1921
Volume 83, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The aim of the present preliminary study was to investigate the potential of measurement of IFN-γ secretion by T cells into blood plasma using QuantiFERON assay with leishmanial antigens to determine the presence of infection. Blood samples from cured visceral ( = 18), and cutaneous ( = 20) leishmaniasis cases, and 20 healthy controls were tested. The IFN-γ responses to H2B and H2B antigens were detected from the majority of treated old visceral leishmaniasis cases, but not from controls. Future studies using larger groups will be required to establish the true potential of the assay for epidemiological screening of leishmaniasis.

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2010-10-05
2017-09-24
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References

  1. Sadeghian G, Momeni A, Siadat AH, Usefi P, , 2006. Evaluation of leishmanin skin test and its relationship with the clinical form and duration of cutaneous leishmaniasis. Dermatol Online J 12: 3.
  2. Bern C, Amann J, Haque R, Chowdhury R, Ali M, Kurkjian KM, Vaz L, Wagatsuma Y, Breiman RF, Secor WE, Maguire JH, , 2006. Loss of leishmanin skin test antigen sensitivity and potency in a longitudinal study of visceral leishmaniasis in Bangladesh. Am J Trop Med Hyg 75: 744748.
  3. Herwaldt BL, , 1999. Leishmaniasis. Lancet 354: 11911199.[Crossref]
  4. Gidwani K, Rai M, Chakravarty J, Boelaert M, Sundar S, , 2009. Evaluation of leishmanin skin test in Indian visceral leishmaniasis. Am J Trop Med Hyg 80: 566567.
  5. Dheda K, Smit RZ, Badri M, Pai M, , 2009. T-cell interferon-gamma release assays for the rapid immunodiagnosis of tuberculosis: clinical utility in high-burden vs. low-burden settings. Curr Opin Pulm Med 15: 188200.[Crossref]
  6. Katial RK, Hershey J, Purohit-Seth T, Belisle JT, Brennan PJ, Spencer JS, Engler RJ, , 2001. Cell-mediated immune response to tuberculosis antigens: comparison of skin testing and measurement of in vitro gamma interferon production in whole-blood culture. Clin Diagn Lab Immunol 8: 339345.
  7. Mazurek GH, LoBue PA, Daley CL, Bernardo J, Lardizabal AA, Bishai WR, Iademarco MF, Rothel JS, , 2001. Comparison of a whole-blood interferon gamma assay with tuberculin skin testing for detecting latent Mycobacterium tuberculosis infection. JAMA 286: 17401747.[Crossref]
  8. Mol CJ, Koethe SM, , 2006. QuantiFERON TB GOLD: an innovation in tuberculosis screening. AAOHN J 54: 245247.[Crossref]
  9. Ehlers S, Smith KA, , 1991. Differentiation of T cell lymphokine gene expression: the in vitro acquisition of T cell memory. J Exp Med 173: 2536.[Crossref]
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  • Received : 09 Oct 2009
  • Accepted : 03 May 2010

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