1921
Volume 82, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

An association between tumor necrosis factor α (TNF-α) and diarrhea was assessed in a cohort of 138 non-related Bangladeshi children who have been prospectively followed since 2001. Peripheral blood mononuclear cells (PBMCs) obtained at study entry were purified, cultured, and stimulated with soluble amebic antigen before cytokine measurement from supernatant. Higher levels of TNF-α were associated with increased risk of first ( = 0.01) and recurrent -related diarrheal episodes ( = 0.005). Children who developed diarrhea had significantly higher TNF-α protein levels than those who experienced asymptomatic infection ( value = 0.027) or no infection ( value = 0.017). Microarray studies performed using RNA isolated from acute and convalescent whole blood and colon biopsy samples revealed higher but non-significant TNF-α messenger RNA (mRNA) levels in subjects with acute diarrhea compared with convalescence. We conclude that there is an association between higher TNF-α production and diarrhea.

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  • Received : 25 Aug 2009
  • Accepted : 24 Dec 2009

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