1921
Volume 82, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

is the causative agent of visceral leishmaniasis in Brazil. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of kinetoplast DNA (kDNA) minicircles was used to evaluate genetic profiles of 48 strains from dog and human parasite cultures, fresh collected dog bone marrow aspirates, and from infected sand flies. Results revealed that heterogeneity in kDNA minicircles depends mostly on the source of the samples, with cultured parasites showing a high degree of homogeneity.

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References

  1. Health Ministry of Brazil, 2006. Visceral Leishmaniasis Surveillance and Control Manual. Brasília. Available at: http://portal.saude.gov.br/portal/arquivos/pdf/manual_leish_visceral2006.pdf.
  2. Tintaya KW, Ying X, Dedet JP, Rijal S, De Bolle X, Dujardin JC, , 2004. Antigen genes for molecular epidemiology of leishmaniasis: polymorphism of cysteine proteinase B and surface metalloprotease glycoprotein 63 in the Leishmania donovani complex. J Infect Dis 189: 10351043.[Crossref]
  3. Mauricio IL, Stothard JR, Miles MA, , 2004. Leishmania donovani complex: genotyping with the ribosomal internal transcribed spacer and the miniexon. Parasitol 128: 263267.[Crossref]
  4. Ochsenreither S, Kuhls K, Schaar M, Presber W, Schönian G, , 2006. Multilocus microsatellite typing as a new tool for discrimination of Leishmania infantum MON-1 strains. J Clin Microbiol 44: 495503.[Crossref]
  5. Cortes S, Mauricio I, Almeida A, Cristovao JM, Pratlong F, Dedet JP, Campino L, , 2006. Application of kDNA as a molecular marker to analyse Leishmania infantum diversity in Portugal. Parasitol Int 55: 277283.[Crossref]
  6. Laurent T, Rijal S, Yardley V, Croft S, De Doncker S, Decuypere S, Khanal B, Singh R, Schönian G, Kuhls K, Chappuis F, Dujardin J, , 2007. Epidemiological dynamics of antimonial resistance in Leishmania donovani: genotyping reveals a polyclonal population structure among naturally-resistant clinical isolates from Nepal. Inf. Gen. Evol. 7: 206212.[Crossref]
  7. Liu B, Liu Y, Motyka SA, Agbo EE, Englund PT, , 2005. Fellowship of the rings: the replication of kinetoplast DNA Trend. Parasitol. 21: 363369.
  8. Aransay AM, Scoulica E, Tselentis Y, , 2000. Detection and identification of Leishmania DNA within naturally infected sand flies by semi-nested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol 66: 19331938.[Crossref]
  9. Cortes S, Rolão N, Ramada J, Campino L, , 2004. PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasisusing Leishmania donovani s.l.-specific kinetoplastid primers. Trans R Soc Trop Med Hyg 98: 1217.[Crossref]
  10. Lukes J, Mauricio IL, Schonian G, Dujardin JC, Soteriadou K, Dedet JP, Kuhls K, Tintaya KW, Jirku M, Chocholova E, Haralambous C, Pratlong F, Obornik M, Horak A, Ayala FJ, Miles MA, , 2007. Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy. Proc Natl Acad Sci USA 104: 93759380.[Crossref]
  11. Schönian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, Jaffe CL, , 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47: 349358.[Crossref]
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  • Received : 06 Oct 2009
  • Accepted : 14 Jan 2010

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