Volume 82, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Four major malaria-causing spp. and lymphatic filariasis-causing are co-endemic in many tropical and sub-tropical regions. Among molecular diagnostic assays, multiplex polymerase chain reaction (PCR)–based assays for the simultaneous detection of DNAs from these parasite species are currently available only for and or and . Using a post-PCR oligonucleotide ligation detection reaction–fluorescent microsphere assay (LDR-FMA), we developed a multiplex assay that has the capability to simultaneously detect all four spp. and infections in blood samples. Compared with microfilarial positivity in the blood, the LDR-FMA assay is highly concordant (91%), sensitive (86%), and specific (94%), and has high reproducibility for spp. (85–93%) and (90%) diagnoses. The development of this assay for the simultaneous diagnosis of multiple parasitic infections enables efficient screening of large numbers of human blood and mosquito samples from co-endemic areas.


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  1. Hotez PJ, Remme JH, Buss P, Alleyne G, Morel C, Breman JG, , 2004. Combating tropical infectious diseases: report of the Disease Control Priorities in Developing Countries Project. Clin Infect Dis 38: 871878.[Crossref] [Google Scholar]
  2. Michael E, Bundy DA, , 1997. Global mapping of lymphatic filariasis. Parasitol Today 13: 472476.[Crossref] [Google Scholar]
  3. Muturi EJ, Jacob BG, Kim CH, Mbogo CM, Novak RJ, , 2008. Are coinfections of malaria and filariasis of any epidemiological significance? Parasitol Res 102: 175181.[Crossref] [Google Scholar]
  4. Reeder JC, , 2003. Health research in Papua New Guinea. Trends Parasitol 19: 241245.[Crossref] [Google Scholar]
  5. Chadee DD, Rawlins SC, Tiwari TS, , 2003. Short communication: concomitant malaria and filariasis infections in Georgetown, Guyana. Trop Med Int Health 8: 140143.[Crossref] [Google Scholar]
  6. Ghosh SK, Yadav RS, , 1995. Naturally acquired concomitant infections of bancroftian filariasis and human plasmodia in Orissa. Indian J Malariol 32: 3236. [Google Scholar]
  7. Muturi EJ, Mbogo CM, Mwangangi JM, Ng'ang'a ZW, Kabiru EW, Mwandawiro C, Beier JC, , 2006. Concomitant infections of Plasmodium falciparum and Wuchereria bancrofti on the Kenyan coast. Filaria J 5: 8.[Crossref] [Google Scholar]
  8. Burkot TR, Molineaux L, Graves PM, Paru R, Battistutta D, Dagoro H, Barnes A, Wirtz RA, Garner P, , 1990. The prevalence of naturally acquired multiple infections of Wuchereria bancrofti and human malarias in anophelines. Parasitology 100: 369375.[Crossref] [Google Scholar]
  9. Campbell CC, , 2008. Halting the toll of malaria in Africa. Am J Trop Med Hyg 78: 851853. [Google Scholar]
  10. Weil GJ, Ramzy RM, , 2007. Diagnostic tools for filariasis elimination programs. Trends Parasitol 23: 7882.[Crossref] [Google Scholar]
  11. Melrose WD, Durrheim DD, Burgess GW, , 2004. Update on immunological tests for lymphatic filariasis. Trends Parasitol 20: 255257.[Crossref] [Google Scholar]
  12. Nuchprayoon S, Porksakorn C, Junpee A, Sanprasert V, Poovorawan Y, , 2003. Comparative assessment of an Og4C3 ELISA and an ICT filariasis test: a study of Myanmar migrants in Thailand. Asian Pac J Allergy Immunol 21: 253257. [Google Scholar]
  13. Pani SP, Hoti SL, Vanamail P, Das LK, , 2004. Comparison of an immunochromatographic card test with night blood smear examination for detection of Wuchereria bancrofti microfilaria carriers. Natl Med J India 17: 304306. [Google Scholar]
  14. Berry A, Benoit-Vical F, Fabre R, Cassaing S, Magnaval JF, , 2008. PCR-based methods to the diagnosis of imported malaria. Parasite 15: 484488.[Crossref] [Google Scholar]
  15. Erdman LK, Kain KC, , 2008. Molecular diagnostic and surveillance tools for global malaria control. Travel Med Infect Dis 6: 8299.[Crossref] [Google Scholar]
  16. Bisht R, Hoti SL, Thangadurai R, Das PK, , 2006. Isolation of Wuchereria bancrofti microfilariae from archived stained blood slides for use in genetic studies and amplification of parasite and endosymbiont genes. Acta Trop 99: 15.[Crossref] [Google Scholar]
  17. Kanjanavas P, Tan-ariya P, Khawsak P, Pakpitcharoen A, Phantana S, Chansiri K, , 2005. Detection of lymphatic Wuchereria bancrofti in carriers and long-term storage blood samples using semi-nested PCR. Mol Cell Probes 19: 169172.[Crossref] [Google Scholar]
