OK, Buaro I, Chunina LM, Balde S, Murzin SV, , 1988. Ticks of the family Ixodidae in Guinea and the isolation of arboviruses. 1. Species composition of ticks. Med Parazitol (Mosk) 2: 6468. [Google Scholar]
  23. Bausch DG, Demby AH, Coulibaly M, Kanu J, Goba A, Bah A, Conde N, Wurtzel HL, Cavallaro KF, Lloyd E, Baldet FB, Csse SD, Fofona D, Savane IK, Tolno RT, Mahy B, Wagoner KD, Ksiazek TG, Peters CJ, Rollin PE, , 2001. Lassa fever in Guinea: I. Epidemiology of human disease and clinical observations. Vector Borne Zoonotic Dis 1: 269281.[Crossref] [Google Scholar]
  24. Martin DA, Biggerstaff BJ, Allen B, Johnson AJ, Lanciotti RS, Roehrig JT, , 2002. Use of immunoglobulin m cross-reactions in differential diagnosis of human flaviviral encephalitis infections in the United States. Clin Diagn Lab Immunol 9: 544549. [Google Scholar]
  25. Martin DA, Muth DA, Brown T, Johnson AJ, Karabatsos N, Roehrig JT, , 2000. Standardization of immunoglobulin M capture enzyme-linked immunosorbent assays for routine diagnosis of arboviral infections. J Clin Microbiol 38: 18231826. [Google Scholar]
  26. Beaty B, Calisher C, Shope R, Lennette E, Lennette D, Lennette E, , 1995. Arboviruses. , eds. Diagnostic Procedures for Viral, Rickettsial, and Chlamydial Infections. Washington, DC: American Public Health Association, 189212. [Google Scholar]
  27. World Health Organization, 2007. Guidelines for Plaque Reduction Neutralizing Testing of Human Antibodies to Dengue Viruses. Geneva: World Health Organization. [Google Scholar]
  28. Lindsey HS, Calisher CH, Mathews JH, , 1976. Serum dilution neutralization test for California group virus identification and serology. J Clin Microbiol 4: 503510. [Google Scholar]
  29. Calisher CH, Karabatsos N, Dalrymple JM, Shope RE, Porterfield JS, Westaway EG, Brandt WE, , 1989. Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. J Gen Virol 70: 3743.[Crossref] [Google Scholar]
  30. Anonymous, 2005. The yellow fever situation in Africa and South America in 2004. Wkly Epidemiol Rec 80: 249256. [Google Scholar]
  31. Anonymous, 2009. GAaR, ed. Outbreaks of Yellow Fever. Geneva, Switzerland: World Health Organization. [Google Scholar]
  32. Konstantinov OK, Diallo SM, Inapogi AP, Ba A, Kamara SK, , 2006. The mammals of Guinea as reservoirs and carriers of arboviruses. Med Parazitol (Mosk): 3439. [Google Scholar]
  33. Paquet C, Quatresous I, Solet J-L, Sissoko D, Renault P, Pierre V, Cordel H, Lassalle C, Thiria J, Zeller H, Schuffnecker I, , 2006. Épidémiologie de l'infection par le virus Chikungunya à l'Ile de la Réunion: point de la situation au 8 janvier 2006. Bulletin épidémiologique hebdomidaire, Special Issue, 31 January. [Google Scholar]
  34. Tsai TF, Monath TP, , 1997. Alphaviruses. J Clin Virol 12171255. [Google Scholar]
  35. Thonnon J, Spiegel A, Diallo M, Diallo A, Fontenille D, , 1999. Chikungunya virus outbreak in Senegal in 1996 and 1997. Bull Soc Pathol Exot 92: 7982. [Google Scholar]
  36. Weaver SC, Tesh RB, Shope RE, Guerrant RL, Walker DH, Weller PF, , 2006. Alphavirus infections. , eds. Essentials of Tropical Infectious Disease. New York: Churchill Livingstone, 587590. [Google Scholar]
  37. Kokernot RH, Szlamp EL, Levitt J, McIntosh BM, , 1965. Survey for antibodies against arthropod-borne viruses in the sera of indigenous residents of the Caprivi Strip and Bechuanaland Protectorate. Trans R Soc Trop Med Hyg 59: 553562.[Crossref] [Google Scholar]
  38. Kokernot RH, Smithburn KC, Gandara AF, McIntosh BM, Heymann CS, , 1960. Neutralization tests with sera from individuals residing in Mozambique against specific viruses isolated in Africa, transmitted by arthropods. An Inst Med Trop (Lisb) 17: 201230. [Google Scholar]
  39. van den Bosch C, Lloyd G, , 2000. Chikungunya fever as a risk factor for endemic Burkitt's lymphoma in Malawi. Trans R Soc Trop Med Hyg 94: 704705.[Crossref] [Google Scholar]
  40. Rodhain F, Carteron B, Laroche R, Hannoun C, , 1987. Human arbovirus infections in Burundi: results of a seroepidemiologic survey, 1980–1982. Bull Soc Pathol Exot Filiales 80: 155161. [Google Scholar]
  41. Adekolu-John EO, Fagbami AH, , 1983. Arthropod-borne virus antibodies in sera of residents of Kainji Lake Basin, Nigeria 1980. Trans R Soc Trop Med Hyg 77: 149151.[Crossref] [Google Scholar]
  42. Saluzzo JF, Cornet M, Castagnet P, Rey C, Digoutte JP, , 1986. Isolation of dengue 2 and dengue 4 viruses from patients in Senegal. Trans R Soc Trop Med Hyg 80: 5.[Crossref] [Google Scholar]
