Volume 83, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The Grand Cayman population of is highly resistant to DDT and pyrethroid insecticides. Glutathione transferase, cytochrome P450, and esterase levels were increased in the Grand Cayman population relative to a susceptible laboratory strain, but synergist studies did not implicate elevated insecticide detoxification as a major cause of resistance. The role of target site resistance was therefore investigated. Two substitutions in the voltage-gated sodium channel were identified, V1016I in domain II, segment 6 (IIS6) (allele frequency = 0.79) and F1534C in IIIS6 (allele frequency = 0.68). The role of the F1534C mutation in conferring resistance to insecticides has not been previously established and so a tetraplex polymerase chain reaction assay was designed and used to genotype mosquitoes that had been exposed to insecticides. The F1534C mutation was strongly correlated with resistance to DDT and permethrin.


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  1. Gubler DJ, , 2002. The global emergence/resurgence of arboviral diseases as public health problems. Arch Med Res 33: 330342.[Crossref]
  2. Brown AW, , 1986. Insecticide resistance in mosquitoes: a pragmatic review. J Am Mosq Control Assoc 2: 123140.
  3. Mekuria Y, Gwinn TA, Williams DC, Tidwell MA, , 1991. Insecticide susceptibility of Aedes aegypti from Santo-Domingo, Dominican-Republic. J Am Mosq Control Assoc 7: 6972.
  4. Rawlins SC, Wan JO, , 1995. Resistance in some Caribbean populations of Aedes aegypti to several insecticides. J Am Mosq Control Assoc 11: 5965.
  5. Rawlins SC, , 1998. Spatial distribution of insecticide resistance in Caribbean populations of Aedes aegypti and its significance. Rev Panam Salud Publica 4: 243251.[Crossref]
  6. Wirth MC, Georghiou GP, , 1999. Selection and characterization of temephos resistance in a population of Aedes aegypti from Tortola, British Virgin Islands. J Am Mosq Control Assoc 15: 315320.
  7. Rodriguez MM, Bisset J, De Fernandez DM, Lauzan L, Soca A, , 2001. Detection of insecticide resistance in Aedes aegypti (Diptera: Culicidae) from Cuba and Venezuela. J Med Entomol 38: 623628.[Crossref]
  8. Hemingway J, Boddington RG, Harris J, Dunbar SJ, , 1989. Mechanisms of insecticide resistance in Aedes aegypti (L.) (Diptera, Culicidae) from Puerto-Rico. Bull Entomol Res 79: 123130.[Crossref]
  9. Brengues C, Hawkes NJ, Chandre F, McCarroll L, Duchon S, Guillet P, Manguin S, Morgan JC, Hemingway J, , 2003. Pyrethroid and DDT cross-resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene. Med Vet Entomol 17: 8794.[Crossref]
  10. Vaughan A, Chadee DD, Ffrench-Constant R, , 1998. Biochemical monitoring of organophosphorus and carbamate insecticide resistance in Aedes aegypti mosquitoes from Trinidad. Med Vet Entomol 12: 318321.[Crossref]
  11. Strode C, Wondji CS, David JP, Hawkes NJ, Lumjuan N, Nelson DR, Drane DR, Karunaratne S, Hemingway J, Black WC, Ranson H, , 2008. Genomic analysis of detoxification genes in the mosquito Aedes aegypti . Insect Biochem Mol Biol 38: 113123.[Crossref]
  12. Marcombe S, Poupardin R, Darriet F, Reynaud S, Bonnet J, Strode C, Brengues C, Yebakima A, Ranson H, Corbel V, David JP, , 2009. Exploring the molecular basis of insecticide resistance in the dengue vector Aedes aegypti: a case study in Martinique Island (French West Indies). BMC Genomics 10: 494.[Crossref]
  13. Saavedra-Rodriguez K, Urdaneta-Marquez L, Rajatileka S, Moulton M, Flores AE, Fernandez-Salas I, Bisset J, Rodriguez M, McCall PJ, Donnelly MJ, Ranson H, Hemingway J, Black WC, , 2007. A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti . Insect Mol Biol 16: 785798.[Crossref]
  14. Chang C, Shen WK, Wang TT, Lin YH, Hsu EL, Dai SM, , 2009. A novel amino acid substitution in a voltage-gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti . Insect Biochem Mol Biol 39: 272278.[Crossref]
