1921
Volume 82, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

We have developed two loop-mediated isothermal amplification (LAMP) assays for specific detection of and based on the 18S ribosomal RNA (rRNA) and the small nucleolar RNA (snoRNA) genes, respectively. The detection limit of the assays is 100 fg and 1 pg for and , respectively, with reactions conducted in 60 minutes. The two LAMP assays were used in detection of and infections in comparison with polymerase chain reaction (PCR) for DNA samples extracted from bugs collected from palm trees in Panama. Out of a total of 52 DNA samples from bugs 17 (33%) and 14 (27%) were -positive by LAMP and PCR, respectively, while, 7 (13%) and 4 (8%) were -positive by LAMP and PCR, respectively. Further evaluation of these LAMP assays is needed, especially with specimens collected from human patients as well as blood kept for transfusion purposes.

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2010-05-01
2017-09-21
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  • Received : 09 Sep 2009
  • Accepted : 08 Feb 2010

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