1921
Volume 82, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

The purpose of this study was to perform a 16S sequence-based quality control of two strain collections. 16S rRNA gene sequencing was used to verify two reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of reference collections.

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  • Received : 15 Sep 2009
  • Accepted : 26 Oct 2009
  • Published online : 05 Jan 2010

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