  15. Coto MM, Lazcano JA, De Fernandez DM, Soca A, , 2000. Malathion resistance in Aedes aegypti and Culex quinquefasciatus after its use in Aedes aegypti control programs. J Am Mosq Control Assoc 16: 324330.
  16. WHO, 2005. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. WHO/CDS/WHOPES/GCDPP/2005.13.
  17. Penilla RP, Rodriguez AD, Hemingway J, Torres JL, Arredondo-Jimenez JI, Rodriguez MH, , 1998. Resistance management strategies in malaria vector mosquito control. Baseline data for a large-scale field trial against Anopheles albimanus in Mexico. Med Vet Entomol 12: 217233.[Crossref]
  18. Livak KJ, , 1984. Organization and mapping of a sequence on the drosophila-melanogaster X-chromosome and Y-chromosome that is transcribed during spermatogenesis. Genetics 107: 611634.
  19. Rajatileka S, Black WC IV, Saavedra-Rodriguez K, Trongtokit Y, Apiwathnasorn C, McCall PJ, Ranson H, , 2008. Development and application of a simple colorimetric assay reveals widespread distribution of sodium channel mutations in Thai populations of Aedes aegypti . Acta Trop 108: 5457.[Crossref]
  20. Rousset F, , 2008. GENEPOP'007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8: 103106.[Crossref]
  21. Williamson MS, Martinez-Torres D, Hick CA, Devonshire AL, , 1996. Identification of mutations in the housefly para-type sodium channel gene associated with knockdown resistance (kdr) to pyrethroid insecticides. Mol Gen Genet 252: 5160.[Crossref]
  22. Martinez-Torres D, Chandre F, Williamson MS, Darriet F, Berge JB, Devonshire AL, Guillet P, Pasteur N, Pauron D, , 1998. Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s. Insect Mol Biol 7: 179184.[Crossref]
  23. Brown AW, Pal R, , 1971. Insecticide resistance in arthropods. Public Health Pap 38: 1491.
  24. Khot AC, Bingham G, Field LM, Moores GD, , 2008. A novel assay reveals the blockade of esterases by piperonyl butoxide. Pest Manag Sci 64: 11391142.[Crossref]
  25. Sun YP, Johnson ER, , 1960. Synergistic and antagonistic actions of insecticide-synergist combinations and their mode of action. J Agric Food Chem 8: 261266.[Crossref]
  26. Lumjuan N, McCarroll L, Prapanthadara LA, Hemingway J, Ranson H, , 2005. Elevated activity of an Epsilon class glutathione transferase confers DDT resistance in the dengue vector, Aedes aegypti . Insect Biochem Mol Biol 35: 861871.[Crossref]
  27. Ponce García G, Flores AE, Fernandez-Salas I, Saavedra-Rodriguez K, Reyes-Solis G, Lozano-Fuentes S, Bond JG, Casas-Martínez M, Ramsay JM, García-Rejón J, Domínguez-Galera M, Ranson H, Hemingway J, Eisen L, Black WC IV, , 2009. Recent rapid rise of a permethrin knock down resistance allele in Aedes aegypti in Mexico. PLoS Negl Trop Dis 3: e531.[Crossref]
  28. O'Reilly AO, Khambay BP, Williamson MS, Field LM, Wallace BA, Davies TG, , 2006. Modelling insecticide-binding sites in the voltage-gated sodium channel. Biochem J 396: 255263.[Crossref]
  29. He HQ, Chen AC, Davey RB, Ivie GW, George JE, , 1999. Identification of a point mutation in the para-type sodium channel gene from a pyrethroid-resistant cattle tick. Biochem Biophys Res Commun 261: 558561.[Crossref]
  30. Tsagkarakou A, Van Leeuwen T, Khajehali J, Ilias A, Grispou M, Williamson MS, Tirry L, Vontas J, , 2009. Identification of pyrethroid resistance associated mutations in the para sodium channel of the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae). Insect Mol Biol 18: 583593.[Crossref]
  31. Du Y, Lee JE, Nomura Y, Zhang TX, Zhorov BS, Dong K, , 2009. Identification of a cluster of residues in transmembrane segment 6 of domain III of the cockroach sodium channel essential for the action of pyrethroid insecticides. Biochem J 419: 377385.[Crossref]

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  • Received : 15 Oct 2009
  • Accepted : 19 Apr 2010

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