Volume 82, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Rapid determination of glucose-6-phosphate dehydrogenase (G6PD) status is desirable when it is necessary to use a drug contraindicated in G6PD-deficient persons, such as use of primaquine for malaria prevention or treatment. The purpose of this study was to compare a new, rapid, qualitative enzyme chromatographic test for deficiency of G6PD to a standard reference method. Samples from 196 G6PD-normal persons and 50 G6PD-deficient persons were evaluated. The sensitivity of the experimental rapid test was 0.98 and the specificity was 0.98 using specimens preserved in heparin, and 0.98 and 0.97, respectively, for specimens preserved in EDTA. Positive and negative predictive values were 0.72 and 1.00, respectively, for the test for heparinized specimens and 0.65 and 1.00, respectively, for the EDTA-preserved samples. This rapid test for G6PD deficiency is a sensitive method for screening of G6PD deficiency that requires minimal training and equipment and enables rapid identification of G6PD-deficient persons.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Beutler E, , 2008. Glucose-6-phosphate dehydrogenase deficiency: a historical perspective. Blood 111: 1624.[Crossref] [Google Scholar]
  2. Beutler E, , 1994. G6PD deficiency. Blood 84: 36133636. [Google Scholar]
  3. Beutler E, Yoshida A, , 1988. Genetic variation of glucose-6-phosphate dehydrogenase: a catalog and future prospects. Medicine (Baltimore) 67: 311334.[Crossref] [Google Scholar]
  4. Cappellini MD, Fiorelli G, , 2008. Glucose-6-phosphate dehydrogenase deficiency. Lancet 371: 6474.[Crossref] [Google Scholar]
  5. Beutler E, , 1966. A series of new screening procedures for pyruvate kinase deficiency, glucose-6-phosphate dehydrogenase deficiency, and glutathione reductase deficiency. Blood 28: 553562. [Google Scholar]
  6. Beutler E, Blume KG, Kaplan C, Löhr GW, Ramot B, Valentine WN, , 1979. International Committee for Standardization in Haematology: recommended screening test for glucose-6-phosphate dehydrogenase (G-6-PD) deficiency. Br J Haematol 43: 469477.[Crossref] [Google Scholar]
  7. Mazza J, , 2001. Manual of Clinical Hematology. Philadelphia: Lippincott Williams and Wilkins, 101102. [Google Scholar]
  8. Beutler E, Mitchell M, , 1968. Special modifications of the fluorescent screening method for glucose-6-phosphate dehydrogenase deficiency. Blood 32: 816818. [Google Scholar]
  9. Tantular IS, Iwai K, Khin L, Basuki S, Horie T, Htay HH, Matsuoka H, Marwoto H, Wongsrichanalai C, Dachlan YP, Kojima S, Ishii A, Kawamoto F, , 1999. Field trials of a rapid test for G6PD deficiency in combination with a rapid diagnosis of malaria. Trop Med Int Health 4: 245250.[Crossref] [Google Scholar]
  10. Centers for Disease Control and Prevention, 2009. CDC Health Information for International Travel 2010. Atlanta: US Department of Health and Human Services, Public Health Service, 128159. [Google Scholar]
  11. Shanks GD, Oloo AJ, Aleman GM, Ohrt C, Klotz FW, Braitman D, Horton J, Brueckner E, , 2001. A new primaquine analogue, tafenoquine (WR 238605), for prophylaxis against Plasmodium falciparum malaria. Clin Infect Dis 33: 19681974.[Crossref] [Google Scholar]
  12. Uddin DE, Dickson LG, Brodine CE, , 1974. Glucose-6-phosphate dehydrogenase deficiency in military recruits. JAMA 227: 14081409.[Crossref] [Google Scholar]
  13. Chinevere TD, Murray CK, Grant E, Jr Johnson GA, Duelm F, Hospenthal DR, , 2006. Prevalence of glucose-6-phosphate dehydrogenase deficiency in U.S. Army personnel. Mil Med 171: 905907.[Crossref] [Google Scholar]
  14. Carr ME, Jr Fandre MN, Oduwa FO, , 2005. Glucose-6-phosphate dehydrogenase deficiency in two returning Operation Iraqi Freedom soldiers who developed hemolytic anemia while receiving primaquine prophylaxis for malaria. Mil Med 170: 273276.[Crossref] [Google Scholar]
  15. Mueller I, Galinski MR, Baird K, Carlton JM, Kochar DK, Alonso PL, del Portillo HA, , 2009. Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite. Lancet Infect Dis 9: 555566.[Crossref] [Google Scholar]
  16. Schwartz IK, Chin W, Newman J, Roberts JM, , 1984. Glucose-6-phosphate dehydrogenase deficiency in Southeast Asian refugees entering the United States. Am J Trop Med Hyg 33: 182184. [Google Scholar]

Data & Media loading...

  • Received : 23 Jul 2009
  • Accepted : 28 Sep 2009
  • Published online : 05 Feb 2010

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error