  43. Traore-Lamizana M, Zeller HG, Monlun E, Mondo M, Hervy JP, Adam F, Digoutte JP, , 1994. Dengue 2 outbreak in southeastern Senegal during 1990: virus isolations from mosquitoes (Diptera: Culicidae). J Med Entomol 31: 623627.[Crossref] [Google Scholar]
  44. Durand JP, Vallee L, de Pina JJ, Tolou H, , 2000. Isolation of a dengue type 1 virus from a soldier in West Africa (Cote d'Ivoire). Emerg Infect Dis 6: 8384.[Crossref] [Google Scholar]
  45. Roche C, Cordellier R, Hervy JP, , 1983. Isolement de 96 souches de virus dengue 2 a partir de moustiques captures en Cote d'Ivoire et Haute-Volta. Ann Inst Pasteur Virol 134E: 233244.[Crossref] [Google Scholar]
  46. Cordellier R, Bouchite B, Roche J, Diaco B, Akoliba P, , 1983. Circulation selvatique du virus dengue 2 en 1980, dans les savanes sub-soudaniennes de Cote d'Ivoire. Ent Med et Parasitol 21: 165179. [Google Scholar]
  47. Diallo M, Thonnon J, Traore-Lamizana M, Fontenille D, , 1999. Vectors of Chikungunya virus in Senegal: current data and transmission cycles. Am J Trop Med Hyg 60: 281286. [Google Scholar]
  48. Burke DS, Monath TP., Knipe DM, Howley PM, Flaviviruses. , eds.Field's Virology. Philadelphia, PA: Lippincott, Williams, and Wilkens. 10431125. [Google Scholar]
  49. Gubler DJ, Sather GE, Kuno G, Cabral JR, , 1986. Dengue 3 virus transmission in Africa. Am J Trop Med Hyg 35: 12801284. [Google Scholar]
  50. Franco L, DiCaro A, Carletti F, Vapalahti O, Renaudat C, Zeller H, Tenorio A, , 2010. Recent expansion of dengue virus serotype 3 in West Africa. Euro Surveill 15: 2. [Google Scholar]
  51. Ninove L, Parola P, Baronti C, De Lamballerie X, Gautret P, Doudier B, Charrel RN, , 2009. Dengue virus type 3 infection in traveler returning from west Africa. Emerg Infect Dis 15: 18711872.[Crossref] [Google Scholar]
  52. Vazeille-Falcoz M, Failloux AB, Mousson L, Elissa N, Rodhain F, , 1999. Oral receptivity of Aedes aegypti formosus from Franceville (Gabon, central Africa) for type 2 dengue virus. Bull Soc Pathol Exot 92: 341342. [Google Scholar]
  53. Gubler DJ, , 2002. The global emergence/resurgence of arboviral diseases as public health problems. Arch Med Res 33: 330342.[Crossref] [Google Scholar]
  54. Watts DM, Porter KR, Putvatana P, Vasquez B, Calampa C, Hayes CG, Halstead SB, , 1999. Failure of secondary infection with American genotype dengue 2 to cause dengue haemorrhagic fever. Lancet 354: 14311434.[Crossref] [Google Scholar]
  55. Rico-Hesse R, , 1990. Molecular evolution and distribution of dengue viruses type 1 and 2 in nature. Virology 174: 479493.[Crossref] [Google Scholar]
  56. Renaudet J, Jan C, Ridet J, Adam C, Robin Y, , 1978. A serological survey of arboviruses in the human population of Senegal. Bull Soc Pathol Exot Filiales 71: 131140. [Google Scholar]
  57. Chevalier V, Lancelot R, Diaite A, Mondet B, Sall B, De Lamballerie X, , 2006. Serological assessment of West Nile fever virus activity in the pastoral system of Ferlo, Senegal. Ann N Y Acad Sci 1081: 216225.[Crossref] [Google Scholar]
  58. Cabre O, Grandadam M, Marie JL, Gravier P, Prange A, Santinelli Y, Rous V, Bourry O, Durand JP, Tolou H, Davoust B, , 2006. West Nile Virus in horses, sub-Saharan Africa. Emerg Infect Dis 12: 19581960.[Crossref] [Google Scholar]
  59. Estival JL, Skowron F, Dupin M, Combemale P, , 2001. Primary infection with West-Nile virus. Ann Dermatol Venereol 128: 656658. [Google Scholar]
  60. Traore-Lamizana M, Zeller HG, Mondo M, Hervy JP, Adam F, Digoutte JP, , 1994. Isolations of West Nile and Bagaza viruses from mosquitoes (Diptera: Culicidae) in central Senegal (Ferlo). J Med Entomol 31: 934938.[Crossref] [Google Scholar]
  61. Kuniholm MH, Wolfe ND, Huang CY, Mpoudi-Ngole E, Tamoufe U, Burke DS, Gubler DJ, , 2006. Seroprevalence and distribution of flaviviridae, togaviridae, and bunyaviridae arboviral infections in rural Cameroonian adults. Am J Trop Med Hyg 74: 10781083. [Google Scholar]
  62. Chastel C, Bach-Hamba D, Launay H, Le Lay G, Hellal H, Beaucournu JC, , 1983. Arbovirus infections in Tunisia: new serological survey of small wild mammals. Bull Soc Pathol Exot Filiales 76: 2133. [Google Scholar]

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  • Received : 17 Nov 2009
  • Accepted : 14 Apr 2010
  • Published online : 05 Aug 2010

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