  18. Rao RU, Atkinson LJ, Ramzy RM, Helmy H, Farid HA, Bockarie MJ, Susapu M, Laney SJ, Williams SA, Weil GJ, , 2006. A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes. Am J Trop Med Hyg 74: 826832. [Google Scholar]
  19. Zhong M, McCarthy J, Bierwert L, Lizotte-Waniewski M, Chanteau S, Nutman TB, Ottesen EA, Williams SA, , 1996. A polymerase chain reaction assay for detection of the parasite Wuchereria bancrofti in human blood samples. Am J Trop Med Hyg 54: 357363. [Google Scholar]
  20. Chansiri K, Kwoasak P, Tananyutthawongese C, Sukhumsirichart W, Sarataphan N, Phantana S, , 2001. Detection of Plasmodium falciparum and Wuchereria bancrofti infected blood samples using multiplex PCR. Mol Cell Probes 15: 201207.[Crossref] [Google Scholar]
  21. Rao RU, Huang Y, Bockarie MJ, Susapu M, Laney SJ, Weil GJ, , 2009. A qPCR-based multiplex assay for the detection of Wuchereria bancrofti, Plasmodium falciparum and Plasmodium vivax DNA. Trans R Soc Trop Med Hyg 103: 365370.[Crossref] [Google Scholar]
  22. Muller I, Bockarie M, Alpers M, Smith T, , 2003. The epidemiology of malaria in Papua New Guinea. Trends Parasitol 19: 253259.[Crossref] [Google Scholar]
  23. Mehlotra RK, Kasehagen LJ, Baisor M, Lorry K, Kazura JW, Bockarie MJ, Zimmerman PA, , 2002. Malaria infections are randomly distributed in diverse holoendemic areas of Papua New Guinea. Am J Trop Med Hyg 67: 555562. [Google Scholar]
  24. Mehlotra RK, Lorry K, Kastens W, Miller SM, Alpers MP, Bockarie M, Kazura JW, Zimmerman PA, , 2000. Random distribution of mixed species malaria infections in Papua New Guinea. Am J Trop Med Hyg 62: 225231. [Google Scholar]
  25. McNamara DT, Kasehagen LJ, Grimberg BT, Cole-Tobian J, Collins WE, Zimmerman PA, , 2006. Diagnosing infection levels of four human malaria parasite species by a polymerase chain reaction/ligase detection reaction fluorescent microsphere-based assay. Am J Trop Med Hyg 74: 413421. [Google Scholar]
  26. Kasehagen LJ, Mueller I, McNamara DT, Bockarie MJ, Kiniboro B, Rare L, Lorry K, Kastens W, Reeder JC, Kazura JW, Zimmerman PA, , 2006. Changing patterns of Plasmodium blood-stage infections in the Wosera region of Papua New Guinea monitored by light microscopy and high throughput PCR diagnosis. Am J Trop Med Hyg 75: 588596. [Google Scholar]
  27. Mueller I, Widmer S, Michel D, Maraga S, McNamara DT, Kiniboro B, Sie A, Smith TA, Zimmerman PA, , 2009. High sensitivity detection of Plasmodium species reveals positive correlations between infections of different species, shifts in age distribution and reduced local variation in Papua New Guinea. Malar J 8: 41.[Crossref] [Google Scholar]
  28. Bockarie MJ, Kazura JW, , 2003. Lymphatic filariasis in Papua New Guinea: prospects for elimination. Med Microbiol Immunol (Berl) 192: 914. [Google Scholar]
  29. Bockarie MJ, Tisch DJ, Kastens W, Alexander ND, Dimber Z, Bockarie F, Ibam E, Alpers MP, Kazura JW, , 2002. Mass treatment to eliminate filariasis in Papua New Guinea. N Engl J Med 347: 18411848.[Crossref] [Google Scholar]
  30. Tisch DJ, Bockarie MJ, Dimber Z, Kiniboro B, Tarongka N, Hazlett FE, Kastens W, Alpers MP, Kazura JW, , 2008.Mass drug administration trial to eliminate lymphatic filariasis in Papua New Guinea: changes in microfilaremia, filarial antigen, and Bm14 antibody after cessation. Am J Trop Med Hyg 78: 289293. [Google Scholar]
  31. Tisch DJ, Hazlett FE, Kastens W, Alpers MP, Bockarie MJ, Kazura JW, , 2001. Ecologic and biologic determinants of filarial antigenemia in bancroftian filariasis in Papua New Guinea. J Infect Dis 184: 898904.[Crossref] [Google Scholar]
  32. Bockarie MJ, Alexander ND, Hyun P, Dimber Z, Bockarie F, Ibam E, Alpers MP, Kazura JW, , 1998. Randomised community-based trial of annual single-dose diethylcarbamazine with or without ivermectin against Wuchereria bancrofti infection in human beings and mosquitoes. Lancet 351: 162168.[Crossref] [Google Scholar]
  33. Ngwira BM, Jabu CH, Kanyongoloka H, Mponda M, Crampin AC, Branson K, Alexander ND, Fine PE, , 2002. Lymphatic filariasis in the Karonga district of northern Malawi: a prevalence survey. Ann Trop Med Parasitol 96: 137144.[Crossref] [Google Scholar]
  34. Cox-Singh J, Davis TM, Lee KS, Shamsul SS, Matusop A, Ratnam S, Rahman HA, Conway DJ, Singh B, , 2008. Plasmodium knowlesi malaria in humans is widely distributed and potentially life threatening. Clin Infect Dis 46: 165171.[Crossref] [Google Scholar]

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  • Received : 05 Nov 2009
  • Accepted : 11 Jan 2010
  • Published online : 04 Jun 2010

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