1921
Volume 82, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Recently, the XE-2100 hematology analyzer was investigated in a rather small patient group; pseudoeosinophilia or abnormal white blood cell (WBC) scattergrams reported by this instrument were considered as significantly valuable diagnostic parameters in detecting vivax malaria. This study was conducted not only to assess the usefulness of pseudoeosinophilia or abnormal WBC scattergram in vivax malaria-endemic areas with large patient groups ( = 1,801) but also to investigate the correlation of parasitemia and platelet count with pseudoeosinophilia and abnormal WBC scattergrams. Of the 1,801 analyzed patients, 413 (22.9%) were found to have malaria by Wright–Giemsa stained blood smears. Overall, either pseudoeosinophilia or abnormal WBC scattergram was detected in 191 of 413 malaria patients and 4 of 1,388 patients without malaria (sensitivity = 46.2%, specificity = 99.7%). We suggest that clinical hematology laboratories using the XE-2100 analyzer should be aware of such specific parameters, even with the absence of a clinical request.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.2010.09-0560
2010-03-01
2017-09-21
Loading full text...

Full text loading...

/deliver/fulltext/14761645/82/3/412.html?itemId=/content/journals/10.4269/ajtmh.2010.09-0560&mimeType=html&fmt=ahah

References

  1. Hänscheid T, Pinto BG, Cristino JM, Grobusch MP, , 2000. Malaria diagnosis with the haematology analyser Cell-Dyn 3500: what does the instrument detect? Clin Lab Haematol 22: 259261.[Crossref]
  2. Scott CS, van Zyl D, Ho E, Meyersfeld D, Ruivo L, Mendelow BV, Coetzer TL, , 2003. Automated detection of malaria-associated intraleucocytic haemozoin by Cell-Dyn CD4000 depolarization analysis. Clin Lab Haematol 25: 7786.[Crossref]
  3. Wever PC, Henskens YM, Kager PA, Dankert J, van Gool T, , 2002. Detection of imported malaria with the Cell-Dyn 4000 hematology analyzer. J Clin Microbiol 40: 47294731.[Crossref]
  4. Hänscheid T, Melo-Cristino J, Pinto BG, , 2001. Automated detection of malaria pigment in white blood cells for the diagnosis of malaria in Portugal. Am J Trop Med Hyg 64: 290292.
  5. Hänscheid T, Pinto BG, Pereira I, Cristino JM, Valadas E, , 1999. Avoiding misdiagnosis of malaria: a novel automated method allows specific diagnosis, even in the absence of clinical suspicion. Emerg Infect Dis 5: 836838.[Crossref]
  6. Coetzer TL, Scott S, Marshall L, Liebowitz L, Wypkema E, Munster M, Tana M, Nhlangothi P, Lyons C, Mendelow BV, , 1999. Automated malaria detection by depolarization of laser light. Br J Haematol 104: 499503.[Crossref]
  7. Huh J, Jung J, Yoon H, Chung W, , 2005. Pseudoeosinophilia associated with malaria infection determined in the Sysmex XE-2100 hematology analyzer. Ann Hematol 84: 400402.[Crossref]
  8. Huh HJ, Oh GY, Huh JW, Chae SL, , 2008. Malaria detection with the Sysmex XE-2100 hematology analyzer using pseudoeosinophilia and abnormal WBC scattergram. Ann Hematol 87: 755759.[Crossref]
  9. Farcas GA, Zhong KJ, Mazzulli T, Kain KC, , 2004. Evaluation of the RealArt Malaria LC real-time PCR assay for malaria diagnosis. J Clin Microbiol 42: 636638.[Crossref]
  10. Grobusch MP, Valadas E, Hänscheid T, , 2000. Automated malaria diagnosis using pigment detection. Parasitol Today 16: 549551.[Crossref]
  11. Hänscheid T, Egan TJ, Grobusch MP, , 2007. Haemozoin: from melatonin pigment to drug target, diagnostic tool, and immune modulator. Lancet Infect Dis 7: 675685.[Crossref]
  12. Park GB, Cha YJ, , 2006. Three cases of pseudoeosinophilia associated with malaria determined in the Sysmex XE-2100 Automated Hematology Analyzer. Korean J Lab Med 26: 7780.[Crossref]
  13. Gbadegesin RA, Olumese PE, Adeyemo AA, Amodu OK, , 1998. Intraleucocytic malaria pigment and clinical severity of malaria in children. Trans R Soc Trop Med Hyg 92: 5456.[Crossref]
  14. White NJ, Tran TH, Nguyan HP, Bethell DB, Nguyen TH, Ly VC, Pham PL, Dinh XS, Phan TL, Pham TD, Day NP, , 1996. Clearance kinetics of parasites and pigment-containing leukocytes in severe malaria. Blood 88: 46944700.
  15. Kremsner PG, Mordmller BG, Metzger WG, , 1995. Malaria pigment in leucocytes. Trans R Soc Trop Med Hyg 89: 637638.[Crossref]
  16. Grobusch MP, Hanscheid T, Kramer B, Neukammer J, May J, Seybold J, Kun JF, Suttorp N, , 2003. Sensitivity of hemozoin detection by automated flow cytometry in non- and semi-immune malaria patients. Cytometry B Clin Cytom 55: 4651.[Crossref]
  17. Mendelow BV, Lyons C, Nhlangothi P, Tana M, Munster M, Wypkema E, Liebowitz L, Marshall L, Scott S, Coetzer TL, , 1999. Automated malaria detection by depolarization of laser light. Br J Haematol 104: 499503.[Crossref]
  18. Padial MM, Subirats M, Puente S, Lago M, Crespo S, Palacios G, Baquero M, , 2005. Sensitivity of laser light depolarization analysis for detection of malaria in blood samples. J Med Microbiol 54: 449452.[Crossref]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.2010.09-0560
Loading
/content/journals/10.4269/ajtmh.2010.09-0560
Loading

Data & Media loading...

  • Received : 16 Sep 2009
  • Accepted : 09 Dec 2009

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error